Interleukin-1 beta (IL-1 beta) modulates prostaglandin production and the natural IL-1 receptor antagonist inhibits ovulation in the optimally stimulated rat ovarian perfusion model.

Peterson, C. Matthew; Hales, Holly Ann; Hatasaka, Harry H.; Mitchell, Murray D.; Rittenhouse, Linda; Jones, Kirtly Parker

doi:10.1210/endo.133.5.7691586

Cited by 63 publications

(18 citation statements)

References 24 publications

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“…Similar results were reported by various workers in mammalian preovulatory follicles (Simon et al, 1994, Jasper et al, 1996Keck et al, 1998;Salmassi et al, 2001;Gerard et al, 2004). The expression of IL-1β, IL-6 and GM-CSF are related to several ovulation-associated events such as synthesis of plasminogen activator (PA) (Hamilton et al, 1993;Fujisaki et al, 2006;Kralisch et al, 2006), prostaglandin (Peterson et al, 1993;Neuvians et al, 2004) and nitric oxide (Yamauchi et al, 1997;Machelon and Emilie, 1997;Estevez et al, 2004) in mammals. In chicken, the PA activity in granulosa and theca cells (Jackson et al, 1993;Tischkau et al, 1996), prostaglandin F levels (Saito et al, 1987) and nitric oxide levels (Manwar et al, 2006;Sundaresan et al, 2007b) were higher in F1 follicles.…”

Section: Resultssupporting

confidence: 79%

“…Cytokines are potent autocrine regulators of granulosa or thecal cell functions (Machelon and Emilie, 1997) and are involved in the temporal and spatial remodeling of granulosa and theca cells. Further, the autocrine production of the proinflammatory cytokines was found to influence ovarian steroidogenesis (Neuvians et al, 2004;Skarzynski et al, 2005), synthesis of plasminogen activator (Hamilton et al, 1993;Fujisaki et al, 2006;Kralisch et al, 2006), synthesis of prostaglandins (Peterson et al, 1993;Neuvians et al, 2004) and synthesis of nitric oxide (Yamauchi et al, 1997;Machelon and Emilie, 1997;Estevez et al, 2004). However, the spatial expressions of cytokines in avian follicles are not studied yet.…”

Section: Introductionmentioning

confidence: 99%

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Spatial expression of chemokines and cytokines mRNA in the largest preovulatory follicle of chicken

et al. 2007

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In the present experiment, we studied the spatial expression profiles of chemokines and cytokines mRNA in the granulosa (F1Gr) and theca (F1Th) layers of the largest preovulatory follicle in chicken using semi-quantitative PCR. The mRNAs of IL-1beta, IL-6, GM-CSF, chCXCLi2, chCCLi2, chCCLi4, chCCLi7, IFN-gamma, IL-4, IL-13, IL-10 and TGF-beta2 were expressed in the granulosa (F1Gr) and theca (F1Th) layers of the largest preovulatory follicle. However, the transcripts of IL-2 were not detected in any of the samples tested. Significantly higher levels of IL-6 and GM-CSF mRNA expression were noticed in F1Gr when compared to F1Th layer. Expression of chCXCLi2, a CXC chemokine, was almost similar in F1Gr and F1Th layers. However, the expression of CCL chemokines i.e. chCCLi2, chCCLi4, chCCLi7 mRNAs were almost 2 folds higher in F1Th layer in comparison to F1Gr layer. The expression of Th2 cytokines (IL-4 and IL-13) mRNA was noticed in F1Gr and F1Th layers with higher levels in the former. Expression of IFN-gamma mRNA was noticed in F1Gr and F1Th layers. Significantly higher level of TGF-beta2 expression was observed in F1Th in comparison to F1Gr layer. It was concluded from the present study that the mRNA expression of cytokines and chemokines are differentially regulated in the granulosa and theca layers of the largest preovulatory follicle in chicken.

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Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Spatial expression of chemokines and cytokines mRNA in the largest preovulatory follicle of chicken

et al. 2007

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“…Actually, we observed that intrafollicular injection of IL-1RA did not induce the ovulation process as observed with IL-1␤. IL-1RA, rather, would delay it, as 75% of the ovulations were observed more than 55 h after treatment and no ovulation was observed before 47 h. A retarding effect of IL-1RA on the ovulation process was already suggested by the results of Peterson et al [55], who used the rat ovarian perfusion model and demonstrated that IL-1RA induced a lower ovulation rate than LH during a period of 20 h of perfusion. Moreover, it has been shown that intrafollicular injection of indomethacin, another anti-inflammatory molecule, disturbs the normal follicle development [40,56].…”

Section: Discussionmentioning

confidence: 91%

In Vivo Effect of Epidermal Growth Factor, Interleukin-1β, and Interleukin-1RA on Equine Preovulatory Follicles1

