Integrins αvβ3 and αvβ5 promote adenovirus internalization but not virus attachment

Wickham, Thomas J.; Mathias, Patricia; Cheresh, David A.; Nemerow, Glen R.

doi:10.1016/0092-8674(93)90231-e

Cited by 2,046 publications

(1,668 citation statements)

References 39 publications

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“…In addition, the Kriegstein group used adenoviral labeling (Hansen et al, 2010; LaMonica et al, 2013; Ostrem et al, 2014) to label OSVZ precursors. Adenoviruses infect noncycling and cycling cells but are preferentially internalized in precursors bearing integrins (Wickham et al, 1993). Integrins are concentrated at the distal tip of the basal process of the bRG that extends a process up to the basal membrane (Fietz et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Unsupervised lineage‐based characterization of primate precursors reveals high proliferative and morphological diversity in the OSVZ

Pfeiffer

Betizeau

Waltispurger

et al. 2015

J of Comparative Neurology

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Generation of the primate cortex is characterized by the diversity of cortical precursors and the complexity of their lineage relationships. Recent studies have reported miscellaneous precursor types based on observer classification of cell biology features including morphology, stemness, and proliferative behavior. Here we use an unsupervised machine learning method for Hidden Markov Trees (HMTs), which can be applied to large datasets to classify precursors on the basis of morphology, cell‐cycle length, and behavior during mitosis. The unbiased lineage analysis automatically identifies cell types by applying a lineage‐based clustering and model‐learning algorithm to a macaque corticogenesis dataset. The algorithmic results validate previously reported observer classification of precursor types and show numerous advantages: It predicts a higher diversity of progenitors and numerous potential transitions between precursor types. The HMT model can be initialized to learn a user‐defined number of distinct classes of precursors. This makes it possible to 1) reveal as yet undetected precursor types in view of exploring the significant features of precursors with respect to specific cellular processes; and 2) explore specific lineage features. For example, most precursors in the experimental dataset exhibit bidirectional transitions. Constraining the directionality in the HMT model leads to a reduction in precursor diversity following multiple divisions, thereby suggesting that one impact of bidirectionality in corticogenesis is to maintain precursor diversity. In this way we show that unsupervised lineage analysis provides a valuable methodology for investigating fundamental features of corticogenesis. J. Comp. Neurol. 524:535–563, 2016. © 2015 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Unsupervised lineage‐based characterization of primate precursors reveals high proliferative and morphological diversity in the OSVZ

Pfeiffer

Betizeau

Waltispurger

et al. 2015

J of Comparative Neurology

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“…[9][10][11][12] Second, viral penton base binds to ␣v␤3 or ␣v␤5 integrins. 13,14 The interaction of penton base with cell surface integrins is thought to have several effects. It may facilitate infection by pH-dependent membrane permeabilization and release of the virus from the endosome into the cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

“…17,18 The importance of the interaction between adenovirus penton base and cell surface ␣v integrin has been shown during infection of M21, CS-1, A549, HeLa and THP-1 cell lines. 13,14,16,19,20 For example, M21 cells expressing ␣v integrins were more readily infected by adenovirus than were ␣v integrin-negative M21 cells. 13 ␣v Integrins recognize the RGD sequence in adenovirus penton base.…”

Section: Introductionmentioning

confidence: 99%

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An interaction between penton base and αv integrins plays a minimal role in adenovirus-mediated gene transfer to hepatocytes in vitro and in vivo

et al. 1998

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Studies in cultured cell lines have shown that adenovirus tion of hepatocytes, even though the mutation impaired infection involves binding of adenovirus fiber to its cell surinfection of HeLa cells. Hepatocytes had undetectable face receptor and binding of penton base to ␣v integrins.amounts of ␣v integrins on their cell surface and showed However, much less is known about the role of these interno specific adherence to vitronectin, the natural substrate actions in cells that are targets for adenovirus-mediated of ␣v integrins. Adenovirus with an intact penton base gene transfer. Earlier work showed that hepatocytes are enhanced infection of liver following intravenous injection, readily infected by adenovirus, making them an attractive but only by three-fold as compared with virus in which the target for gene therapy in several diseases. We found that integrin-binding motif was disrupted. These studies sugaddition of fiber protein blocked adenovirus infection of prigest that interactions between cell surface integrins and mary cultures of hepatocytes. This suggests an important penton base are not required for adenovirus infection of role for fiber and its receptor. However, mutation of the hepatocytes in vitro, but the interaction enhances infection integrin-binding motif in penton base did not inhibit infecto a small degree in vivo.

