Interaction of synthetic antimicrobial peptides of the Hylin a1 family with models of eukaryotic structures: Zwitterionic membranes and DNA

Muniz, Gabriel S. Vignoli; Torre, Lilia I. De la; Duarte, Evandro L.; Lorenzón, Esteban Nicolás; Cilli, Eduardo Maffud; Balan, Andrea; Lamy, M. Teresa

doi:10.1016/j.bbrep.2020.100827

Cited by 4 publications

(6 citation statements)

References 50 publications

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“…Carboxyfluorescein leakage assay is a powerful method to study pore formation on lipid vesicles caused by exogenous molecules 36 , 37 . Briefly, the fluorophore carboxyfluorescein (CF) at high concentration, encapsulated in liposomes, displays no fluorescence due to self-quenching.…”

Section: Resultsmentioning

confidence: 99%

“…The percentage of CF leakage, (%) leakage, was determined according to: where I ( t ) is the fluorescence intensity at time t , I 0 is the initial fluorescence, before CTX addition, and I total is the maximum fluorescence obtained after the addition of Triton X-100 36 , 37 , 60 .…”

Section: Methodsmentioning

confidence: 99%

“…Kinetic analysis was performed with vesicles of DPPC, DPPG and DPPS, without and with addition of 0.1 mol% of CTX, in the lipid gel phase only (at 25 °C). The experimental procedure in the fluid phase for these lipids (at 50 °C) was considered unreliable, since a considerable leakage of CF was detected for pure lipid vesicles 36 , 37 . Hence, in order to mimic the fluid phase of the dipalmitoyl membranes, similarly prepared vesicles of POPC, POPG and POPS where used, without and with addition of CTX, at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

“…where I(t) is the fluorescence intensity at time t, I 0 is the initial fluorescence, before CTX addition, and I total is the maximum fluorescence obtained after the addition of Triton X-100 36,37,60 . Kinetic analysis was performed with vesicles of DPPC, DPPG and DPPS, without and with addition of 0.1 mol% of CTX, in the lipid gel phase only (at 25 °C).…”

Section: Esr Spectral Simulationsmentioning

confidence: 99%

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Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes

Pimenta

Duarte

Muniz

et al. 2021

Sci Rep

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The important pharmacological actions of Crotoxin (CTX) on macrophages, the main toxin in the venom of Crotalus durissus terrificus, and its important participation in the control of different pathophysiological processes, have been demonstrated. The biological activities performed by macrophages are related to signaling mediated by receptors expressed on the membrane surface of these cells or opening and closing of ion channels, generation of membrane curvature and pore formation. In the present work, the interaction of the CTX complex with the cell membrane of macrophages is studied, both using biological cells and synthetic lipid membranes to monitor structural alterations induced by the protein. Here we show that CTX can penetrate THP-1 cells and induce pores only in anionic lipid model membranes, suggesting that a possible access pathway for CTX to the cell is via lipids with anionic polar heads. Considering that the selectivity of the lipid composition varies in different tissues and organs of the human body, the thermostructural studies presented here are extremely important to open new investigations on the biological activities of CTX in different biological systems.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Esr Spectral Simulationsmentioning

confidence: 99%

See 2 more Smart Citations

Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes

Pimenta

Duarte

Muniz

et al. 2021

Sci Rep

Self Cite

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“…These results can be explained by the low absorption of this antimicrobial agent by the C. albicans biofilm matrix. Although peptides from the Hylin−a1 family have been shown to interact with lipid bilayers of bacteria biofilms [ 45 ], the Hylin−a1 net charge may not have been able to penetrate the C. albicans biofilm multilayer and reach the target cell [ 26 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans

Dias,

Cilli,

Medeiros

et al. 2024

Microorganisms

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The aim of the study was to investigate the effect of antimicrobial peptides (AMPs) Hylin−a1, KR−12-a5, and Temporin-SHa in Candida albicans as well as the biocompatibility of keratinocytes spontaneously immortalized (NOK-si) and human gingival fibroblasts (FGH) cells. Initially, the susceptible (CaS—ATCC 90028) and fluconazole-resistant (CaR—ATCC 96901) C. albicans strains were grown to evaluate the effect of each AMP in planktonic culture, biofilm, and biocompatibility on oral cells. Among the AMPs evaluated, temporin−SHa showed the most promising results. After 24 h of Temporin-SHa exposure, the survival curve results showed that CaS and CaR suspensions reduced 72% and 70% of cell viability compared to the control group. The minimum inhibitory/fungicide concentrations (MIC and MFC) showed that Temporin−SHa was able to reduce ≥50% at ≥256 µg/mL for both strains. The inhibition of biofilm formation, efficacy against biofilm formation, and total biomass assays were performed until 48 h of biofilm maturation, and Temporin-SHa was able to reduce ≥50% of CaS and CaR growth. Furthermore, Temporin−SHa (512 µg/mL) was classified as non-cytotoxic and slightly cytotoxic for NOK-si and FGH, respectively. Temporin−SHa demonstrated an anti-biofilm effect against CaS and CaR and was biocompatible with NOK-si and FGH oral cells in monolayer.

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What different physical techniques can disclose about disruptions on membrane structure caused by the antimicrobial peptide Hylin a1 and a more positively charged analogue

Muniz

Duarte

Lorenzón

et al. 2022

Chemistry and Physics of Lipids

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Interaction of synthetic antimicrobial peptides of the Hylin a1 family with models of eukaryotic structures: Zwitterionic membranes and DNA

Cited by 4 publications

References 50 publications

Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes

Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes

Antibiofilm Activity and Biocompatibility of Temporin-SHa: A Promising Antimicrobial Peptide for Control of Fluconazole-Resistant Candida albicans

What different physical techniques can disclose about disruptions on membrane structure caused by the antimicrobial peptide Hylin a1 and a more positively charged analogue

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