Integrated Proteomics and Metabolomics Link Acne to the Action Mechanisms of Cryptotanshinone Intervention

Abstract

The label-free methods of proteomic combined with metabolomics were applied to explore the mechanisms of Cryptotanshinone (CPT) intervention in rats with acne. The model group consisted of rats given oleic acid (MC), then treated with CPT, while control groups did not receive treatment. The skin samples were significantly different between control, model and CPT-treated groups in hierarchical clustering dendrogram. Obvious separations of the skin metabolic profiles from the three groups were found through PCA scoring. In total, 231 and 189 differentially expressed proteins (DEPs) were identified in MC and CPT groups, respectively. By the KEGG analysis, five protein and metabolite pathways were found to be significantly altered. These played important roles in response to oleic acid-induced acne and drug treatment. CPT could negatively regulate glycolysis/gluconeogenesis and histidine metabolisms to decrease keratinocyte differentiation and improve excessive keratinization and cellular barrier function. CPT could down-regulate the IL-17 signaling pathway and regulate the acne-driven immune response of sebum cells. The biosynthesis of unsaturated fatty acids metabolism, glycerophospholipid metabolism and linoleic acid pathways could significantly alter sebum production and control sebaceous gland secretion after CPT treatment. The gap junction was up-regulated after CPT treatment and the skin barrier turned back to normal. Krt 14, Krt 16 and Krt 17 were significantly down-regulated, decreasing keratinization, while inflammatory cell infiltration was improved by down-regulation of Msn, up-regulation of linoleic acid and estrogen pathways after CPT treatment. These results propose action mechanisms for the use of CPT in acne, as a safe and potential new drug.

Current Knowledge in Skin Metabolomics: Updates from Literature Review

“…Several other dermatological conditions have recently been studied from the metabolomic point of view, including inflammatory infectious and neoplastic conditions [ 80 , 89 , 104 , 106 ]. Increased levels of L-glutamate have been found in skin samples of cutaneous T cell lymphomas and adjacent skin, together with decreased adenosine monophosphate [ 65 ].…”

Comparative Proteomics Analysis Reveals the Reversal Effect of Cryptotanshinone on Gefitinib-Resistant Cells in Epidermal Growth Factor Receptor-Mutant Lung Cancer

“…Concerning the relationship between SCD and AMPK, a study showed that inhibition of SCD1 in cancer cells promoted the activation of AMPK and the subsequent reduction of glucose-mediated lipogenesis ( Scaglia et al, 2009 ). In addition, an integrated proteomic and metabolomic research on CTS in treating acne showed that CTS remedies abnormalities in unsaturated fatty acid synthetic enzymes and metabolites ( Zhu et al, 2021 ). Therefore, it is possible that CTS modulates lipid and fatty acid metabolism in resistant cancer cells by regulating the SCD and AMPK pathways.…”

Studi Jamur Aspergillus fumigatus di Pasar Cakranegara Kota Mataram Penyebab Penyakit Aspergillosis Menggunakan Media Pertumbuhan Potato Dextrose Agar

“…Aspergillosis merupakan suatu kelompok mikosis yang disebabkan oleh infeksi jamur spesies Aspergillus yaitu Aspergillus fumigatus (Bertuzzi et al, 2021;Zhu et al, 2021). Jamur Aspergillus fumigatus merupakan penyebab infeksi pada manusia yang terbanyak, dimana > 90% menyebabkan invasif dan noninvasif aspergillosis.…”