Integrative proteome analysis of Brachypodium distachyon roots and leaves reveals a synergetic responsive network under H 2 O 2 stress

Bian, Yanwei; Lv, Dongwen; Cheng, Zhiwei; Gu, Aiqin; Cao, Hui; Yan, Yueming

doi:10.1016/j.jprot.2015.08.020

Cited by 26 publications

(32 citation statements)

References 69 publications

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“…In particular, KOG0841 (14-3-3-like protein A) was involved in interactions with proteins from other functional groups in both roots and leaves, suggesting it has an important signaling role in both organs. Similar results were obtained from Bd21 seedlings subjected to H 2 O 2 stress26.…”

Section: Resultssupporting

confidence: 86%

“…7). Our recent work also demonstrated that 14-3-3-like protein A transcripts and protein accumulated in response to salt stress in B. distachyon leaves33, while other 14-3-3 family members including 14-3-3-like proteins GF14-B and GF14-D also showed differential accumulation under H 2 O 2 stress in both roots and leaves26. The PPI network generated in this study demonstrates that 14-3-3 proteins interact with many other proteins that have diverse functions, suggesting 14-3-3 proteins have important roles in defense against drought stress.…”

Section: Discussionmentioning

confidence: 56%

“…Increased ROS production might enhance ABA accumulation, while higher levels of ABA could generate more ROS production in guard cells, creating a positive feedback loop and mediating stomatal closure24. In addition, drought also leads to changes in plant calcium (Ca 2+ ) concentrations that can trigger signaling cascades25, and high concentrations of ROS can produce synergistic responses in both plant roots and leaves26.…”

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confidence: 99%

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Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Bian

Deng

Xing

et al. 2017

Sci Rep

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In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.Plants encounter a variety of biotic and abiotic stresses during growth 1 . These stresses unbalance cellular homeostasis and lead to morphological, physiological, and molecular changes 2 . These changes have a negative impact on survival and biomass production, and can reduce final yield by up to 82% 3 . Global warming and climate change may also be exacerbating the effects of abiotic stresses on crop production in many areas of the world today. Previous reports have suggested that a temperature increase of 1 °C can produce a decrease in yield of up to 10% 4 . Drought in particular, severely impairs plant growth and development and limits crop productivity more than any other environmental factor 5,6 . As the climate continues to change, drought may become a more frequent and cause severe problem 7 . Drought stress induces a range of physiological and biochemical responses in plants. Under drought conditions, the plant root cap produces abscisic acid (ABA), which mediates stomatal closure. This in turn, suppresses cell growth, photosynthetic efficiency, and respiration [8][9][10] . Recently, ABA and stomata have been implicated in the regulation of systemic responses to abiotic stresses 11 . In addition, plants generate toxic ions and reactive oxygen species (ROS) that can decrease enzyme activities and damage essential proteins 12 . The generation of ROS occurs early in the response to water-stress, and ROS are key secondary messengers triggering subsequent defensive measures in plants 1,[13][14][15] . Plants have developed a sophisticated and elaborate system for scavenging high levels of ROS using antioxidant enzymes that include superoxid...

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Section: Resultssupporting

confidence: 86%

Section: Discussionmentioning

confidence: 56%

mentioning

confidence: 99%

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Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Bian

Deng

Xing

et al. 2017

Sci Rep

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“…Proteome analysis of roots and leaves revealed a synergetic responsive network under stress [12]. The DIGE analysis results showed that 55 protein spots were significantly changed as a result of salinity and drought stress.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, proteome of plant species changes as a response to biotic “pests” [7–9] and abiotic “chemical, salt, and drought stress” [3, 10, 11]. Proteome analysis of roots and leaves revealed a synergetic responsive network under stress; roots rapidly sensed and responded to stress, after which the stress signals were transferred to leaves and both roots and leaves showed similar metabolic pathways under stress with distinct changes [12]. Under salinity, salt is transported via the xylem to the shoot causing the accumulation of Na + and Cl − ions in shoot cells to a toxic extent, resulting in ionic stress that leads to ion accumulation enhancing the production of reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Rabey

Al-Malki

Abulnaja

2016

International Journal of Genomics

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Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition.

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Low‐molecular‐weight glutenin subunits from the 1U genome of Aegilops umbellulata confer superior dough rheological properties and improve breadmaking quality of bread wheat

Wang

Zhen

et al. 2017

J Sci Food Agric

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Integrative proteome analysis of Brachypodium distachyon roots and leaves reveals a synergetic responsive network under H 2 O 2 stress

Cited by 26 publications

References 69 publications

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Proteome Analysis of Date Palm (Phoenix dactyliferaL.) under Severe Drought and Salt Stress

Low‐molecular‐weight glutenin subunits from the 1U genome of Aegilops umbellulata confer superior dough rheological properties and improve breadmaking quality of bread wheat

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