Interactions of oxidized multiwalled carbon nanotube with cadmium on zebrafish cell line: The influence of two co-exposure protocols on in vitro toxicity tests

Morozesk, Mariana; Franqui, Lidiane S.; Mansano, Adrislaine S.; Martinez, Diego Stéfani T.

doi:10.1016/j.aquatox.2018.05.002

Cited by 34 publications

(13 citation statements)

References 70 publications

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“…While this eliminates many potential variables, factors that could attenuate or exacerbate any direct mitogenomic effects from the MWCNTs in an intact organism are beyond the scope of this study. These factors include deposition 61 of material in the lungs, other cells/tissues 62 (e.g., resident macrophages), inflammatory responses 63 to the nanomaterials, and co-exposures with other pollutants/chemicals/pathogens 64 . Another inherent limitation is the use of a submerged epithelial culture model instead of an (arguably more physiologically-relevant 65 ) air-liquid interface model.…”

Section: Discussionmentioning

confidence: 99%

Multi-walled carbon nanotubes upregulate mitochondrial gene expression and trigger mitochondrial dysfunction in primary human bronchial epithelial cells

et al. 2019

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Nanomaterials are a relatively new class of materials that acquire novel properties based on their reduced size. While these materials have widespread use in consumer products and industrial applications, the potential health risks associated with exposure to them remain to be fully characterized. Carbon nanotubes are among the most widely used nanomaterials and have high potential for human exposure by inhalation. These nanomaterials are known to penetrate the cell membrane and interact with intracellular molecules, resulting in a multitude of documented effects, including oxidative stress, genotoxicity, impaired metabolism, and apoptosis. While the capacity for carbon nanotubes to damage nuclear DNA has been established, the effect of exposure on mitochondrial DNA (mtDNA) is relatively unexplored. In this study, we investigated the potential of multi-walled carbon nanotubes (MWCNTs) to impair mitochondrial gene expression and function in human bronchial epithelial cells (BECs). Primary BECs were exposed to subcytotoxic doses (up to 3 μg/ml) of MWCNTs for 5 days and assessed for changes in expression of all mitochondrial protein-coding genes, heteroplasmies, and insertion/deletion mutations (indels). Exposed cells were also measured for cytotoxicity, metabolic function, mitochondrial abundance, and mitophagy. We found that MWCNTs upregulated mitochondrial gene expression, while significantly decreasing oxygen consumption rate and mitochondrial abundance. Confocal microscopy revealed induction of mitophagy by 2 hours of exposure. Mitochondrial DNA heteroplasmy and insertion/deletion mutations were not significantly affected by any treatment. We conclude that carbon nanotubes cause mitochondrial dysfunction that leads to mitophagy in exposed BECs via a mechanism unrelated to its reported genotoxicity.

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Section: Discussionmentioning

confidence: 99%

Multi-walled carbon nanotubes upregulate mitochondrial gene expression and trigger mitochondrial dysfunction in primary human bronchial epithelial cells

et al. 2019

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“…The results pointed toward the fact that the contribution of the Trojan horse mechanism toward increased cytotoxicity depends on characteristics of drugs and explains the observed differences in experimental findings by others. For example, the decrease in toxicity of bisphenol A and 17α-ethinylestradiol in the presence of CNTs could be attributed to their high lipophilicity which results in strong adsorption on CNTs resulting lower concentrations at the target site. , Similar studies have also been reported in fish and plankton whereby highly hydrophobic molecules tend to strongly bind to CNTs, resulting in decreased bioavailability, and hence decreased toxicity, of these molecules. , In contrast, very little effect of CNTs was observed on cytotoxicity of hydrophilic species such as metal ions. − Further, stronger binding of lead ions to negatively charged CNTs has been shown to reduce toxicity of lead ions compared to positively charged CNTs . Similar effects of altered toxicity of organic compounds and metal ions have also been documented with other carbonaceous nanoparticles − as well as metallic nanoparticles ,,− in diverse test systems.…”

Section: Discussionmentioning

confidence: 76%

Carbon Nanotubes Modulate Activity of Cytotoxic Compounds via a Trojan Horse Mechanism

Mehta

Kumari

Singh

2020

Chem. Res. Toxicol.

