Interleukin 4 affects lipid metabolism and the expression of pro-inflammatory factors in mature rat adipocytes

Szczepankiewicz, Dawid; Skrzypski, Marek; Pruszyńska‐Oszmałek, Ewa; Kołodziejski, Paweł; Sassek, Maciej; Stefańska, Barbara; Nowak, Krzysztof W.; Szczepankiewicz, Aleksandra

doi:10.1016/j.imbio.2018.07.014

Cited by 18 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NKT cells in the adipose tissue play a key role in regulating TH1/TH2 response of adipose tissue, macrophage polarization, and glucose homeostasis. IL-4 mediated TH2 immune response participates in the regulation of fat by NKT cells, promoting the growth of adipose tissue and affecting lipid metabolism (Szczepankiewicz et al, 2018). In this study, LF-CQPC04 had a significant effect on these cytokines, thereby regulating lipid metabolism and promoting mice to return to normal weight.…”

Section: Discussionmentioning

confidence: 52%

Effects of Lactobacillus fermentum CQPC04 on Lipid Reduction in C57BL/6J Mice

Tan

Zhou

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Probiotics are functional foods that can effectively regulate lipid reduction and maintain body health. In this study, a strain of Lactobacillus fermentum CQPC04 (LF-CQPC04) isolated from traditional naturally fermented vegetables (Sichuan pickles) was studied, and its effects on lipid reduction in mice, as well as its mechanism of action, were observed. The results of this experiment show that LF-CQPC04 can reduce the abnormal weight gain and abnormal visceral index of mice caused by a high-fat diet. LF-CQPC04 can decrease TG (triglycerides), TC (total cholesterol), LDL-c (low-density lipoprotein cholesterol), AST (aspartate transaminase), ALT (alanine aminotransferase), and AKP (alkaline phosphatase) levels and increase HDL-c (high-density lipoprotein cholesterol) levels in the serum of high-fat mice. LF-CQPC04 can also decrease the levels of inflammatory cytokines, such as IL-6 (interleukin-6), IL-1β (interleukin-1 beta), TNF-α (tumor necrosis factor alpha), and IFN-γ (interferon gamma), and increase IL-4 and IL-10 levels in the serum of high-fat mice. The results of RT-qPCR (real-time quantitative polymerase chain reaction) and western blot experiments show that LF-CQPC04 can also down-regulate the expression of PPAR-γ (peroxisome proliferator-activated receptor gamma), C/EBP-α (CCAAT/enhances binding protein alpha) mRNA, and protein in the liver tissue of high-fat mice, while up-regulating the expression of Cu/Zn-SOD (copper/zinc superoxide dismutase), Mn-SOD (manganese superoxide dismutase), CAT (catalase), CYP7A1 (cholesterol 7 alpha hydroxylase), PPAR-α (peroxisome proliferator-activated receptor alpha), CPT1 (carnitine palmitoyl transferase 1), LPL (lipoprotein lipase), and ABCA1 (ATP-binding cassette transporter A1). Moreover, LF-CQPC04 shows stronger effects in regulating lipid reduction in mice than L-carnitine and commercial LB (Lactobacillus delbrueckii subsp. Bulgaricus) bacteria. LF-CQPC04 is beneficial for lipid reduction in animals and has good probiotic potential.

show abstract

Section: Discussionmentioning

confidence: 52%

Effects of Lactobacillus fermentum CQPC04 on Lipid Reduction in C57BL/6J Mice

Tan

Zhou

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…This is probably due to the downregulation of several critical inflammatory cytokines such as the IL-1 β and TNF- α . Previous study indicated that overproduction of inflammatory cytokines has resulted in changes in whole-body metabolism and disruption of the tissues such as the muscle and intestinal mucosa [ 46 , 47 ]. Moreover, both the IL-1 β and TNF- α can induce cell apoptosis via the intrinsic mitochondrial apoptotic pathway [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118

