Integrative analysis of epigenomics, transcriptomics, and proteomics to identify key targets and pathways of Weining granule for gastric cancer

Liang, Mingkun; Liang, Xingqiu; Zhong, Jing; Wei, Yuting; Zhou, Lian; Huang, Zhengkai; Liang, Jian

doi:10.1016/j.jep.2021.113787

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…miR-20b inhibits TXNIP, which is involved in the PI3K/AKT/mTOR pathway to promote the GC progression by mediating glucose uptake in cancer cells [ 118 ]. TXNIP may be a therapeutic target of Weining granule (WNG) for GC [ 119 ].…”

Section: The Role Of Txnip In Diseasementioning

confidence: 99%

TXNIP: A Double-Edged Sword in Disease and Therapeutic Outlook

Pan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Thioredoxin-interacting protein (TXNIP) was originally named vitamin D3 upregulated protein-1 (VDUP1) because of its ability to bind to thioredoxin (TRX) and inhibit TRX function and expression. TXNIP is an alpha-arrestin protein that is essential for redox homeostasis in the human body. TXNIP may act as a double-edged sword in the cell. The balance of TXNIP is crucial. A study has shown that TXNIP can travel between diverse intracellular locations and bind to different proteins to play different roles under oxidative stress. The primary function of TXNIP is to induce apoptosis or pyroptosis under oxidative stress. TXNIP also inhibits proliferation and migration in cancer cells, although TXNIP levels decrease, and function diminishes in various cancers. In this review, we summarized the main structure, binding proteins, pathways, and the role of TXNIP in diseases, aiming to explore the double-edged sword role of TXNIP, and expect it to be helpful for future treatment using TXNIP as a therapeutic target.

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Section: The Role Of Txnip In Diseasementioning

confidence: 99%

TXNIP: A Double-Edged Sword in Disease and Therapeutic Outlook

Pan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Proteomics can detect the full set of proteins expressed by the complete genome in a cell [ 29 , 30 ]. This technology can visually identify the regulation of drugs at the cellular protein level [ 31 ], then find targets and related pathways [ 32 ]. Therefore, on the basis of our experimental results, we confirmed for the first time that GBN can inhibit the apoptosis, migration, and invasion of SW620 and SW480 cells.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Effects of Glabridin on the Proliferation, Apoptosis, and Migration of the Human Colon Cancer Cell Lines SW480 and SW620 and Its Mechanism Based on Reverse Virtual Screening and Proteomics

Xia

et al. 2023

Oxidative Medicine and Cellular Longevity

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Colon cancer is a relatively common malignant tumor of the digestive tract. Currently, most colon cancers originate from adenoma carcinogenesis. By screening various licorice flavonoids with anticancer effects, we found that glabridin (GBN) has a prominent anticolon cancer effect. First, we initially explored whether GBN can inhibit proliferation, migration, and invasion and induce apoptosis in SW480 and SW620 cells. Next, we exploited reverse virtual and proteomics technologies to screen out closely related target pathways on the basis of a drug and target database. At the same time, we constructed the structure of the GBN target pathway in colon cancer. We predicted that GBN can regulate the phosphatidylinositol 3-kinase (PI3K)–protein kinase B (AKT)–mammalian target of the rapamycin pathway (mTOR) pathway to fight colon cancer. Finally, through Western blot analysis and qRT-PCR, we verified that the expression levels of the PI3K, AKT, and mTOR proteins and genes in this pathway were significantly reduced after GBN administration. In short, the promising discovery of the anticolon cancer mechanism of GBN provides a reliable experimental basis for subsequent new drug development.

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“…The application of proteomics assays has offered preliminary biological evidence for the potential utility of Chinese herbal medicines in treating gastric cancer. For example, Tan et al (2019) (2021)showed that WNG treatment altered the abundance of 192 proteins in gastric cancer cells. These proteins were associated with carbon metabolism, cholesterol metabolism, alanine, glutamate and aspartate metabolism, and TNF signaling pathways in gastric cancer.…”

Section: Proteomicsmentioning

confidence: 99%

“…Based on 1H-NMR spectroscopy, Gnocchi et al (2021)found that the ethyl acetate extract of Crithmum maritimum can reverse the Warburg effect (preferential use of glycolysis rather than oxidative phosphorylation for energy production by tumor cells) in HCC cells by decreasing intracellular lactate. The ethyl acetate extract can also inhibit protein anabolism by reducing the intracellular level of amino acids and alter membrane biosynthesis by lowering choline and phosphocholine contents in HCC.…”

Section: Metabolomicsmentioning

confidence: 99%

Using omics approaches to dissect the therapeutic effects of Chinese herbal medicines on gastrointestinal cancers

Wang

et al. 2022

Front. Pharmacol.

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Chinese herbal medicines offer a rich source of anti-cancer drugs. Differences between the pharmacology of Chinese herbal medicines and modern synthetic chemicals hinder the development of drugs derived from herbal products. To address this challenge, novel omics approaches including transcriptomics, proteomics, genomics, metabolomics, and microbiomics have been applied to dissect the pharmacological benefits of Chinese herbal medicines in cancer treatments. Numerous Chinese herbal medicines have shown potential anti-tumor effects on different gastrointestinal (GI) cancers while eliminating the side effects associated with conventional cancer therapies. The present study aimed to provide an overview of recent research focusing on Chinese herbal medicines in GI cancer treatment, based on omics approaches. This review also illustrates the potential utility of omics approaches in herbal-derived drug discovery. Omics approaches can precisely and efficiently reveal the key molecular targets and intracellular interaction networks of Chinese herbal medicines in GI cancer treatment. This study summarizes the application of different omics-based approaches in investigating the effects and mechanisms of Chinese herbal medicines in GI cancers. Future research directions are also proposed for this area of study.

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Integrative analysis of epigenomics, transcriptomics, and proteomics to identify key targets and pathways of Weining granule for gastric cancer

Cited by 8 publications

References 37 publications

TXNIP: A Double-Edged Sword in Disease and Therapeutic Outlook

TXNIP: A Double-Edged Sword in Disease and Therapeutic Outlook

Investigation of the Effects of Glabridin on the Proliferation, Apoptosis, and Migration of the Human Colon Cancer Cell Lines SW480 and SW620 and Its Mechanism Based on Reverse Virtual Screening and Proteomics

Using omics approaches to dissect the therapeutic effects of Chinese herbal medicines on gastrointestinal cancers

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