Interfacial Compression-Dependent Merging of Two Miscible Microdroplets in an Asymmetric Cross-Junction for In Situ Microgel Formation

Jang, Yeongseok; Cha, Chaenyung; Jung, Ju Yeon; Oh, Jonghyun

doi:10.1007/s13233-019-7013-8

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…To evaluate the viability of encapsulated cells, the cells were fluorescently labeled with calcein-AM and ethidium homodimer-1 to distinguish live (green) and red (dead) cells, respectively (LIVE/DEAD Cell Viability/Cytotoxicity Kit, Thermo Fisher) [ 44 ]. The cells were visualized under a fluorescence microscope (XDS-3FL, Optika).…”

Section: Methodsmentioning

confidence: 99%

Multiscale Control of Nanofiber-Composite Hydrogel for Complex 3D Cell Culture by Extracellular Matrix Composition and Nanofiber Alignment

Choi,

Yun,

Song

et al. 2024

Biomater Res

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In order to manipulate the complex behavior of cells in a 3-dimensional (3D) environment, it is important to provide the microenvironment that can accurately portray the complexity of highly anisotropic tissue structures. However, it is technically challenging to generate a complex microenvironment using conventional biomaterials that are mostly isotropic with limited bioactivity. In this study, the gelatin-hyaluronic acid hydrogel incorporated with aqueous-dispersible, short nanofibers capable of in situ alignment is developed to emulate the native heterogeneous extracellular matrix consisting of fibrous and non-fibrous components. The gelatin nanofibers containing magnetic nanoparticles, which could be aligned by external magnetic field, are dispersed and embedded in gelatin-hyaluronic acid hydrogel encapsulated with dermal fibroblasts. The aligned nanofibers via magnetic field could be safely integrated into the hydrogel, and the process could be repeated to generate larger 3D hydrogels with variable nanofiber alignments. The aligned nanofibers in the hydrogel can more effectively guide the anisotropic morphology (e.g., elongation) of dermal fibroblasts than random nanofibers, whereas myofibroblastic differentiation is more prominent in random nanofibers. At a given nanofiber configuration, the hydrogel composition having intermediate hyaluronic acid content induces myofibroblastic differentiation. These results indicate that modulating the degree of nanofiber alignment and the hyaluronic acid content of the hydrogel are crucial factors that critically influence the fibroblast phenotypes. The nanofiber-composite hydrogel capable of directional nanofiber alignment and tunable material composition can effectively induce a wide array of phenotypic plasticity in 3D cell culture.

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Section: Methodsmentioning

confidence: 99%

Multiscale Control of Nanofiber-Composite Hydrogel for Complex 3D Cell Culture by Extracellular Matrix Composition and Nanofiber Alignment

Choi,

Yun,

Song

et al. 2024

Biomater Res

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“…2. A wider segment is also added just after the second dispersed phase channel to facilitate droplet merging [42][43][44].…”

Section: Designmentioning

confidence: 99%

Biocompatible Janus Microparticle Synthesis in a Microfluidic Device

Saqib,

Tufan,

Orsel

et al. 2024

Preprint

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Janus particles are popular in recent years due to their anisotropic physical and chemical properties. Even though there are several established synthesis methods for Janus particles, microfluidics-based methods are convenient and reliable due to low reagent consumption, monodispersity of the resultant particles and efficient control over reaction conditions. In this work a simple droplet-based microfluidic technique is utilized to synthesize magnetically anisotropic TiO2-Fe2O3 Janus microparticles. Two droplets containing reagents for Janus particle were merged by using an asymmetric device such that the resulting droplet contained the constituents within its two hemispheres distinct from each other. The synthesized Janus particles were observed under the optical microscope and the scanning electron microscope. Moreover, a detailed in vitro characterization of these particles was completed, and it was shown that these particles have a potential use for biomedical applications.

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“…Jang et al [52] proposed a new in situ crosslinking methodology for the fabrication of microgels by merging two droplets of different viscosities in an asymmetric cross-junction microfluidic device. Thus, oxidized dextran (ODX) and N-carboxymethyl chitosan (N-CEC) were mixed to undergo an in situ crosslinking via a Schiff base reaction, resulting in microgel formation.…”

Section: Chitosanmentioning

confidence: 99%

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

2021

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Natural polymers have been widely used for biomedical applications in recent decades. They offer the advantages of resembling the extracellular matrix of native tissues and retaining biochemical cues and properties necessary to enhance their biocompatibility, so they usually improve the cellular attachment and behavior and avoid immunological reactions. Moreover, they offer a rapid degradability through natural enzymatic or chemical processes. However, natural polymers present poor mechanical strength, which frequently makes the manipulation processes difficult. Recent advances in biofabrication, 3D printing, microfluidics, and cell-electrospinning allow the manufacturing of complex natural polymer matrixes with biophysical and structural properties similar to those of the extracellular matrix. In addition, these techniques offer the possibility of incorporating different cell lines into the fabrication process, a revolutionary strategy broadly explored in recent years to produce cell-laden scaffolds that can better mimic the properties of functional tissues. In this review, the use of 3D printing, microfluidics, and electrospinning approaches has been extensively investigated for the biofabrication of naturally derived polymer scaffolds with encapsulated cells intended for biomedical applications (e.g., cell therapies, bone and dental grafts, cardiovascular or musculoskeletal tissue regeneration, and wound healing).

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Interfacial Compression-Dependent Merging of Two Miscible Microdroplets in an Asymmetric Cross-Junction for In Situ Microgel Formation

Cited by 10 publications

References 42 publications

Multiscale Control of Nanofiber-Composite Hydrogel for Complex 3D Cell Culture by Extracellular Matrix Composition and Nanofiber Alignment

Multiscale Control of Nanofiber-Composite Hydrogel for Complex 3D Cell Culture by Extracellular Matrix Composition and Nanofiber Alignment

Biocompatible Janus Microparticle Synthesis in a Microfluidic Device

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

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