Interactive Effect of Elevated CO2 and Moisture Stress on Anatomical Configuration in  Brassica Species

Das, Ranjan

doi:10.9734/ijecc/2021/v11i230370

Cited by 2 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is well documented that drought stress resulted in enhanced level of SOD (Abedi and Pakniyat, 2010) and POD in plant species like wheat (Csiszar et al 2005), brassica (Das and Uprety, 2006) and poplar (Xiao et al 2008). The current data also indicated the higher levels of POD and SOD in sunflower after drought stress which is in agreement with the reported results.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics and Expression Analysis of KCS Genes Under Drought Stress in Sunflower (Helianthus Annuus L.)

2022

THE JAPS

View full text Add to dashboard Cite

Water stress is considered as the main environmental factor which badly affects growth of sunflower plant. It is a water sensitive plant whose yield is greatly affected by drought stress. Several studies have been done to understand the mechanism of drought stress tolerance in plants, and exploring the role of stress tolerant genes is one of them. KCS genes, responsible for wax biosynthesis, have been reported to be involved in drought stress tolerance mechanism. In this study, expression profiling of KCS genes was done to understand their role in stress mechanism. Comparative genomics studies of KCS genes were carried out in sunflower and Arabidopsis by constructing phylogenetic tree. They were divided into six clades, however they were present in the same clade showing similarities between Arabidopsis and sunflower. It was further confirmed by Synteny analysis and concluded that KCS genes in both species share the same evolutionary origin. Further, they were amplified in sunflower by using gene specific primers. Five genes, i.e. KCS2, KCS4, KCS5, KCS10 and KCS18 were successfully amplified in sunflower variety FH-593. Then, sunflower plants were subjected to drought stress and expression profiling of amplified KCS genes was carried out by Real Time PCR. All the five genes were up-regulated under drought showing their role in stress conditions; however, the expression level of each gene was varied. Maximum relative expression was found for KCS4 gene in T1, i.e. 19 fold as compared to control. The expression of other genes was found in decreasing order as indicated KCS4>KCS2>KCS5>KCS10>KCS18 (relative expression: 19, 12, 5, 4 and 3 respectively). Biochemical analyses were also performed and significant variation was found among treatments and control. Total chlorophyll contents were decreased under drought stress while antioxidants like catalase, peroxidase, superoxide dismutase and proline were increased. Our results showed the role of KCS genes in drought stress which is first ever report in sunflower. This study concluded that KCS genes have role in drought stress tolerance and their expression is significantly up-regulated under stress conditions. The information presented here may help to further characterize KCS genes and their subsequent use to engineer drought stress in crop plants.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative Genomics and Expression Analysis of KCS Genes Under Drought Stress in Sunflower (Helianthus Annuus L.)

2022

THE JAPS

View full text Add to dashboard Cite

show abstract

“…The approach of Das and Uprety [ 47 ] was used to calculate the membrane stability index. Small, round discs of freshly cut leaves were created.…”

Section: Methodsmentioning

confidence: 99%

Nitric Oxide and Proline Modulate Redox Homeostasis and Photosynthetic Metabolism in Wheat Plants under High Temperature Stress Acclimation

Sehar

Mir

Khan

et al. 2023

Plants

View full text Add to dashboard Cite

The effects of exogenously-sourced NO (nitric oxide, as 100 µM SNP) and proline (50 mM) in the protection of the photosynthetic performance of wheat (Triticum aestivum L.) plants against heat stress were investigated. The study focused on the mechanisms of proline accumulation, activity, gene expression of antioxidant enzymes, and NO generation. Plants were exposed to a temperature of 40 °C for 6 h per day over 15 days, then allowed to recover at 28 °C. Heat-stressed plants showed increased oxidative stress, with higher levels of H2O2 and TBARS (thiobarbituric acid reactive substances) and increased proline accumulation, ACS activity, ethylene evolution, and NO generation, which in turn leads to increased accumulation of antioxidant enzymes and reduced photosynthetic attributes. In the tested wheat cultivar, the exogenous application of SNP and proline under heat stress improved the photosynthesis and reduced oxidative stress by enhancing the enzymatic antioxidant defense system. Potentially, the promoter AOX (alternative oxidase) played a role in maintaining redox homeostasis by lowering H2O2 and TBARS levels. The genes for GR antioxidant and photosystem II core protein encoding psbA and psbB were highly up-regulated in nitric oxide and proline treated heat-stressed plants, indicating that ethylene positively impacted photosynthesis under high temperature stress. Moreover, nitric oxide supplementation under high temperature stress optimized ethylene levels to regulate the assimilation and metabolism of proline and the antioxidant system, lowering the adverse effects. The study showed that nitric oxide and proline increased high temperature stress tolerance in wheat by increasing the osmolytes accumulation and the antioxidant system, resulting in enhanced photosynthesis.

show abstract

Interactive Effect of Elevated CO2 and Moisture Stress on Anatomical Configuration in Brassica Species

Cited by 2 publications

References 16 publications

Comparative Genomics and Expression Analysis of KCS Genes Under Drought Stress in Sunflower (Helianthus Annuus L.)

Comparative Genomics and Expression Analysis of KCS Genes Under Drought Stress in Sunflower (Helianthus Annuus L.)

Nitric Oxide and Proline Modulate Redox Homeostasis and Photosynthetic Metabolism in Wheat Plants under High Temperature Stress Acclimation

Contact Info

Product

Resources

About