Interactions of Paracoccidioides brasiliensis with host cells: recent advances

Mendes-Giannini, María José Soares; Silva, Juliana Leal Monteiro da; Silva, Julhiany de Fátima da; Donofrio, Fabiana Cristina; Miranda, Elaine Toscano; Andreotti, Patrícia Ferrari; Soares, Christiane Pienna

doi:10.1007/s11046-007-9074-z

Cited by 57 publications

(47 citation statements)

References 78 publications

(105 reference statements)

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“…brasiliensis to adhere to and invade epithelial cells (Mendes-Giannini et al, 2008), and the adhesion phenomenon is variable depending on the isolate (Andreotti et al, 2005;Hanna et al, 2000). Adhesion of P. brasiliensis to host tissues is a crucial step in the establishment of PCM.…”

Section: R Peres Da Silva and Othersmentioning

confidence: 99%

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Silva

Matsumoto

Braz

et al. 2011

Journal of Medical Microbiology

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Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, one of the most important systemic fungal diseases in Latin America. This initiates in lung tissue and can subsequently disseminate to other tissues. Clinical manifestations range from localized forms to disseminated disease that can progress to lethality, probably depending on the relationships among the virulence of the fungus, the immune response and the ability to interact with the surface structures and invade epithelial cells and mononuclear cells of the host. It is generally regarded as a multifocal disease, with oral lesions as the prominent feature. The aim of this study was to evaluate P. brasiliensis yeast infection in normal oral keratinocytes (NOKs). The differential expression of mRNAs and proteins was also determined when the fungus was placed in contact with the cell in order to characterize differentially expressed genes and proteins during P. brasiliensis infection. After contact with NOKs, the fungus appeared to induce alterations in the cells, which showed cellular extensions and cavitations, probably resulting from changes in the actin cytoskeleton seen at 5 and 8 h after infection. Levels of protein expression were higher after reisolation of the fungus from infected NOK culture compared with culture of the fungus in medium. The analysis identified transcripts related to 19 proteins involved in different biological processes. Transcripts were found with multiple functions including induction of cytokines, protein metabolism, alternative carbon metabolism, zinc transport and the stress response during contact with NOKs. The proteins found suggested that the yeast was in a stress situation, as indicated by the presence of RDS1. Nevertheless, the yeast seemed to be proliferating and metabolically active, as shown by the presence of a proteasome, short-chain acetylator, glucosamine-6-phosphate isomerase and ADP/ATP carrier transcripts. Additionally, metabolic pathways may have been activated in order to eliminate toxic substances from the cell as a zinc transporter was detected, which is a potential target for the development of future drugs. INTRODUCTIONParacoccidioides brasiliensis is a thermodimorphic pathogenic fungus, the aetiological agent of paracoccidioidomycosis (PCM), an endemic disease geographically limited to Latin America and one of the most prevalent human deep systemic mycoses found in Brazil, Colombia and Venezuela (San-Blas et al., 2002). The transition from mycelium at 25 u C to yeast at 37 u C is essential for P. brasiliensis to establish the disease (Franco et al., 1997). P. brasiliensis may be inhaled and, once in the lungs, the fungus initiates a process of morphological transition into the pathogenic yeast form (San-Blas et al., 2002).PCM has a multiplicity of clinical presentations, from cutaneous to systemic forms, and can attack various tissues, especially the lungs (Benard & Franco, 2007). Oral mucosal lesions are commonly multiple, with a granular, mulberry-like surface and microulcerations, and ...

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Section: R Peres Da Silva and Othersmentioning

confidence: 99%

Differential gene expression analysis of Paracoccidioides brasiliensis during keratinocyte infection

Silva

Matsumoto

Braz

et al. 2011

Journal of Medical Microbiology

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“…This invasion was associated with severe ATI as a primary event that was characterized by a decrease in thymus weight, an increase in number of histiocytes, limited distinction of the cortico-medullary junction along with degenerative changes in the thymic cortex . Mechanistically, ATI during experimental paracoccidioidomycosis was caused by apoptosis, as indicated by a significant increase in the apoptotic index as well as typical cellular changes that were triggered by autophagic programmed cell death (Mendes-Giannini et al 2008;Souto et al 2003). Gayathri et al (2011) investigated the effect of an extract of a fern known as Selaginella involvens that was shown to have thymus growth-stimulatory properties and found that this substance protected cortisone-treated immuno-compromised mice from an infection with Aspergillus fumigatus.…”

Section: Fungal Infections and Atimentioning

confidence: 99%

“…However, ATI in experimental animal models has been reported to occur not only during gestational periods (Kunzmann et al 2010;between ATI in adult experimental subjects and ageassociated thymic atrophy during the mid-phase of life in human beings is that the former occurs due to an increase in the death of thymocytes and has the ability to recover after the removal of the insult (Duan et al 2015;Lee et al 2011;Park et al 2007), whereas the latter is characterized by the substitution of lymphoid tissue with fat (adipose tissue) in a progressive time-dependent manner (Bodey et al 1997;Henry and Anderson 1987;Quaglino et al 2014). ATI is a common and well-known feature in various fungal Mendes-Giannini et al 2008;Souto et al 2003), viral (Duan et al 2015;Falkenberg et al 2014;Gao et al 2015) and bacterial infectious diseases (LeyvaRangel et al 2015;Ross et al 2012;Savino 2006). ATI can also be caused by immune suppression (Billard et al 2011;Zhou et al 2016), hunger (Gavia-García et al 2015;Savino et al 2007), chemotherapy, radiography (DeBo et al 2015;Lynch et al 2009), pregnancy (Ekin et al 2016;Jacques et al 2014Jacques et al , 2015, transplant rejection (Gracia-Ahufinger et al 2015;Krenzien et al 2015) and other severe disease conditions (Wang et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Acute Thymic Involution and Mechanisms for Recovery

Ansari

Liu

2017

Arch. Immunol. Ther. Exp.

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“…The usefulness of murine models to discern important innate immune mechanisms against P. brasiliensis infections is summarized by Calich et al [8] in order to explain how most of the exposed individuals escape from overt disease. Mendes-Giannini et al [9] provide an exquisite description of P. brasiliensis-epithelial cell interactions to underscore the important virulence factors of the fungus. A wealth of data emerging from DNA technology is summarized by Silva et al [10] with focus on genes putatively related to P. brasiliensis-host adaptations.…”

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confidence: 99%