Lin

Xie

Chen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Purpose. β-Defensin 118 (DEFB118) is a novel host defense peptide (HDP) identified in humans. To evaluate its potentials for future utilization, the DEFB118 gene was expressed in Escherichia coli (E. coli) and the recombinant protein was fully characterized. Methods. The DEFB118 protein was obtained by heterologous expression using E. coli Rosetta (DE3). Antibacterial activity of DEFB118 was determined by using various bacterial strains. IPEC-J cells challenged by E. coli K88 were used to determine its influences on inflammatory responses. Results. The E. coli transformants yielded more than 250 μg/mL DEFB118 protein after 4 h induction by 1.0 mM IPTG. The DEFB118 was estimated by SDS-PAGE to be 30 kDa, and MALDI-TOF analysis verified that it is a human β-defensin 118. Importantly, the DEFB118 showed antimicrobial activities against both Gram-negative bacteria (E. coli K88 and E. coli DH5α) and Gram-positive bacteria (S. aureus and B. subtilis), with a minimum inhibitory concentration (MIC) of 4 μg/mL. Hemolytic assays showed that DEFB118 had no detrimental impact on cell viability. Additionally, DEFB118 was found to elevate the viability of IPEC-J2 cells upon E. coli K88 challenge. Moreover, DEFB118 significantly decreased cell apoptosis in the late apoptosis phase and downregulated the expression of inflammatory cytokines such as IL-1β and TNF-α in IPEC-J2 cell exposure to E. coli K88. Conclusions. These results suggested a novel function of the mammalian defensins, and the antibacterial and anti-inflammatory properties of DEFB118 may allow it as a potential substitute for conventionally used antibiotics or drugs.

show abstract

“…This is probably due to the down-regulation of several critical inflammatory cytokines such as the IL-1β and TNF-α. Previous study indicated overproduction of inflammatory cytokines has resulted in changes of whole-body metabolism and disruption of the tissues such as the muscle and intestinal mucosa [46,47].…”

Section: Discussionmentioning

confidence: 99%

Expression and functional characterization of a novel antimicrobial peptide: human beta-defensin118

Xie

Chen

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: β-defensin 118 (DEFB118 ) is a novel host defence peptide (HDP) identified in human. To evaluate its potentials for future utilization, the DEFB118 gene was expressed in Escherichia coli ( E. coli ) and the recombinant protein was fully characterized. Methods: The DEFB118 protein was obtained by heterologous expression using E. coli Rosetta (DE3). Antibacterical activity of DEFB118 were determined by using various bacterial strains. IPEC-J cells challenged by E. coli K88 were used to determine its influences on inflammatory responses. Results: The E. coli transformants yielded more than 250 mg/mL D EFB118 protein after 4 h induction by 1.0 mM IPTG. The DEFB118 was estimated by SDS-PAGE to be 30 kDa, and MALDI-TOF analysis verified it is a human β-defensin 118. Importantly, the DEFB118 showed antimicrobial activities against both Gram-negative bacteria ( E. coli K88 and E. coli DH5α) and Gram-positive bacteria ( S. aureus and B. subtilis ), with a minimum inhibitory concentration (MIC) of 4 μg/mL. Hemolytic assays showed that DEFB118 had no detrimental impact on cell viability. Additionally, DEFB118 was found to elevate the viability of IPEC-J2 cells upon E. coli K88 challenge. Moreover, DEFB118 significantly decreased cell apoptosis in the late apoptosis phase and down-regulated the expression of inflammatory cytokines such as the IL-1β and TNF-a in the IPEC-J2 cells exposure to E. coli K88. Conclusions: These results suggested a novel function of the mammalian defensins, and the anti-bacterial and anti-inflammatory properties of DEFB118 may allow it a potential substitute for conventionally used antibiotics or drugs.

show abstract

Interleukin 4 affects lipid metabolism and the expression of pro-inflammatory factors in mature rat adipocytes

Cited by 18 publications

References 38 publications

Effects of Lactobacillus fermentum CQPC04 on Lipid Reduction in C57BL/6J Mice

Effects of Lactobacillus fermentum CQPC04 on Lipid Reduction in C57BL/6J Mice

Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta-Defensin 118

Expression and functional characterization of a novel antimicrobial peptide: human beta-defensin118

Contact Info

Product

Resources

About