Integration of molecular and enzymatic catalysts on graphene for biomimetic generation of antithrombotic species

Abstract

The integration of multiple synergistic catalytic systems can enable the creation of biocompatible enzymatic mimics for cascading reactions under physiologically relevant conditions. Here we report the design of a graphene–haemin–glucose oxidase conjugate as a tandem catalyst, in which graphene functions as a unique support to integrate molecular catalyst haemin and enzymatic catalyst glucose oxidase for biomimetic generation of antithrombotic species. Monomeric haemin can be conjugated with graphene through π–π interactions to function as an effective catalyst for the oxidation of endogenous L-arginine by hydrogen peroxide. Furthermore, glucose oxidase can be covalently linked onto graphene for local generation of hydrogen peroxide through the oxidation of blood glucose. Thus, the integrated graphene–haemin–glucose oxidase catalysts can readily enable the continuous generation of nitroxyl, an antithrombotic species, from physiologically abundant glucose and L-arginine. Finally, we demonstrate that the conjugates can be embedded within polyurethane to create a long-lasting antithrombotic coating for blood-contacting biomedical devices.

Solar‐Energy‐Driven Photoelectrochemical Biosensing Using TiO2 Nanowires

“…[1][2][3][4][5][6][7][8][9][10][11][12][13] With the ultimate goal of achieving fast, sensitive, large-scale, and low-cost molecular analysis, aw ide variety of detection technologies have been developed, such as fluorescent, [14] spectroscopic, [15] electrical, [16] magnetic, [17] and mechanical methods. [1][2][3][4][5][6][7][8][9][10][11][12][13] With the ultimate goal of achieving fast, sensitive, large-scale, and low-cost molecular analysis, aw ide variety of detection technologies have been developed, such as fluorescent, [14] spectroscopic, [15] electrical, [16] magnetic, [17] and mechanical methods.…”

The Potential Role of Graphene in Developing the Next Generation of Endomaterials

“…Additionally, the reactive groups of GO facilitate chemical interaction and conjugation with an extensive variety of molecules [40, 78–82]. There are already limited data that graphene and especially GO have a great potential in becoming such a drug-eluting delivery system that can reduce endothelial proliferation and restenosis in grafts [22, 26, 27, 33, 83]. A study on the release of anti-inflammatory medication by carbon coating is of relevance to abdominal aortic aneurysm (AAA) repair and the postimplantation syndrome [84].…”

“…Weaver et al developed a GO-based delivery system controlled by electrical impulses to release dexamethasone into the bloodstream [30]. Other studies have reported the transport and release of antithrombotic agents by graphene [31, 32]. …”

“…Xue et al used graphene coating in vitro as a substrate for integrating antithrombotic enzymic systems [33]. …”

3D graphene/nylon rope as a skeleton for noble metal nanocatalysts for highly efficient heterogeneous continuous-flow reactions

“…[24][25][26][27][28] Noble metal graphene composites have been widely explored for chemical reactions, with improved catalytic activity and enhanced durability. [24][25][26][27][28] Noble metal graphene composites have been widely explored for chemical reactions, with improved catalytic activity and enhanced durability.…”