Inter-laboratory comparison of nanoparticle size measurements using dynamic light scattering and differential centrifugal sedimentation

Langévin, D.; Lozano, Omar; Salvati, Anna; Kestens, Vikram; Monopoli, Marco P.; Raspaud, Éric; Mariot, Sandrine; Salonen, Anniina; Thomas, Steffi S.; Drießen, Marc D.; Haase, Andrea; Nelissen, Inge; Smisdom, Nick; Pompa, Pier Paolo; Maiorano, Gabriele; Puntes, Víctor; Puchowicz, Dorota; Stępnik, Maciej; Suárez, Guillaume; Riediker, Michael; Benetti, Federico; Mičetić, Ivan; Venturini, M.; Kreyling, Wolfgang G.; Zande, Meike van der; Bouwmeester, Hans; Milani, Silvia; Rädler, Joachim O.; Mülhopt, S.; Lynch, Iseult; Dawson, Kenneth A.

doi:10.1016/j.impact.2017.12.004

Cited by 61 publications

(54 citation statements)

References 46 publications

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“…The standardization of characterization methods to monitor the physicochemical properties before and after NPs' dispersion is also work in progress, e.g., by the OECD Series on the Safety of Manufactured Nanomaterials [58] and ISO/TC229 (Nanotechnologies), and has been subject of several ILC studies. Remarkably, in ILC studies in which model nanoparticles as polystyrene, gold or silica nanospheres have been used, for example those looking at size distribution measurements using nanoparticle tracking analysis [61], dynamic light scattering and centrifugal particle sedimentation [49], similar issues in reproducibility compared to our study have been reported, even for simple dispersions in buffer. They concluded that SOPs are indispensable for obtaining reliable and comparable NP size data, and should be tailored to the specific test system, sufficiently detailed and verified by a larger number of laboratories, to enable reproducible performance [49,61].…”

Section: Discussionsupporting

confidence: 72%

“…However, for NMs special care has to be taken, because of possible batch-to-batch variation, contamination and long-term stability [46]. In our study, we have selected 50-nm amine-modified polystyrene NPs (PS-NH2) and 40-nm carboxyl-modified polystyrene NPs (PS-COOH), which are known to form stable dispersions when diluted in cell culture medium [47][48][49]. Furthermore, their impact on cells has been characterized in detail [22,26,48,[50][51][52].…”

Section: Choice Of Cytotoxicity Test and Materials Usedmentioning

confidence: 99%

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Improving Quality in Nanoparticle-Induced Cytotoxicity Testing by a Tiered Inter-Laboratory Comparison Study

et al. 2020

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The quality and relevance of nanosafety studies constitute major challenges to ensure their key role as a supporting tool in sustainable innovation, and subsequent competitive economic advantage. However, the number of apparently contradictory and inconclusive research results has increased in the past few years, indicating the need to introduce harmonized protocols and good practices in the nanosafety research community. Therefore, we aimed to evaluate if best-practice training and inter-laboratory comparison (ILC) of performance of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay for the cytotoxicity assessment of nanomaterials among 15 European laboratories can improve quality in nanosafety testing. We used two well-described model nanoparticles, 40-nm carboxylated polystyrene (PS-COOH) and 50-nm amino-modified polystyrene (PS-NH2). We followed a tiered approach using well-developed standard operating procedures (SOPs) and sharing the same cells, serum and nanoparticles. We started with determination of the cell growth rate (tier 1), followed by a method transfer phase, in which all laboratories performed the first ILC on the MTS assay (tier 2). Based on the outcome of tier 2 and a survey of laboratory practices, specific training was organized, and the MTS assay SOP was refined. This led to largely improved intra- and inter-laboratory reproducibility in tier 3. In addition, we confirmed that PS-COOH and PS-NH2 are suitable negative and positive control nanoparticles, respectively, to evaluate impact of nanomaterials on cell viability using the MTS assay. Overall, we have demonstrated that the tiered process followed here, with the use of SOPs and representative control nanomaterials, is necessary and makes it possible to achieve good inter-laboratory reproducibility, and therefore high-quality nanotoxicological data.

