2010
DOI: 10.1126/science.1192588
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Abstract: In bacteria, the rate of cell proliferation and the level of gene expression are intimately intertwined. Elucidating these relations is important both for understanding the physiological functions of endogenous genetic circuits and for designing robust synthetic systems. We describe a phenomenological study that reveals intrinsic constraints governing the allocation of resources toward protein synthesis and other aspects of cell growth. A theory incorporating these constraints can accurately predict how cell p… Show more

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Cited by 1,203 publications
(2,163 citation statements)
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References 24 publications
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“…If one of these resources is limiting, then a cell that invests more resources in one metabolic process must invest fewer resources in other processes. Recent studies support the existence of such competitive resource conflicts (Dekel and Alon, 2005;Scott et al, 2010), but the specific intracellular resources that were under competition were not identified. Below we discuss two that might be of relevance.…”
Section: Biochemical Conflicts That Could Promote Metabolic Specializmentioning
confidence: 99%
“…If one of these resources is limiting, then a cell that invests more resources in one metabolic process must invest fewer resources in other processes. Recent studies support the existence of such competitive resource conflicts (Dekel and Alon, 2005;Scott et al, 2010), but the specific intracellular resources that were under competition were not identified. Below we discuss two that might be of relevance.…”
Section: Biochemical Conflicts That Could Promote Metabolic Specializmentioning
confidence: 99%
“…Indeed, for complex network systems (such as a leaf), such observations may provide insights that are not afforded by simple mutation of an endogenous system in which internal feedback loops within the network may act to dampen any response. In a manner analogous to computational approaches, in which parameter space can be explored to identify factors that robustly influence a particular feature of a system, by artificially manipulating growth it is possible to characterize the spatial and temporal limits within which this parameter acts to influence the system (Scott et al, 2010). Having characterized these limits, it may be possible to 'reverse engineer' the system to discover the endogenous pathways that regulate that parameter.…”
Section: Approaches To Manipulating and Understanding Leaf Formmentioning
confidence: 99%
“…Much of our understanding of bacterial and yeast life cycles stems from monitoring their proliferation in time and the most routine way of doing so is using optical density (OD) measurements. The applications of such measurements range from routine checks during different cloning techniques 1 ; through studying cellular physiology and metabolism 2,3 ; to determining the growth rate for antibiotic dosage 4,5 ; and monitoring of biomass accumulation during bio-industrial fermentation 6 . Here we introduce a set of calibration techniques that take into account the relevant parameters affecting OD measurements, including at high culture densities, in a range of conditions commonly used by researchers.…”
mentioning
confidence: 99%