Interchloroplast communications in Chara are suppressed under the alkaline bands and are relieved after the plasma membrane excitation

Булычев, А. А.; Krupenina, Natalia A.

doi:10.1016/j.bioelechem.2019.05.006

Cited by 9 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the current knowledge on photoinduced ion transport across the chloroplast envelope membranes in vivo is insufficient to include ion fluxes into the scheme of cyclosis‐mediated regulation of photosynthesis. Nevertheless, available evidence suggests that the transmission of the regulatory signal is sensitive to cytoplasmic pH whose distribution is not uniform in cell regions underlying the alkaline and acidic zones of the extracellular pH pattern (Bulychev & Rybina, 2018; Bulychev & Krupenina, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies with Chara revealed very slow stages of F′ fluorescence induction under the exposure of internodal cells to dim light (approximately 10 μmol m −2 s −1 ) (Bulychev & Komarova, 2015; Bulychev & Krupenina, 2019). The appearance of this S‐M‐T fluorescence transient correlated with the development of spatial connectivity, as observed from longitudinal propagation of the F′ fluorescence wave.…”

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Effects of chloroplast–cytoplasm exchange and lateral mass transfer on slow induction of chlorophyll fluorescence in Characeae

Булычев

Cherkashin

Shapiguzov

et al. 2021

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

Rapid cytoplasmic streaming in characean algae mediates communications between remote cell regions exposed to uneven irradiance. The metabolites exported from brightly illuminated chloroplasts spread along the internode with the liquid flow and cause transient changes in chlorophyll fluorescence at cell areas that are exposed to dim light or placed shortly in darkness. The largest distance to which the photometabolites can be transported has not yet been determined. Neither is it known if lateral transport has an influence on the induction of chlorophyll fluorescence. In this study, the relations between spatial connectivity of anchored chloroplasts in characean internodes and fluorescence induction curves were examined. Connectivity between remote cell parts was pronounced upon illumination of a cell spot at a distance up to 10 mm from the area of fluorescence measurement, provided the spot was located upstream in the cytoplasmic flow. Spatial interactions between distant cell sites were also manifested in strikingly different slow stages of fluorescence induction caused by narrow‐ and wide‐field illumination. Cytochalasin D, cooling of bath solution, and inactivation of light‐dependent envelope transporters were used to disturb cyclosis‐mediated spatial interactions. Although fluorescence induction curves induced by narrow‐ and wide‐field illumination differed greatly under control conditions, they became similar after the inhibition of cyclosis with cytochalasin D. The results indicate that cytoplasmic streaming not only drives the lateral translocation of photometabolites but also promotes the export of reducing power from illuminated chloroplasts due to flushing the chloroplast surface and keeping sharp concentration gradients.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%