volume 66, issue 9, P5485-5491 1992
DOI: 10.1128/jvi.66.9.5485-5491.1992
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M Yoakim, W Hou, Y Liu, C L Carpenter, R Kapeller, B S Schaffhausen

Abstract: The binding of phosphatidylinositol-3-kinase to the polyomavirus middle T antigen is facilitated by tyrosine phosphorylation of middle T on residue 315. The pp85 subunit of phosphatidylinositol-3-kinase contains two SH2 domains, one in the middle of the molecule and one at the C terminus. When assayed by blotting with phosphorylated middle T, the more N-terminal SH2 domain is responsible for binding to middle T. When assayed in solution with glutathione S transferase fusions, both SH2s are capable of binding p…

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