Interactions Between Metal Ions and DNA

Cardin, Christine J.

doi:10.1007/430_2019_42

Cited by 5 publications

(2 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S2). We also observed that ion transport classes were enriched in the transcriptome, and it is important to highlight that metal ions play a critical role in stabilizing conformations, especially in the double helix structure of DNA (Sta Cardin, 2019). Metal ions also play an important role in the MRN complex (Lee and Paull, 2005; Nicolette et al ., 2010).…”

Section: Resultsmentioning

confidence: 86%

Bacterial volatile organic compounds induce adverse ultrastructural changes and DNA damage to the sugarcane pathogenic fungus Thielaviopsis ethacetica

Freitas

Maciel

Santos

et al. 2022

Environmental Microbiology

View full text Add to dashboard Cite

Due to an increasing demand for sustainable agricultural practices, the adoption of microbial volatile organic compounds (VOCs) as antagonists against phytopathogens has emerged as an eco-friendly alternative to the use of agrochemicals. Here, we identified three Pseudomonas strains that were able to inhibit, in vitro, up to 80% of mycelial growth of the phytopathogenic fungus Thielaviopsis ethacetica, the causal agent of pineapple sett rot disease in sugarcane. Using GC/MS, we found that these bacteria produced 62 different VOCs, and further functional validation revealed compounds with high antagonistic activity to T. ethacetica. Transcriptomic analysis of the fungal response to VOCs indicated that these metabolites downregulated genes related to fungal central metabolism, such as those involved in carbohydrate metabolism. Interestingly, genes related to the DNA damage response were upregulated, and micro-FTIR analysis corroborated our hypothesis that VOCs triggered DNA damage. Electron microscopy analysis showed critical morphological changes in mycelia treated with VOCs. Altogether, these results indicated that VOCs hampered fungal growth and could lead to cell death. This study represents the first demonstration of the molecular mechanisms involved in the antagonism of sugarcane phytopathogens by VOCs and reinforces that VOCs can be a sustainable alternative for use in phytopathogen biocontrol.

show abstract

Section: Resultsmentioning

confidence: 86%

Bacterial volatile organic compounds induce adverse ultrastructural changes and DNA damage to the sugarcane pathogenic fungus Thielaviopsis ethacetica

Freitas

Maciel

Santos

et al. 2022

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…The conformational differences between furanose rings in RNA and DNA may be a manifestation of polymeric structures because nucleosides and nucleotides may crystallize in either of these two conformations. However, the degree of hydration and metal-ion coordination can significantly affect the conformation of the furanose ring; e.g., A-DNA (75% relative humidity, sodium salt) exhibits a C3′- endo conformation, whereas B-DNA (95% humidity, lithium salt) favors the C2′- endo form . First, the puckering amplitude (τ m ) and phase angle of pseudorotation ( P ) were defined by Altona and Sundaralingam in 1972 when they introduced the concept of pseudorotation to describe the puckering motion of the furanose ring in nucleosides and nucleotides .…”

Section: Introductionmentioning

confidence: 99%

Conformation Locking of the Pentose Ring in Nucleotide Monophosphate Coordination Polymers via π–π Stacking and Metal-Ion Coordination

Zhu

Song

et al. 2021

Inorg. Chem.

View full text Add to dashboard Cite

The conformation of the pentose ring in nucleotides is extremely important and a basic problem in biochemistry and pharmaceutical chemistry. In this study, we used a strategy to regulate the conformation of pentose rings of nucleotides via the synergistic effect of metal-ion coordination and π–π stacking. Seven types of coordination complexes were developed and characterized using Fourier transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, powder X-ray diffraction, ultraviolet–visible spectroscopy, 1H nuclear magnetic resonance spectroscopy, electrospray ionization mass spectrometry, and single-crystal X-ray diffraction. On the basis of two conformational parameters obtained from single-crystal structure analysis, i.e., the pseudorotation phase angle and degree of puckering, the exact conformation of the furanose ring in these coordination polymers was unequivocally determined. Crystallographic studies demonstrate that a short bridging ligand (4,4′-bipyridine) is conducive to the formation of a twist form, and long auxiliary ligands [1,2-bis(4-pyridyl)ethene and 4,4′-azopyridine] induce the formation of an envelope conformation. However, the longest auxiliary ligands [1,4-bis(4-pyridyl)-2,3-diaza-1,3-butadiene] cannot limit the flexibility of a nucleotide. Our results demonstrated that the proposed strategy is universal and controllable. Moreover, the chirality of these coordination polymers was examined by combining the explanation of their crystal structures with solid-state circular dichroism spectroscopy measurements.

show abstract

Polypyridyl‐Based Copper Phenanthrene Complexes: Combining Stability with Enhanced DNA Recognition

Fantoni

Molphy

O'Carroll

et al. 2020

Chemistry A European J

View full text Add to dashboard Cite

We report a series of copper(II) artificial metallo‐nucleases (AMNs) and demonstrate their DNA damaging properties and in‐vitro cytotoxicity against human‐derived pancreatic cancer cells. The compounds combine a tris‐chelating polypyridyl ligand, di‐(2‐pycolyl)amine (DPA), and a DNA intercalating phenanthrene unit. Their general formula is Cu‐DPA‐N,N' (where N,N'=1,10‐phenanthroline (Phen), dipyridoquinoxaline (DPQ) or dipyridophenazine (DPPZ)). Characterisation was achieved by X‐ray crystallography and continuous‐wave EPR (cw‐EPR), hyperfine sublevel correlation (HYSCORE) and Davies electron‐nuclear double resonance (ENDOR) spectroscopies. The presence of the DPA ligand enhances solution stability and facilitates enhanced DNA recognition with apparent binding constants (Kapp) rising from 105 to 107 m−1 with increasing extent of planar phenanthrene. Cu‐DPA‐DPPZ, the complex with greatest DNA binding and intercalation effects, recognises the minor groove of guanine–cytosine (G‐C) rich sequences. Oxidative DNA damage also occurs in the minor groove and can be inhibited by superoxide and hydroxyl radical trapping agents. The complexes, particularly Cu‐DPA‐DPPZ, display promising anticancer activity against human pancreatic tumour cells with in‐vitro results surpassing the clinical platinum(II) drug oxaliplatin.

show abstract

Interactions Between Metal Ions and DNA

Cited by 5 publications

References 95 publications

Bacterial volatile organic compounds induce adverse ultrastructural changes and DNA damage to the sugarcane pathogenic fungus Thielaviopsis ethacetica

Bacterial volatile organic compounds induce adverse ultrastructural changes and DNA damage to the sugarcane pathogenic fungus Thielaviopsis ethacetica

Conformation Locking of the Pentose Ring in Nucleotide Monophosphate Coordination Polymers via π–π Stacking and Metal-Ion Coordination

Polypyridyl‐Based Copper Phenanthrene Complexes: Combining Stability with Enhanced DNA Recognition

Contact Info

Product

Resources

About