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Section: Discussionsupporting

confidence: 72%

Section: Choice Of Cytotoxicity Test and Materials Usedmentioning

confidence: 99%

Improving Quality in Nanoparticle-Induced Cytotoxicity Testing by a Tiered Inter-Laboratory Comparison Study

et al. 2020

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“…For the other NMs, the individual particles are mostly aggregated, both in solution and in aerosols. A detailed discussion of DLS and DCS data from these as well from other QualityNano NMs is presented by Langevin et al [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Nanoparticle Batch-To-Batch Variability

et al. 2018

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A central challenge for the safe design of nanomaterials (NMs) is the inherent variability of NM properties, both as produced and as they interact with and evolve in, their surroundings. This has led to uncertainty in the literature regarding whether the biological and toxicological effects reported for NMs are related to specific NM properties themselves, or rather to the presence of impurities or physical effects such as agglomeration of particles. Thus, there is a strong need for systematic evaluation of the synthesis and processing parameters that lead to potential variability of different NM batches and the reproducible production of commonly utilized NMs. The work described here represents over three years of effort across 14 European laboratories to assess the reproducibility of nanoparticle properties produced by the same and modified synthesis routes for four of the OECD priority NMs (silica dioxide, zinc oxide, cerium dioxide and titanium dioxide) as well as amine-modified polystyrene NMs, which are frequently employed as positive controls for nanotoxicity studies. For 46 different batches of the selected NMs, all physicochemical descriptors as prioritized by the OECD have been fully characterized. The study represents the most complete assessment of NMs batch-to-batch variability performed to date and provides numerous important insights into the potential sources of variability of NMs and how these might be reduced.

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“…Characterization of the PSNPs, by determining the hydrodynamic size and zeta-potential of the PSNPs in cell culture medium, showed that all 50 nm PSNP suspensions were stable during the exposure times in the experiments. The DLS method as used here has been evaluated in a inter laboratory testing project [ 31 ] to characterize the size of the PSNPs in water or DMEM + and it was found that the proteins present in the cell culture medium resulted in a signal indicating a hydrodynamic diameter of 27 ± 2 nm. The zeta-potential of all PSNPs in cell culture medium was comparable and stable over time, irrespective of the PSNP surface chemistry with a negative charge on the PSNPs’ surface results from the PSNPs, the proteins adsorbed on the surface of the PSNPs or from measuring protein aggregates of medium rather than PSNPs [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport

et al. 2018

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BackgroundUpon ingestion, nanoparticles can interact with the intestinal epithelial barrier potentially resulting in systemic uptake of nanoparticles. Nanoparticle properties have been described to influence the protein corona formation and subsequent cellular adhesion, uptake and transport. Here, we aimed to study the effects of nanoparticle size and surface chemistry on the protein corona formation and subsequent cellular adhesion, uptake and transport. Caco-2 intestinal cells, were exposed to negatively charged polystyrene nanoparticles (PSNPs) (50 and 200 nm), functionalized with sulfone or carboxyl groups, at nine nominal concentrations (15–250 μg/ml) for 10 up to 120 min. The protein coronas were analysed by LC–MS/MS.ResultsSubtle differences in the protein composition of the two PSNPs with different surface chemistry were noted. High-content imaging analysis demonstrated that sulfone PSNPs were associated with the cells to a significantly higher extent than the other PSNPs. The apparent cellular adhesion and uptake of 200 nm PSNPs was not significantly increased compared to 50 nm PSNPs with the same surface charge and chemistry. Surface chemistry outweighs the impact of size on the observed PSNP cellular associations. Also transport of the sulfone PSNPs through the monolayer of cells was significantly higher than that of carboxyl PSNPs.ConclusionsThe results suggest that the composition of the protein corona and the PSNP surface chemistry influences cellular adhesion, uptake and monolayer transport, which might be predictive of the intestinal transport potency of NPs.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0394-6) contains supplementary material, which is available to authorized users.

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Inter-laboratory comparison of nanoparticle size measurements using dynamic light scattering and differential centrifugal sedimentation

Abstract: Inter-laboratory comparison of nanoparticle size measurements using dynamic light scattering and differential centrifugal sedimentation. NanoImpact, (2018).

Cited by 61 publications

References 46 publications

Improving Quality in Nanoparticle-Induced Cytotoxicity Testing by a Tiered Inter-Laboratory Comparison Study

Improving Quality in Nanoparticle-Induced Cytotoxicity Testing by a Tiered Inter-Laboratory Comparison Study

Characterization of Nanoparticle Batch-To-Batch Variability

Impact of nanoparticle surface functionalization on the protein corona and cellular adhesion, uptake and transport

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