1978
DOI: 10.1111/j.1432-1033.1978.tb12209.x
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Membrane fragments rich in cholinergic (nicotinic) receptor protein were purified from the electric organ of Torpedo marmoruta. Their lipid composition is essentially characterized by the prominence of cholesterol, phosphatidylethanolamine and phosphatidylcholine, long-chain fatty acyl constituents, and the absence of sphingomyelin. Solubilised receptor was purified from these fragments and the concentration of sodium cholate lowered by dialysis to 0.01 76 (w/v). When this preparation was injected under a lipi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

4
64
1

Year Published

1978
1978
2007
2007

Publication Types

Select...
5
1
1

Relationship

2
5

Authors

Journals

citations
Cited by 113 publications
(69 citation statements)
references
References 64 publications
(40 reference statements)
4
64
1
Order By: Relevance
“…lipid complexes thus obtained have lipid : protein ratio of 9 : 1 w/w, which is rather different from that of the highly purified receptorrich membrane fragments (approx. 1 :2) [24]. These lipoprotein complexes form vesicles where equal numbers of a-toxin sites face the interior and the exterior of the vesicles.…”
Section: Discussionmentioning
confidence: 99%
“…lipid complexes thus obtained have lipid : protein ratio of 9 : 1 w/w, which is rather different from that of the highly purified receptorrich membrane fragments (approx. 1 :2) [24]. These lipoprotein complexes form vesicles where equal numbers of a-toxin sites face the interior and the exterior of the vesicles.…”
Section: Discussionmentioning
confidence: 99%
“…Under the standard conditions of reconstitution from a concentrated sodium cholate extract (see above), endogenous phospholipids were present in the soluble extract at a concentration higher than about 0.25 %, wjv (as estimated by phosphorus determination and the value for the average molecular weight of the phospholipids for Torpedo membranes in [39]), i.e. at a much higher value than the critical concentration found for the conservation of the allosteric properties of the receptor in its soluble form with 0.2% (w/v) asolectin, and were centrifuged for 14 h at 36000 rev./min in a Beckman SW 41 rotor; ( 0 0 ) a-'251-bungarotoxinlabelled acetylcholine receptor; (-0) [3H]phosphatidylcholine added in trace amounts to the soluble extract before centrifugation.…”
Section: Methodological Requirements For the Reconstitution Of A Funcmentioning
confidence: 99%
“…(a) as expected from the localisation and integration of the receptor protein in the membrane bilayer [41,65,661, interactions between lipid molecules and the membrane-bound protein [75] and even the purified protein [36,39,76] have been demonstrated; (b) any treatment which results in the delipidation of the receptor protein (see Results and [36]) also results in the loss of the characteristic binding properties of the protein; (c) addition of exogenous lipids to the soluble cholate receptor extract, or simply the maintenance of a high concentration of the endogenous lipids prevent this loss.…”
Section: Sodium Cliolute Und Lipids Us 'Allostuic' Ejjectors Of the Cmentioning
confidence: 99%
See 2 more Smart Citations