Interaction between cannabinoid compounds and diazepam on anxiety-like behaviour of mice

Naderi, Nima; Haghparast, Abbas; Saber-Tehrani, Ali; Rezaii, Neguine; Alizadeh, Amir-Mohammad; Khani, Abbas; Motamedi, Fereshteh

doi:10.1016/j.pbb.2007.11.001

Cited by 76 publications

(58 citation statements)

References 50 publications

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“…A recent study showed that the anxiolytic-like effect of diazepam in the EPM test could be attenuated by the CB 1 antagonist AM251, whereas a sub-effective dose of diazepam significantly augmented the effects of a sub-effective dose of URB597 (Naderi et al, 2008). Our present finding of elevated brain endocannabinoid levels following either acute or chronic treatment with diazepam might explain these intriguing previous data.…”

Section: Discussionsupporting

confidence: 75%

Anxiolytic Effects in Mice of a Dual Blocker of Fatty Acid Amide Hydrolase and Transient Receptor Potential Vanilloid Type-1 Channels

Micale

Cristino

Tamburella

et al. 2008

Neuropsychopharmacol

183

124

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The endocannabinoid-inactivating enzyme, fatty acid amide hydrolase (FAAH), and the transient receptor potential vanilloid type-1 (TRPV1) channel are new targets for the development of anxiolytic drugs. We studied the effect on anxiety-like behavior in the elevated plus maze of a dual FAAH/TRPV1 blocker, N-arachidonoyl-serotonin (AA-5-HT). In male C57BL/6J mice, acute intraperitoneal administration of AA-5-HT (0.1-2.5 mg/kg) increased both the time spent and the number of entries in the open arm, while being inactive at the highest dose tested (5 mg/kg). AA-5-HT was more potent than selective blockers of FAAH or TRPV1 (URB597 and SB366791, respectively). In male Swiss mice, AA-5-HT had to be administered chronically to observe an anxiolytic effect at an intermediate dose (2.5 mg/kg), the highest dose (5 mg/kg) being anxiogenic, and 1 mg/kg being ineffective. In both strains, the anxiolytic effects of AA-5-HT were paralleled by elevation of brain endocannabinoid levels and were reversed by per se inactive doses of the cannabinoid receptors of type-1 (CB 1 ) receptor antagonist AM251, or the TRPV1 agonist, olvanil. Immunohistochemical localization of CB 1 and TRPV1 receptors was observed in mouse prefrontal cortex, nucleus accumbens, amygdala, and hippocampus. Simultaneous 'indirect' activation of CB 1 receptors following FAAH inhibition, and antagonism at TRPV1 receptors might represent a new therapeutic strategy against anxiety.

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Section: Discussionsupporting

confidence: 75%

Anxiolytic Effects in Mice of a Dual Blocker of Fatty Acid Amide Hydrolase and Transient Receptor Potential Vanilloid Type-1 Channels

Micale

Cristino

Tamburella

et al. 2008

Neuropsychopharmacol

183

124

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“…Differences in the number of exposures to the aggressive hamsters, the dose and timing of pharmacological manipulations as well as the dependent measure assessed may account for differences between the studies. More work is necessary to determine whether URB597 could enhance effects of diazepam (Naderi et al 2008) in either the conditioned defeat model or the elevated plus maze.…”

Section: Differential Effects Of Urb597 and Diazepam In The Elevated mentioning

confidence: 99%

An endocannabinoid signaling system modulates anxiety-like behavior in male Syrian hamsters

