Interaction of cationic liposomes with cell membrane models

Bonicelli, M.G.; Giansanti, Luisa; Ierino, Marco; Mancini, Giovanna

doi:10.1016/j.jcis.2010.12.005

Cited by 15 publications

(9 citation statements)

References 14 publications

(9 reference statements)

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“…Lipoid E PC S (from egg; phosphatidylcholine content ≥ 96%) (EPC) and egg phosphatidylglycerol (EPG) were generous gifts from Lipoid GmbH (Ludwigshafen, Germany), while AZT in the form of a dihydrate was kindly donated by PLIVA Croatia Ltd. (Zagreb, Croatia). Dimethyldioctadecylammonium bromide (DODAB) and polyoxyethylene (20) sorbitanmonooleate (Tween 80) were provided by Sigma-Aldrich (St. Louis, MO, USA). Ethanol and KH 2 PO 4 were purchased from Kemika (Zagreb, Croatia).…”

Section: Methodsmentioning

confidence: 99%

“…These results are in accordance with the previous findings for deformable liposomes [25], and are considered beneficial regarding physical stability of the AZT-liposomes. Bilayers of all the liposomes consisted of 20% non-ionic single chain surfactant, i.e., polyoxyethylene (20) sorbitan monooleate (Tween 80), which has already been reported as the edge activator in deformable (elastic) liposomes [31]. The edge activator destabilizes the tight packing of phospholipids in the liposomal bilayers, making them deformable (flexible) and squeezable, thus subsequently enhancing permeation of the vesicles and/or encapsulated drug into/through the skin and cervicovaginal tissue [18,19,32].…”

Section: Physico-chemical Properties Of the Azt-liposomesmentioning

confidence: 99%

“…Our hypothesis was that by facilitating interaction of the liposomes with the C. trachomatis infected HeLa cells increased intracellular AZT delivery could be obtained. Namely, cationic liposomes have been demonstrated to contribute interaction with the negatively surface charged cell membranes [20] as well as bacteria [13,14], and significantly increase the activity of encapsulated drugs against planktonic [21] and biofilm-forming bacteria [10].…”

Section: Introductionmentioning

confidence: 99%

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Liposomal Encapsulation Increases the Efficacy of Azithromycin against Chlamydia trachomatis

et al. 2021

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Chlamydia trachomatis (C. trachomatis) is an obligate intracellular bacterium linked to ocular and urogenital infections with potentially serious sequelae, including blindness and infertility. First-line antibiotics, such as azithromycin (AZT) and doxycycline, are effective, but treatment failures have also been reported. Encapsulation of antibiotics in liposomes is considered an effective approach for improving their local effects, bioavailability, biocompatibility and antimicrobial activity. To test whether liposomes could enhance the antichlamydial action of AZT, we encapsulated AZT in different surface-charged elastic liposomes (neutral, cationic and anionic elastic liposomes) and assessed their antibacterial potential against the C. trachomatis serovar D laboratory strain as well as the clinical isolate C. trachomatis serovar F. A direct quantitative polymerase chain reaction (qPCR) method was used to measure chlamydial genome content 48 h post infection and to determine the recoverable chlamydial growth. All the liposomes efficiently delivered AZT to HeLa 229 cells infected with the laboratory Chlamydia strain, exhibiting the minimal inhibitory concentrations (MIC) and the minimal bactericidal concentrations (MBC) of AZT even 4–8-fold lower than those achieved with the free AZT. The tested AZT-liposomes were also effective against the clinical Chlamydia strain by decreasing MIC values by 2-fold relative to the free AZT. Interestingly, the neutral AZT-liposomes had no effect on the MBC against the clinical strain, while cationic and anionic AZT-liposomes decreased the MBC 2-fold, hence proving the potential of the surface-charged elastic liposomes to improve the effectiveness of AZT against C. trachomatis.

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Section: Methodsmentioning

confidence: 99%

Section: Physico-chemical Properties Of the Azt-liposomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Liposomal Encapsulation Increases the Efficacy of Azithromycin against Chlamydia trachomatis

et al. 2021

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“…Liposomes offer several advantages over other delivery systems, including biocompatibility, control of biological properties via modification of the physicochemical properties, such as lipid composition (in particular, the presence of cholesterol and stearylamine), vesicle size, lamellarity, positive surface charge and lipid membrane fluidity. All these are important characteristics that modulate the therapeutic role and interaction with cells by several mechanisms of drug delivery [ 41 , 87 , 89 , 98 , 118 , 119 , 120 , 121 ].…”

Section: Recent Advances In Liposomes Technologymentioning

confidence: 99%

Mechanisms and Pharmaceutical Action of Lipid Nanoformulation of Natural Bioactive Compounds as Efficient Delivery Systems in the Therapy of Osteoarthritis

et al. 2021

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Osteoarthritis (OA) is a degenerative joint disease. An objective of the nanomedicine and drug delivery systems field is to design suitable pharmaceutical nanocarriers with controllable properties for drug delivery and site-specific targeting, in order to achieve greater efficacy and minimal toxicity, compared to the conventional drugs. The aim of this review is to present recent data on natural bioactive compounds with anti-inflammatory properties and efficacy in the treatment of OA, their formulation in lipid nanostructured carriers, mainly liposomes, as controlled release systems and the possibility to be intra-articularly (IA) administered. The literature regarding glycosaminoglycans, proteins, polyphenols and their ability to modify the cell response and mechanisms of action in different models of inflammation are reviewed. The advantages and limits of using lipid nanoformulations as drug delivery systems in OA treatment and the suitable route of administration are also discussed. Liposomes containing glycosaminoglycans presented good biocompatibility, lack of immune system activation, targeted delivery of bioactive compounds to the site of action, protection and efficiency of the encapsulated material, and prolonged duration of action, being highly recommended as controlled delivery systems in OA therapy through IA administration. Lipid nanoformulations of polyphenols were tested both in vivo and in vitro models that mimic OA conditions after IA or other routes of administration, recommending their clinical application.

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“…Pulmonary administration is a method that works via the lungs. Among the several types of drug carriers, including liposomes [4,5], micelles [6][7][8][9], and gels [10][11][12], polymeric "particles" are suitable for pulmonary drug administration [13]. In this method, the drug-incorporated particle is aspirated into the lung and decomposed via transcytosis, and then the drug is absorbed into the body.…”

Section: Introductionmentioning

confidence: 99%

One-step synthesis of polyethylene microspheres using a modified chemical route for pulmonary drug delivery

Deshmukh¹,

Sangawar

2016

Journal of Taibah University for Science

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Polyethylene microspheres (microparticles) were prepared using a modified chemical route. The prepared powder samples were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The scanning electron microscopy images show that the concentration of polyglycolic acid decreased the agglomeration and increased the degree of sphericity of the polyethylene microspheres. The results show that the polyethylene microparticles may be good candidates as drug carriers for pulmonary drug delivery.

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Interaction of cationic liposomes with cell membrane models

Cited by 15 publications

References 14 publications

Liposomal Encapsulation Increases the Efficacy of Azithromycin against Chlamydia trachomatis

Liposomal Encapsulation Increases the Efficacy of Azithromycin against Chlamydia trachomatis

Mechanisms and Pharmaceutical Action of Lipid Nanoformulation of Natural Bioactive Compounds as Efficient Delivery Systems in the Therapy of Osteoarthritis

One-step synthesis of polyethylene microspheres using a modified chemical route for pulmonary drug delivery

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