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“…Studies on adenovirus type 2 have shown that the cell surface integrin ␣ v ␤ 3 /␣ v ␤ 5 subunits are required for virus internalization. 23,24 Recently, Acsadi et al 25 showed that cultured myoblasts expressed increased levels of ␤ 3 /␤ 5 integrins which directly correlated with their susceptibility to adenovirus infection.Based on the observation that B-CLL cells, when activated with IL-4 plus anti-CD40 mAb/CD40L, show surface before or after the activation. …”

mentioning

confidence: 99%

Efficient adenovirus-mediated gene transduction of normal and leukemic hematopoietic cells

et al. 1997

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We evaluated the efficiency of adenovirus-mediated gene targeted cells. A novel protocol for the efficient transduction transfer into normal and malignant human hematopoietic of adenovirus into B-CLL cells was presented. We showed cells. An E-1 and E-3 deleted, replication-defective recomthat anti-CD40 mAb or CD40 ligand acts in synergy with binant Ad.RSV␤gal vector was used and the transduction rhIL-4 to enable the transduction of approximately 50-75% efficiency was studied at a multiplicity of infection of 13 of B-CLL cells (B-CLL, n = 6). Expression of ␤-galactop.f.u. per cell. Approximately 40-50% of normal monocytes sidase in transduced CML cells and B-CLL cells was were transduced, whereas purified normal resting T cells detected for at least 15 days after transduction. The and B cells were resistant to infection. We showed that 50-present studies underline the utility of adenovirus vectors 80% of primary chronic myeloid leukemia cells (CML, n = for the construction of cytokine gene-modified tumor vac-12) were efficiently transduced. In contrast to CML, succines for the treatment of hematopoietic malignancies such cessful transduction of resting primary chronic B lymphoas CML and B-CLL. cytic leukemia cells required appropriate preactivation of for 3 days with rhGM-CSF (100 U/ml) before infection. Activation of cells with GM-CSF did not influence the (CD14 + , 90-98%) were readily transduced and stained transduction rate (data not shown). strongly positive for ␤-galactosidase (five separate experiments). Unlike the experience with retrovirus, we rhIL-4 together with hCD40 ligand are efficient and showed that efficient transduction of normal peripheral necessary for the transduction of primary B-CLL cells blood monocytes can be achieved with adenovirus withMononuclear cells from chronic B lymphocytic leukemia out preactivation of target cells. In contrast, purified (B-CLL, n = 6) patients were studied for adenoviral vector resting T lymphocytes (CD3 + , Ͼ96%) and B lymphocytes transduction. B-CLL cells were Ig-light chain restricted (CD19 + , Ͼ95%) were, however, not permissive to transand displayed CD5 (100%) and CD19 (80-95%) on the duction (three separate experiments).cell surface. Similarly to normal resting B lymphocytes (Figure 1), Efficient transduction of primary CML cells without resting primary B-CLL cells were resistant to adenovirus preactivation of target cells infection. A number of activation protocols [15][16][17][18] were Unselected mononuclear cells from chronic myeloid leutested to improve the transduction efficacy of B-CLL kemia patients (CML, n = 12) were included in the cells. We found that neither phorbol ester induction nor present studies. Although all the CML cells were Ph + , but the combination of SAC + thioredoxin + IL-2, forskolin represented heterogeneous cell populations. CML cells nor TNF␣ were efficient in improving the transduction expressed CD13 (40-80%), CD33 (40-81%) and other cell rate. In marked contrast, approximately 70-75% B-CLL surface markers (Table 1). No sign...

show abstract

Integrins αvβ3 and αvβ5 promote adenovirus internalization but not virus attachment

Cited by 2,046 publications

References 39 publications

Unsupervised lineage‐based characterization of primate precursors reveals high proliferative and morphological diversity in the OSVZ

Unsupervised lineage‐based characterization of primate precursors reveals high proliferative and morphological diversity in the OSVZ

An interaction between penton base and αv integrins plays a minimal role in adenovirus-mediated gene transfer to hepatocytes in vitro and in vivo

Efficient adenovirus-mediated gene transduction of normal and leukemic hematopoietic cells

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