Moise¹,

Eisenstein²,

Astarita

et al. 2008

Psychopharmacology

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Rationale-An endocannabinoid signaling system has not been identified in hamsters.Objective-We examined the existence of an endocannabinioid signaling system in Syrian hamsters using neuroanatomical, biochemical and behavioral pharmacological approaches.Method-The distribution of cannabinoid receptors was mapped and membrane fatty-acid amide hydrolase (FAAH) activity and levels of fatty-acid amides were measured in hamster brain. The impact of cannabinoid CB 1 receptor blockade and inhibition of FAAH was evaluated in the elevated plus maze, rota-rod test and models of unconditioned and conditioned social defeat.Results-A characteristic heterogeneous distribution of cannabinoid receptors was detected in hamster brain using [ 3 H]CP55,940 binding and autoradiography. The FAAH inhibitor URB597 inhibited FAAH activity (IC 50 = 12.8 nM) and elevated levels of fatty-acid amides (N-palmitoyl ethanolamine (PEA) and N-oleoyl ethanolamine (OEA)) in hamster brain. Anandamide levels were not reliably altered. The cannabinoid agonist WIN55,212-2 (1-10 mg/kg i.p.) induced CB 1 -mediated motor ataxia. Blockade of CB 1 with rimonabant (5 mg/kg i.p.) induced anxiogenic-like behavior in the elevated plus maze. URB597 (0.1-0.3 mg/kg i.p.) induced CB 1 -mediated anxiolytic-like effects in elevated plus maze, similar to the benzodiazepine diazepam (2 mg/kg i.p.). Diazepam (2-6 mg/ kg i.p.) suppressed the expression, but not the acquisition, of conditioned defeat. By contrast, neither URB597 (0.3-3.0 mg/kg i.p.) nor rimonabant (5 mg/kg i.p.) altered unconditioned or conditioned social defeat or rota-rod performance.Conclusions-Endocannabinoids engage functional CB 1 receptors in hamster brain to suppress anxiety-like behavior and undergo enzymatic hydrolysis catalyzed by FAAH. Our results further suggest that neither unconditioned nor conditioned social defeat in the Syrian hamster is dependent upon cannabinoid CB 1 receptor activation.

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“…Survey data indicate that the percentage of past-month cannabis users who report past-month use of benzodiazepines (5.6%) is greater than the number of nonusers reporting past-month prescription benzodiazepine use (0.4%) (1). Recently, it has been reported that the coadministration of exogenous cannabinoid receptor agonists and diazepam produces additive or synergistic anxiolytic effects in rodents (2). However, the mechanism(s) underlying the interaction between cannabinoid and c-aminobutyric acid (GABA)-ergic systems in the control of anxiety-like behaviors remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Binding of δ9‐tetrahydrocannabinol and diazepam to human serum albumin

et al. 2011

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Cannabis is the most commonly used illicit drug worldwide. Cannabis users also appear to use other psychoactive drugs more frequently than noncannabis users. Here, Δ9‐ tetrahydrocannabinol (THC) and diazepam binding to human serum albumin (HSA) and HSA‐heme is reported. THC binds to two different binding sites of HSA (Kd1 ≤ 10−7 M and Kd2= 10−3 M) without affecting diazepam binding (Kd = 1.2 × 10−5 M). THC binding to the high‐affinity site accounts for the low free fraction of the drug in plasma. Moreover, THC increases the affinity of heme for HSA. Accordingly, the affinity of THC for HSA‐heme is higher than that for HSA. THC could bind to FA2 and FA7 sites, as substantiated by docking simulations; nevertheless, the observed allosteric effect(s) suggests that the primary binding site of THC is the FA2 cleft that positively modulates heme affinity. Possibly, the HSA conformational transition(s) induced by THC binding could account for drug delivery to the liver through receptor‐ mediated endocytosis. © 2011 IUBMB IUBMB Life, 63(6): 446–451, 2011

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Interaction between cannabinoid compounds and diazepam on anxiety-like behaviour of mice

Cited by 76 publications

References 50 publications

Anxiolytic Effects in Mice of a Dual Blocker of Fatty Acid Amide Hydrolase and Transient Receptor Potential Vanilloid Type-1 Channels

Anxiolytic Effects in Mice of a Dual Blocker of Fatty Acid Amide Hydrolase and Transient Receptor Potential Vanilloid Type-1 Channels

An endocannabinoid signaling system modulates anxiety-like behavior in male Syrian hamsters

Binding of δ9‐tetrahydrocannabinol and diazepam to human serum albumin

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