Interaction between MTHFR 677C&gt;T, PON1 192Q&gt;R and PON1 55L&gt;M polymorphisms and its effect on non-recurrent spontaneous abortion in Mexican women

Blanco-Muñoz, Julia; Lacasaña, Marina; Gamboa, Ricardo; Huesca-Gómez, Claudia; Hernández-Mariano, José Ángel; Aguilar-Garduño, Clemente

doi:10.1016/j.gene.2018.11.093

Cited by 3 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research showed that FA supplementation continued throughout pregnancy prevents adverse pregnancy outcomes [1]. MTHFR is a key enzyme in folate metabolism [23]. Some genetic polymorphisms code for a less e cient enzyme, increasing serum concentrations of homocysteine [23].…”

Section: Discussionmentioning

confidence: 99%

“…MTHFR is a key enzyme in folate metabolism [23]. Some genetic polymorphisms code for a less e cient enzyme, increasing serum concentrations of homocysteine [23]. This has been associated with inadequate feto-maternal circulation and increased risk of adverse pregnancy outcome [14,23].…”

Section: Discussionmentioning

confidence: 99%

“…Some genetic polymorphisms code for a less e cient enzyme, increasing serum concentrations of homocysteine [23]. This has been associated with inadequate feto-maternal circulation and increased risk of adverse pregnancy outcome [14,23]. Two polymorphic variants in this gene (C677T and A1298C) have been implicated in a mild form of MTHFR de ciency associated with hyperhomocysteinemia [24].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Individualized Folic Acid Supplementation based on Polymorphisms of Methylenetetrahydrofolate Reductase (MTHFR) and Methionine Synthase Reductase (MTRR), Compared with Traditional Folic Acid Supplementation, Reduces Gestational Diabetes Mellitus

Diao

et al. 2022

Preprint

View full text Add to dashboard Cite

Backgroud: Folic Acid (FA) may contribute to the development of gestational diabetes mellitus (GDM), but existing studies are inconsistent. We examined the genotype distributions and allele frequencies of methylenetetrahydrofolate reductase (MTHFR) C677T, A1298C and methionine synthase reductase (MTRR) A66G polymorphisms of pregnant women in China, and compared the effects of individualized folate supplementation and traditional FA supplementation on GDM.Methods: The genotype distributions and allele frequencies of MTHFR C677T, A1298C and MTRR A66G polymorphisms in 968 pregnant women (case group) were tested. FA metabolism was ranked at four levels, and then pregnant women of different levels are supplemented with different doses of FA at different periods. The case group was followed up for pregnancy complications and compared with 1,940 pregnant women traditionally supplemented with FA in the same hospital (control group).Results: The allele frequencies of MTHFR C677T were 63.3% (C) and 36.7% (T), those of MTHFR A1298C were 79.3% (A) and 20.7% (C), and those of MTRR A66G were 75.0% (A) and 25.0% (G). Compared with control group, the incidence of GDM in the case group were significantly lower, especially in high-risk pregnant women after FA supplementation.Conclusion: Traditional FA supplementation based on personal habits is controversial, but the use of polymorphisms of genes to clarify the FA metabolism of pregnant women, appropriate, timely and accurate supplementation of FA can effectively reduce gestational diabetes, especially for high-risk pregnant women.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Individualized Folic Acid Supplementation based on Polymorphisms of Methylenetetrahydrofolate Reductase (MTHFR) and Methionine Synthase Reductase (MTRR), Compared with Traditional Folic Acid Supplementation, Reduces Gestational Diabetes Mellitus

Diao

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Indeed, it is widely distributed among tissues, such as liver, kidney, intestine and serum, and can hydrolyze a variety of substrates [ 1 , 2 ]. PON1 is biosynthesized mainly in the liver and secreted into the blood where it associates predominantly with high-density lipoprotein (HDL) [ 3 , 4 , 5 , 6 ], due to a hydrophobic signal sequence in its N-terminal region [ 7 ]. PON1 isoforms are known to have a broad range of roles including being involved in mediating against cardiovascular diseases, drug metabolism and bacterial infections [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

