Interaction between human mucins and parasite glycoproteins: the role of lectins and glycosidases in colonization by intestinal protozoa

Martinez-Ocaña, Joel; Maravilla, Pablo; Olivo-Dı́az, Angélica

doi:10.1590/s1678-9946202062064

Cited by 10 publications

(10 citation statements)

References 61 publications

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“…Mucins have been reported both in the host and in some gastrointestinal parasites. Cryptosporidium ’s mucins are important for attachment and invasion to the host cell ( 55 ) or evasion of the host immune system ( 89 ). Many essential Cryptosporidium proteins are mucin-like proteins such as gp40/15 ( 90 ), cpClec, gp30, and gp900 ( 91 ).…”

Section: Discussionmentioning

confidence: 99%

Dual Transcriptomics To Determine Gamma Interferon-Independent Host Response to Intestinal Cryptosporidium parvum Infection

et al. 2022

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Animals with a chronic infection of the parasite Toxoplasma gondii are protected against lethal secondary infection with other pathogens. Our group previously determined that soluble T. gondii antigens (STAg) can mimic this protection and be used as a treatment against several lethal pathogens. Because treatments are limited for the parasite Cryptosporidium parvum , we tested STAg as a C. parvum therapeutic. We determined that STAg treatment reduced C. parvum Iowa II oocyst shedding in IFNγ-KO mice. Murine intestinal sections were then sequenced to define the IFNγ independent transcriptomic response to C. parvum infection. Gene Ontology and transcript abundance comparisons showed host immune response and metabolism changes. Transcripts for type I interferon responsive genes were more abundant in C. parvum infected mice treated with STAg. Comparisons between PBS or STAg treatments showed no significant differences in C. parvum gene expression. C. parvum transcript abundance was highest in the ileum and mucin-like glycoproteins and the GDP-fucose transporter were among the most abundant. These results will assist the field in determining both host- and parasite-directed future therapeutic targets.

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Section: Discussionmentioning

confidence: 99%

Dual Transcriptomics To Determine Gamma Interferon-Independent Host Response to Intestinal Cryptosporidium parvum Infection

et al. 2022

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“…INS-15, nevertheless, appears to be a dense granule protein present over the entire merozoites of C. parvum ( Figure 4 B). The only other known dense granule protein of C. parvum , CpClec, is expressed in both sporozoites and merozoites and mediates the infection of C. parvum through Ca 2+ -dependent binding with sulfated proteoglycans on host intestinal epithelial cells [ 26 ]. In other apicomplexan parasites, dense granule proteins are involved in the formation of parasitophorous vacuoles and the modification of the host cell activities [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Dense Granule Metalloproteinase INS-16 in Cryptosporidium parvum

Cui

Liu

et al. 2022

IJMS

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The protozoan pathogen Cryptosporidium parvum infects intestinal epithelial cells and causes diarrhea in humans and young animals. Among the more than 20 genes encoding insulinase-like metalloproteinases (INS), two are paralogs with high sequence identity. In this study, one of them, INS-16 encoded by the cgd3_4270 gene, was expressed and characterized in a comparative study of its sibling, INS-15 encoded by the cgd3_4260 gene. A full-length INS-16 protein and its active domain I were expressed in Escherichia coli, and antibodies against the domain I and an INS-16-specific peptide were produced in rabbits. In the analysis of the crude extract of oocysts, a ~60 kDa fragment of INS-16 rather than the full protein was recognized by polyclonal antibodies against the specific peptide, indicating that INS-16 undergoes proteolytic cleavage before maturation. The expression of the ins-16 gene peaked at the invasion phase of in vitro C. parvum culture, with the documented expression of the protein in both sporozoites and merozoites. Localization studies with antibodies showed significant differences in the distribution of the native INS-15 and INS-16 proteins in sporozoites and merozoites. INS-16 was identified as a dense granule protein in sporozoites and macrogamonts but was mostly expressed at the apical end of merozoites. We screened 48 candidate INS-16 inhibitors from the molecular docking of INS-16. Among them, two inhibited the growth of C. parvum in vitro (EC50 = 1.058 µM and 2.089 µM). The results of this study suggest that INS-16 may have important roles in the development of C. parvum and could be a valid target for the development of effective treatments.

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“…Años más tarde en 1967 Ziert lo incluyó dentro del reino Protista, subreino Protozoo y phylum Sarcomastigophora. Posteriormente Silberman basado en el análisis de su pequeña subunidad de ARN (ssrNA) lo siguió incluyendo dentro de los protistas, pero como un Stramenopile (11)(12)(13) .…”

Section: Antecedentes Históricosunclassified

Análisis retrospectivo de un protozoo emergente en México: Blastocystis spp

Vega

Morales-Galicia

Tapia-Castor

et al. 2022

Mem. Inst. Investig. Cienc. Salud

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Blastocystis spp. es el protista intracelular que en los últimos años ha infectado a más de mil millones de personas a nivel mundial. Sin embargo, el aumento en la prevalencia en México y su potencial patógeno son inciertos, por lo que este microorganismo aún se encuentra bajo investigación. Principalmente a nivel pediátrico, la blastocistosis es estudiada con mayor atención, debido a que destaca sobre otros agentes en diversos estudios realizados mundialmente y en México. El objetivo de este trabajo fue mediante revisión bibliográfica, evidenciar la frecuencia y su transición parasitaria como el agente más prevalente en la actualidad, pese a que no se asocia a sintomatología clínica.

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Interaction between human mucins and parasite glycoproteins: the role of lectins and glycosidases in colonization by intestinal protozoa

Abstract: This is an open-access article distributed under the terms of the Creative Commons Attribution License.

Cited by 10 publications

References 61 publications

Dual Transcriptomics To Determine Gamma Interferon-Independent Host Response to Intestinal Cryptosporidium parvum Infection

Dual Transcriptomics To Determine Gamma Interferon-Independent Host Response to Intestinal Cryptosporidium parvum Infection

Characterization of Dense Granule Metalloproteinase INS-16 in Cryptosporidium parvum

Análisis retrospectivo de un protozoo emergente en México: Blastocystis spp

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