Intercellular Waves of Communication

Sanderson, MJ

doi:10.1152/physiologyonline.1996.11.6.262

Cited by 45 publications

(42 citation statements)

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“…Calcium oscillations may represent a general mechanism by which cells communicate via inositol 1,4,5-triphosphate through gap junctions [10]. In the present study, the ATP-induced [Ca2+]i increase in calcium oscillations was synchronized among adjacent cells in the wing cell layer.…”

Section: Discussionsupporting

confidence: 52%

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Application of Real-Time Confocal Microscopy to Observations of ATP-Induced Ca2+-Oscillatory Fluctuations in Intact Corneal Epithlial Cells.

Kimura

Yamashita

Nishimura

et al. 1999

Acta Histochem. Cytochem

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Section: Discussionsupporting

confidence: 52%

“…Spikes in the wing cells did not affect the [Ca2+]i dynamics of the superficial cells. These findings indicate that communication was progressively restricted by a repetitive increase in [Ca2+]; [10], and that the propagation of the [Ca2+]i increase was not an infinite phenomenon.…”

Section: Resultsmentioning

confidence: 69%

Application of Real-Time Confocal Microscopy to Observations of ATP-Induced Ca2+-Oscillatory Fluctuations in Intact Corneal Epithlial Cells.

Kimura

Yamashita

Nishimura

et al. 1999

Acta Histochem. Cytochem

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“…Such signaling is now well established to occur by two distinct pathways -gap junctional and paracrine -and both may operate in a given situation (Boitano et al, 1992;Sanderson, 1996;Giaume and Venance, 1998;Homolya et al, 2000;Braet et al, 2001;Jorgensen et al, 2002;Schuster et al, 2003;Bennett et al, 2006;Henriksen et al, 2006;Scemes and Giaume, 2006). The former involves intercellular diffusion through gap junction channels of a molecular signal, usually considered to be IP3 and/or Ca.…”

Section: Junctional Channelsmentioning

confidence: 99%

Connexin channel permeability to cytoplasmic molecules

Harris¹

2007

Progress in Biophysics and Molecular Biology

411

346

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Connexin channels are known to be permeable to a variety of cytoplasmic molecules. The first observation of second messenger junctional permeability, made ∼30 years ago, sparked broad interest in gap junction channels as mediators of intercellular molecular signaling. Since then, much has been learned about the diversity of connexin channels with regard to isoform diversity, tissue and developmental distribution, modes of channel regulation, assembly and expression, biochemical modification and permeability, all of which appear to be dynamically regulated. This information has expanded the potential roles of connexin channels in development, physiology and disease, and made their elucidation much more complex -30 years ago such an orchestra of junctional dynamics was unanticipated. Only recently, however, have investigators been able to directly address, in this more complex framework, the key issue: What specific biological molecules, second messengers and others, are able to permeate the various types of connexin channels, and how well? An important related issue, given the ever-growing list of connexin-related pathologies, is how these permeabilities are altered by disease-causing connexin mutations. Together, many studies show that a variety of cytoplasmic molecules can permeate the different types of connexin channels. A few studies reveal differences in permeation by different molecules through a particular type of connexin channel, and differences in permeation by a particular molecule through different types of connexin channels. This article describes and evaluates the various methods used to obtain these data, presents an annotated compilation of the results, and discusses the findings in the context of what can be inferred about mechanism of selectivity and potential relevance to signaling. The data strongly suggest that highly specific interactions take place between connexin pores and specific biological molecular permeants, and that those interactions determine which cytoplasmic molecules can permeate and how well. At this time, the nature of those interactions is unclear. One hopes that with more detailed permeability and structural information, the specific molecular mechanisms of the selectivity can be elucidated.

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“…Numerous studies have documented that perturbation of gap junctional communication in tissues known to be sites of CF manifestations is associated with the dysfunction of epithelial cells. For example, blockade of gap junctional communication alters the synchronized ciliary beating of tracheal cells and enhances the basal release of digestive enzymes by pancreatic acinar cells (23,48). The CFPAC-1/PLJ-CFTR lines are known to have retained typical features of the native cells from which they derived (24,25).…”

Section: Figurementioning

confidence: 99%

“…In keeping with this view, several studies have shown that agents that activate or inhibit CFTR activity in various epithelial cells also alter their capability for intercellular communication (17)(18)(19). Conversely, disruption of gap junctional communication has been associated with altered functions in tissues in which manifestations of CF occur (20)(21)(22)(23). However, whether epithelial cells devoid of functional CFTR exhibit abnormal intercellular communication is not known.…”

Section: Introductionmentioning

confidence: 99%

Defective regulation of gap junctional coupling in cystic fibrosis pancreatic duct cells

Chanson

Scerri²,

Suter³

1999

J. Clin. Invest.

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The cystic fibrosis (CF) gene encodes a cAMP-gated Cl -channel (cystic fibrosis transmembrane conductance regulator [CFTR]) that mediates fluid transport across the luminal surfaces of a variety of epithelial cells. We have previously shown that gap junctional communication and Cl -secretion were concurrently regulated by cAMP in cells expressing CFTR. To determine whether intercellular communication and CFTR-dependent secretion are related, we have compared gap junctional coupling in a human pancreatic cell line harboring the ∆F508 mutation in CFTR and in the same cell line in which the defect was corrected by transfection with wild-type CFTR. Both cell lines expressed connexin45 (Cx45), as evidenced by RT-PCR, immunocytochemistry, and dual patch-clamp recording. Exposure to agents that elevate intracellular cAMP or specifically activate protein kinase A evoked Cl -currents and markedly increased junctional conductance of CFTR-expressing pairs, but not in the parental cells. The latter effect, which was caused by an increase in single-channel activity but not in unitary conductance of Cx45 channels, was not prevented by exposing CFTR-expressing cells to a Cl -channel blocker. We conclude that expression of functional CFTR restored the cAMP-dependent regulation of junctional conductance in CF cells. Direct intercellular communication coordinates multicellular activity in tissues that are major targets of CF manifestations. Consequently, defective regulation of gap junction channels may contribute to the altered functions of tissues affected in CF.

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Intercellular Waves of Communication

Abstract: Intercellular Ca2+ waves may be propagated through multicellular systems by the diffusion, through gap junctions, of the second messenger inositol 1, 4, 5-trisphosphate (IP3). This intercellular IP3 signaling provides a mechanism by which cooperative cell activity may be coordinated.

Cited by 45 publications

References 0 publications

Application of Real-Time Confocal Microscopy to Observations of ATP-Induced Ca2+-Oscillatory Fluctuations in Intact Corneal Epithlial Cells.

Application of Real-Time Confocal Microscopy to Observations of ATP-Induced Ca2+-Oscillatory Fluctuations in Intact Corneal Epithlial Cells.

Connexin channel permeability to cytoplasmic molecules

Defective regulation of gap junctional coupling in cystic fibrosis pancreatic duct cells

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