The Hydrolysis Rate of Paraoxonase-1 Q and R Isoenzymes: An In Silico Study Based on In Vitro Data

et al. 2022

View full text Add to dashboard Cite

Human serum paraoxonase-1 (PON1) is an important hydrolase-type enzyme found in numerous tissues. Notably, it can exist in two isozyme-forms, Q and R, that exhibit different activities. This study presents an in silico (QSAR, Docking, MD and QM/MM) study of a set of compounds on the activity towards the PON1 isoenzymes (QPON1 and RPON1). Different rates of reaction for the Q and R isoenzymes were analyzed by modelling the effect of Q192R mutation on active sites. It was concluded that the Q192R mutation is not even close to the active site, while it is still changing the geometry of it. Using the combined genetic algorithm with multiple linear regression (GA-MLR) technique, several QSAR models were developed and relative activity rates of the isozymes of PON1 explained. From these, two QSAR models were selected, one each for the QPON1 and RPON1. Best selected models are four-variable MLR models for both Q and R isozymes with squared correlation coefficient R2 values of 0.87 and 0.83, respectively. In addition, the applicability domain of the models was analyzed based on the Williams plot. The results were discussed in the light of the main factors that influence the hydrolysis activity of the PON1 isozymes.

show abstract

“…PON1 has two significant encoding polymorphisms like Met/Leu55 and Gln/Arg192 . The Met/Leu55 coding polymorphism has been associated with some pathophysiological conditions such as CADs, Parkinson’s disease, stroke, changes in plasma levels of total and LDL .…”

Section: Introductionmentioning

confidence: 99%

A potential risk factor for paraoxonase 1: in silico and in-vitro analysis of the biological activity of proton-pump inhibitors†

Türkeş

2019

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

Objectives Proton‐pump inhibitors (PPIs) are drugs commonly utilized by about 7% of adults in the world. Recent researches have shown that there are countless and severe side effects of these drugs. This situation has raised concern among clinicians and patients alike. The purpose of this study is to contribute the novel drug discovery and development technology and toxicology field by researching interactions of PPIs on paraoxonase 1. Methods In this study, the paraoxonase 1 enzyme was purified from human serum by using rapid and straightforward chromatographic techniques. Subsequently, the inhibition effects of pantoprazole, omeprazole, and esomeprazole, PPIs, were investigated on paraoxonase 1. Besides, molecular docking studies were performed to unravel the binding mechanism between the enzyme and drugs. Key findings All drugs showed potent inhibitory activities. IC50 of the drugs values were 54.780 ± 0.524, 86.470 ± 0.818 and 93.390 ± 0.885 mm and Ki constants were found as 39.895 ± 0.005 mm, 70.112 ± 0.010 mm and 78.868 ± 0.008 mm, respectively. The binding scores observed in silico studies were found to agree with the obtained from in‐vitro experimental results. Conclusions We observed that the drugs decreased PON1 activity at low concentrations. The results show that adjusting the dosages of these medications is a crucial case for each patient. The physicians should more carefully interpret whether there is an essential indication before prescribing PPIs and, if there is, to approve the proper dosing for the situation.

show abstract

Interaction between MTHFR 677C>T, PON1 192Q>R and PON1 55L>M polymorphisms and its effect on non-recurrent spontaneous abortion in Mexican women

Cited by 3 publications

References 38 publications

Individualized Folic Acid Supplementation based on Polymorphisms of Methylenetetrahydrofolate Reductase (MTHFR) and Methionine Synthase Reductase (MTRR), Compared with Traditional Folic Acid Supplementation, Reduces Gestational Diabetes Mellitus

Individualized Folic Acid Supplementation based on Polymorphisms of Methylenetetrahydrofolate Reductase (MTHFR) and Methionine Synthase Reductase (MTRR), Compared with Traditional Folic Acid Supplementation, Reduces Gestational Diabetes Mellitus

The Hydrolysis Rate of Paraoxonase-1 Q and R Isoenzymes: An In Silico Study Based on In Vitro Data

A potential risk factor for paraoxonase 1: in silico and in-vitro analysis of the biological activity of proton-pump inhibitors†

Contact Info

Product

Resources

About