Interactions of Two Ethidium Derivatives with Calf Thymus DNA and Serum Albumins Using Fluorescence Quenching Method

Akbay, N.; Seferoglu, Z.; Gökoğlu, Elmas

doi:10.2174/1573411011006040334

Cited by 2 publications

(2 citation statements)

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“…17,20,22 As reported by our group and other research groups, most of the quenching is in good accordance with this theory and fits the results in the distinguishing experiments. 4,5,[23][24][25] However, as some exceptions appeared when studying the interactions between some novel acylhydrazone derivatives and serum albumin in our group, 10 and in other reports, 46,47 a doubt has been raised. Can this theory cover all the quenching behaviors of drugs towards protein, or in another words, do all the quenching behaviors exactly fit the principle described in this theory?…”

Section: Introductionmentioning

confidence: 64%

The adsorption of an anticancer hydrazone by protein: an unusual static quenching mechanism

Tian

Jiang

et al. 2012

RSC Adv.

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Section: Introductionmentioning

confidence: 64%

The adsorption of an anticancer hydrazone by protein: an unusual static quenching mechanism

Tian

Jiang

et al. 2012

RSC Adv.

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“…Some techniques are commonly used to detect the interaction between DNA and small molecules, including fluorescence spectroscopy, 14 UV spectrophotometry, 15 circular dichroism (CD), 16 Fourier transform infrared (FT-IR) spectroscopy, 17 nuclear magnetic resonance (NMR), 18 and electrospray ionization mass spectrometry (EI-MS). 19 Among these techniques, UVÀvis absorption and fluorescence spectroscopy are well-known and employed as simple but sensitive methods.…”

Section: ' Introductionmentioning

confidence: 99%

Molecular Spectroscopic Studies of Farrerol Interaction with Calf Thymus DNA

Zhang

Wang

et al. 2011

J. Agric. Food Chem.

168

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The interaction between farrerol and calf thymus DNA in a pH 7.4 Tris-HCl buffer was investigated with the use of neutral red (NR) dye as a spectral probe by UV-vis absorption, fluorescence, and circular dichroism (CD) spectroscopy, as well as viscosity measurements and DNA melting techniques. It was found that farrerol molecules could intercalate into the base pairs of DNA as evidenced by decreases in iodide quenching effect and single-stranded DNA (ssDNA) quenching effect, induced CD spectral changes, and significant increases in relative viscosity and denaturation temperature of DNA. Furthermore, the spectral data matrix of the competitive reaction between farrerol and NR with DNA was resolved with an alternative least-squares (ALS) algorithm, and the concentration profiles in the reaction and the corresponding pure spectra for three species (farrerol, NR, and DNA-NR complex) were obtained. This ALS analysis demonstrated the intercalation of farrerol to the DNA by substituting for NR in the DNA-NR complex. Moreover, the thermodynamic parameters enthalpy change (ΔH°) and entropy change (ΔS°) were calculated to be -16.49 ± 0.51 kJ mol(-1) and 32.47 ± 1.02 J mol(-1) K(-1) via the van't Hoff equation, which suggested that the binding of farrerol to DNA was driven mainly by hydrophobic interactions and hydrogen bonds.

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Interactions of Two Ethidium Derivatives with Calf Thymus DNA and Serum Albumins Using Fluorescence Quenching Method

Cited by 2 publications

References 0 publications

The adsorption of an anticancer hydrazone by protein: an unusual static quenching mechanism

The adsorption of an anticancer hydrazone by protein: an unusual static quenching mechanism

Molecular Spectroscopic Studies of Farrerol Interaction with Calf Thymus DNA

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