Interactions of mannose binding-lectin with red blood cells by employing cationic quantum dots

Lima, Carinna N.; Filho, Paulo E. Cabral; Santos, Beate S.; Moura, Patrícia; Fontes, Adriana

doi:10.1016/j.ijbiomac.2018.12.098

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…CdSe QDs are adsorbed into the MSP utilizing electrostatic interaction and surface-bound complexation equilibrium attractions, which correlate to the fluorescence quenching mechanisms and the UV-Vis absorption spectra results [34]. The alkyl chains of methanol molecules in CdSe QDs exhibit C-H stretching vibrations, as indicated by distinct bands at 2931 and 2855 cm −1 in Figure 8c [45,55].…”

Section: Ftir Analysis Of the Cdse Qds-msp System Interactionsmentioning

confidence: 92%

Interactions of CdSe Nanocrystals with Cationic Proteins Extracted from Moringa oleifera Seeds

Daniel,

Kapofi,

Kandawa-Schulz

et al. 2024

Photochem

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Even with significant developments in nanoscience, relatively little is known about the interactions of nanocrystal semiconducting materials with bio-macromolecules. To investigate the interfacial phenomena of cadmium selenide quantum dot (CdSe QD) nanocrystals with proteins extracted from Moringa oleifera seeds, different concentrations of cadmium selenide quantum dots–Moringa oleifera seed protein (CdSe–MSP) complexes were prepared. Respective CdSe QDs with hexagonal phase and crystalline size in the range of 4–7 nm were synthesized and labelled with the purified mesoporous MSP having a surface area of 8.4 m2/g. The interaction mechanism between CdSe QDs and MSP was studied using UV–Vis absorption, fluorescence emission and Fourier Transform Infrared spectroscopies. The UV–Vis absorption spectra showed absorption bands of CdSe–MSP complexes at 546.5 nm. The fluorescence intensity of CdSe QDs was found to decrease with increasing concentration of MSP. The thermodynamic potentials ∆Hθ (−321.3 × 103 Jmol−1); ∆Sθ (156.0 JK−1mol−1) and ∆Gθ (−46.6 × 103 Jmol−1) were also calculated. The stability of the complex found is strongly influenced by electrostatics interaction and surface-bound complexation equilibrium attraction. This information can help to elucidate the surface characteristics of MSP and its potential interactions with other molecules or nanoparticles.

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Section: Ftir Analysis Of the Cdse Qds-msp System Interactionsmentioning

confidence: 92%

Interactions of CdSe Nanocrystals with Cationic Proteins Extracted from Moringa oleifera Seeds

Daniel,

Kapofi,

Kandawa-Schulz

et al. 2024

Photochem

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“…Additionally, Mendonça and colleagues suggested that MBL deficiency contributes to vaso-occlusive events in HbSS patients [12]. More recently, it was shown that MBL can interact with healthy erythrocytes, suggesting that this lectin may be playing a membrane-modulating role in those cells [13].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, MBL usually has been studied using molecular biology techniques [29][30][31]. In cellular studies, most of the methods have been explored indirect labeling of this lectin, using antibodies conjugated with conventional fluorophores [13,32,33]. Therefore, to our knowledge, this is the first time that a conjugate constituted by QDs and MBL was prepared.…”

Section: Introductionmentioning

confidence: 99%

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Lima

Oliveira

Silva

et al. 2022

Methods Appl. Fluoresc.

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Quantum dots (QDs) have stood out as nanotools for glycobiology due to their photostability and ability to be combined with lectins. Mannose-binding lectin (MBL) is involved in the innate immune system and plays important roles in the activation of the complement cascade, opsonization, and elimination of apoptotic and microbial cells. Herein, adsorption and covalent coupling strategies were evaluated to conjugate QDs to a recombinant human MBL (rhMBL). The most efficient nanoprobe was selected by evaluating the conjugate ability to label Candida albicans yeasts by flow cytometry. The QDs-rhMBL conjugate obtained by adsorption at pH 6.0 was the most efficient, labeling ca. 100% of cells with the highest median fluorescence intensity. The conjugation was also supported by Fourier transform infrared spectroscopy, zeta potential, and size analyses. C. albicans labeling was calcium-dependent; 12% and <1% of cells were labeled in buffers without calcium and containing EDTA, respectively. The conjugate promoted specific labeling (based on cluster effect) since, after inhibition with mannan, there was a reduction of 80% in cell labeling, which did not occur with methyl-α-D-mannopyranoside monosaccharide. Conjugates maintained colloidal stability, bright fluorescence, and biological activity for at least 8 months. Therefore, QDs-rhMBL conjugates are promising nanotools to elucidate the roles of MBL in biological processes.

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Methodologies and tools to shed light on erythrophagocytosis

Turpin¹,

Meilhac

Bourdon³

et al. 2022

Biochimie

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Interactions of mannose binding-lectin with red blood cells by employing cationic quantum dots

Cited by 6 publications

References 37 publications

Interactions of CdSe Nanocrystals with Cationic Proteins Extracted from Moringa oleifera Seeds

Interactions of CdSe Nanocrystals with Cationic Proteins Extracted from Moringa oleifera Seeds

Mannose-binding lectin conjugated to quantum dots as fluorescent nanotools for carbohydrate tracing

Methodologies and tools to shed light on erythrophagocytosis

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