Interaction of Bovine Serum Albumin with Chrysotile: Spectroscopic and Morphological Studies

Abstract

The biodurability of chrysotile fibers, which is related to their cytotoxicity and mutagenic responses, is strongly affected by the surface chemical adsorption of biological molecules. Natural chrysotile is a heterogeneous material in both structure and composition. The availability of synthetic stoichiometric chrysotile of constant structure and uniform morphology has allowed us to investigate its interaction with bovine serum albumin (BSA). By using transmission electron microscopy (TEM) and atomic force microscopy (AFM), we have obtained the first morphological evidence of albumin adsorption onto chrysotile nanocrystals. FTIR spectroscopy was used to quantify modifications of BSA secondary structure that were induced by the surface interaction. The protein transition to beta-turns allows a stronger interaction between the protein hydrophilic side-chains and the charged asbestos surface, which is consistent with hydrogen bonds involving the superficial OH groups. Synthetic stoichiometric chrysotile nanocrystals were shown to be an ideal reference standard with which to study the interaction of asbestos fibers with biological systems, in order to elucidate the chemical mechanisms of asbestos toxicity.

Adsorbents for acetone in cyclohexane effuent employed in Ziegler-Natta catalyst process

“…4, some tubular structure can be observed, indicating that the fibrous morphology was kept after leaching. Similar images have been reported in the literature (Wypych et al 2005;Halma et al 2006;Falini et al 2006;). …”

“…The peak at 1090 cm −1 is attributed to the vibration modes of Si-O network that consists of Si-O-Si and O-Si-O, and the Si-O stretching peak which is vertical to the prolongation of the fiber, was undetected at 1024 cm −1 due to natural chrysotile tetrahedral sheets attacked by HCl. The peak at 617 cm −1 was identified as the terminal Si-OH deformation band, the Si-OSi bending peak was detected at 465 cm −1 (Wypych et al 2005;Wang et al 2006;Halma et al 2006;Falini et al 2006).…”

Adsorption of bovine serum albumin onto synthetic Fe-doped geomimetic chrysotile

“…The geoinspired synthetic chrysotile fibres with stoichiometric composition Mg 3 Si 2 O 5 (OH) 4 , in contrast to the natural ones, do not exert any significant cytotoxic effect [15]. Moreover, the interaction of this synthetic chrysotile with bovine serum albumin (BSA) and human serum albumin (HSA) has been investigated [16][17][18].…”

“…From the second derivative of the bands, the actual half-width can be estimated, based on which a curve-fitting process was iterated to achieve the best fitted curves. The component bands were assigned on the basis of the literature data [16,[28][29][30][31][32][33].…”

Quantitative structure-property relationship study on the determination of binding constant by fluorescence quenching

“…For a series of studies concerning the interaction between drugs and protein, it is necessary to determinate the respective binding constant (K). So far, many studies have been carried out to investigate the interaction of proteins with drugs by fluorescence spectroscopy [5][6][7], FTIR [8,9] and CD spectroscopy [10,11]. Fluorescence techniques are one of the powerful tools in the study of interactions between drugs and plasma proteins in general and serum albumin in particular because of their high sensitivity, rapidity and ease of implementation.…”