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Carbon nanotubes (CNTs) are an emerging drug delivery system, but their success is thwarted by potential toxicity concerns. In vitro and in vivo studies imply toxic potential of CNTs, but their potential to influence toxicity of coadministered compounds still remains elusive. Therefore, the present study was conducted to determine the effect of multiwalled CNTs (MWCNTs) on the toxicity of cytotoxic compounds in macrophage (RAW 264.7), lung epithelial (A549), and breast cancer (MCF-7) cell lines. The results suggest that hydrophilicity/lipophilicity of the compounds is a critical parameter. The correlation between log P and enhanced cytotoxic activity followed an inverted U-shaped curve and log P close to 1 exhibited the highest increase in cytotoxicity. Further, the increase in cytotoxicity of drug/MWCNT combinations was proportional to the degree of cellular uptake of MWCNTs. A mathematical model was developed and validated with a test set of compounds. These results suggest that MWCNTs act as a "Trojan horse" for increased intracellular delivery of drugs resulting in enhanced cytotoxic activity.

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“…However, these results suggest low toxicity of MSRs, especially short rods, at concentrations higher than those that have been used for carbon nanotubes. 84 …”

Section: Resultsmentioning

confidence: 99%

Magnetic Mesoporous Silica Nanorods Loaded with Ceria and Functionalized with Fluorophores for Multimodal Imaging

Grzelak

Gàzquez

Grayston

et al. 2022

ACS Appl. Nano Mater.

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Multifunctional magnetic nanocomposites based on mesoporous silica have a wide range of potential applications in catalysis, biomedicine, or sensing. Such particles combine responsiveness to external magnetic fields with other functionalities endowed by the agents loaded inside the pores or conjugated to the particle surface. Different applications might benefit from specific particle morphologies. In the case of biomedical applications, mesoporous silica nanospheres have been extensively studied while nanorods, with a more challenging preparation, have attracted much less attention despite the positive impact on the therapeutic performance shown by seminal studies. Here, we report on a sol–gel synthesis of mesoporous rodlike silica particles of two distinct lengths (1.4 and 0.9 μm) and aspect ratios (4.7 and 2.2) using Pluronic P123 as a structure-directing template and rendering ∼1 g of rods per batch. Iron oxide nanoparticles have been synthesized within the pores yielding maghemite (γ-Fe 2 O 3 ) nanocrystals of elongated shape (∼7 nm × 5 nm) with a [110] preferential orientation along the rod axis and a superparamagnetic character. The performance of the rods as T 2 -weighted MRI contrast agents has also been confirmed. In a subsequent step, the mesoporous silica rods were loaded with a cerium compound and their surface was functionalized with fluorophores (fluorescamine and Cyanine5) emitting at λ = 525 and 730 nm, respectively, thus highlighting the possibility of multiple imaging modalities. The biocompatibility of the rods was evaluated in vitro in a zebrafish ( Danio rerio ) liver cell line (ZFL), with results showing that neither long nor short rods with magnetic particles caused cytotoxicity in ZFL cells for concentrations up to 50 μg/ml. We advocate that such nanocomposites can find applications in medical imaging and therapy, where the influence of shape on performance can be also assessed.

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Interactions of oxidized multiwalled carbon nanotube with cadmium on zebrafish cell line: The influence of two co-exposure protocols on in vitro toxicity tests

Cited by 34 publications

References 70 publications

Multi-walled carbon nanotubes upregulate mitochondrial gene expression and trigger mitochondrial dysfunction in primary human bronchial epithelial cells

Multi-walled carbon nanotubes upregulate mitochondrial gene expression and trigger mitochondrial dysfunction in primary human bronchial epithelial cells

Carbon Nanotubes Modulate Activity of Cytotoxic Compounds via a Trojan Horse Mechanism

Magnetic Mesoporous Silica Nanorods Loaded with Ceria and Functionalized with Fluorophores for Multimodal Imaging

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