Martoriati

Duchamp

Gérard

2003

Biology of Reproduction

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Paracrine factors have significant effects during folliculogenesis. Because of various morphological features, the mare is a convenient model to study in vivo the effects of factors involved in periovulatory events. In the present work, epidermal growth factor (EGF; experiment 1, n = 49 mares) and interleukin-1beta and interleukin-1RA (IL-1beta and IL-1RA, respectively; experiment 2, n = 80 mares) were injected intrafollicularly to evaluate the influence of these factors on in vivo maturation of equine preovulatory follicles. A transvaginal ultrasound-guided injection was performed when the diameter of the dominant follicle reached 30-34 mm. In experiment 1, the four experimental groups were 1) EGF group, intrafollicular (i.f.) injection of EGF (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum; 2) control group, no injection; 3) PBS group, i.f. injection of 2 ml of PBS plus i.v. injection of physiological serum; 4) crude equine gonadotropins (CEG) group, i.f. injection of PBS plus i.v. injection of CEG (20 mg). In experiment 2, groups 3 and 4 were the same as in experiment 1, but groups 1 and 2 were changed as follows: 1) IL-1beta group, i.f. injection of IL-1beta (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum; 2) IL-1RA group, i.f. injection of IL-1RA (2 ml; 0.5 microg/ml) plus i.v. injection of physiological serum. In each experiment, cumulus-oocyte complexes from dominant/injected follicles were collected by transvaginal ultrasound-guided aspiration 38 h after intrafollicular injection. Cumulus morphology and oocyte nuclear stage were assessed. Additionally, in experiment 2, 40 mares were used to determine the time of ovulation after treatments. Our results indicate that intrafollicular injection of EGF or PBS induced lower cumulus expansion and oocyte maturation rates compared with the CEG group (P < 0.05). In experiment 2, the IL-1beta and CEG groups showed the same expansion rate, the same oocyte maturation rate, and the same ovulation distribution. On the other hand, the intrafollicular injection of IL-1RA, as PBS, did not induce follicle and cumulus-oocyte complex (COC) maturation. In conclusion, we confirmed that the technique of intrafollicular injection can be used in the mare to study the role of specific molecules. We demonstrated for the first time in mares that the injection of EGF did not influence in vivo COC maturation. In contrast, IL-1beta injection into the dominant follicle induced in vivo oocyte maturation and the ovulation process whereas IL-1RA seemed to block these mechanisms.

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“…It involved in several ovulation associated events such as synthesis of proteases, regulation nitric oxide and prostaglandin production [22]. On the other hand, previous studies has also found that hCG-induced ovulation rates were decreased in rats after treatment with IL-1 receptor antagonist (IL-1Ra) [23, 24]. Although IL-1α and IL-1β are encoded by different genes, they exert similar biological effects by binding to the same receptor (IL-1R1).…”

Section: Introductionmentioning

confidence: 99%

IL-1α Up-Regulates IL-6 Expression in Bovine Granulosa Cells via MAPKs and NF-κB Signaling Pathways

Yang¹,

Wang²,

Wang³

et al. 2017

Cell Physiol Biochem

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Background/Aims: IL-6 is one of the main cytokines in regulating ovarian follicular development and ovulation. However, the factors that regulate IL-6 expression in follicles are still unclear. The aim of this study was to elucidate the mechanisms underlying the effect of IL-1α on IL-6 expression in granulosa cells. Methods: IL-6 expression after IL-1α with/without inhibitors treatment was analyzed by RT-qPCR and ELISA. The phosphorylation of proteins induced by IL-1α was analyzed by western blot. The intracellular cAMP level was assayed by immunoassay kit. Results: IL-1α has a dose-dependent effect on IL-6 expression in granulosa cells. This promoting effect can be significantly attenuated by Erk, c-Jun, p38 and IκB proteins inhibitors, respectively. Moreover, the phosphorylation levels of Erk, c-Jun, p38 and IκBα proteins were significantly increased after IL-1α treatment. In addition, we also found that IL-1α not only reversed the cAMP attenuated IL-6 expression， but also increased IL-1α mRNA expression in granulosa cells. Conclusion: The regulation of IL-1α on IL-6 expression is mediated by activation of MAPKs and NF-κB signaling pathways. Moreover，IL-1α may regulate the ovulation-related genes expression in granulosa cells by an autocrine and/or paracrine manner.

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Interleukin-1 beta (IL-1 beta) modulates prostaglandin production and the natural IL-1 receptor antagonist inhibits ovulation in the optimally stimulated rat ovarian perfusion model.

Cited by 63 publications

References 24 publications

Spatial expression of chemokines and cytokines mRNA in the largest preovulatory follicle of chicken

Spatial expression of chemokines and cytokines mRNA in the largest preovulatory follicle of chicken

In Vivo Effect of Epidermal Growth Factor, Interleukin-1β, and Interleukin-1RA on Equine Preovulatory Follicles1

IL-1α Up-Regulates IL-6 Expression in Bovine Granulosa Cells via MAPKs and NF-κB Signaling Pathways

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