Interaction of Exercise Training and Chronic Ethanol Ingestion on Hepatic and Plasma Antioxidant System in Rat

Abstract

This study was undertaken in order to investigate the interactive effects of exercise training and chronic ethanol consumption on the antioxidant system in rat liver and plasma. Fisher‐344 rats were treated in separate groups as follows: sedentary control (SC); exercise training (ET) for 6.5 weeks; ethanol 20% (2.0 g kg−1, p.o.) for 6.5 weeks; and ET and ethanol administration. In liver, ET significantly decreased the malondialdehyde (MDA) level (73% of SC). Chronic ethanol significantly increased catalase (CAT) activity and MDA levels (126% and 135% of SC), respectively, and also depleted the reduced glutathione (GSH) level and the reduced to oxidized glutathione (GSH/GSSG) ratio (81% and 38% of SC), respectively. Exercise training plus ethanol significantly increased CAT and glutathione reductase (GR) activity (126% and 118% of SC), respectively, and decreased the MDA level (67% of SC). In plasma, ethanol significantly enhanced CAT activity and MDA levels (173% and 221% of SC), respectively. Ethanol ingestion also increased the CAT/superoxide dismutase (SOD) ratio (216% of SC) in plasma. Training plus ethanol ingestion significantly increased CAT activity and MDA levels (208% and 148% of SC), respectively, and increased CAT/SOD and glutathione peroxidase (GSH‐Px)/SOD ratios (279% and 142% of SC), respectively. The data indicate that the combination of exercise and ethanol ingestion resulted in an enhanced hepatic CAT and GR activity to eliminate H2O2 and to maintain endogenous GSH levels. Thus, training ameliorated the ethanol‐induced oxidative injury in the liver. The ratio of CAT/SOD in plasma increased twofold due to chronic ethanol intake and threefold due to the combination, which may be used as an index of oxidative stress. © 1997 John Wiley & Sons, Ltd.

The pharmacological potential of Sorbus commixta cortex on blood alcohol concentration and hepatic lipid peroxidation in acute alcohol-treated rats

“…In several previous studies, it has been also reported that catalase activity was reduced in acute alcohol-treated rats, suggesting that the reduced activity was due to oxidative inactivation by ROS generated from alcohol metabolism or inhibition by elevated influx of superoxides, a catalase inhibitor (Pigeolet et al 1990;Schlorff et al 1999). Our results are consistent with the findings of Bykova & Zhukova (1991), Dinu & Zamfir (1991) and Husain & Somani (1997), who reported decreased catalase activity in the liver of alcohol-treated rats. Based on these findings, catalase appears to be used as an antioxidant defence enzyme to catalyse H 2 O 2 decomposition rather than as an alcohol oxidizing enzyme in this experiment as well.…”

Activation of opioid μ-receptors by loperamide to improve interleukin-6-induced inhibition of insulin signals in myoblast C2C12 cells

“…Both reactive oxygen species [21] and lipopolysaccharide [22] can increase IL-6 in skeletal muscle. Exercise alters reactive oxygen species activities in a variety of different ways [23,24]. Such variable responses to exercise have also been observed in different species [25,26], and we observed that IL-6 inhibits insulin action to induce insulin resistance in C 2 C 12 cells.…”

Increase of Anti‐oxidation by Exercise in the Liver of Obese Zucker Rats

“…Actually, exercise has been indicated to alter anti-oxidant enzyme tissue activities in a variety of different ways depending on the type of exercise programme used, the amount of exercise and the existence of a resting period between the exercise regimen and tissue collection. 23,24 Such variable responses to exercise have also been observed in different species. 25,26 Our results are consistent with the previous view that training adaptation occurs only for Mn-SOD but not for Cu/Zn-SOD.…”