Integrated network pharmacology and cellular assay for the investigation of an anti-obesity effect of 6-shogaol

Jiao, Wenya; Mi, Si; Sang, Yaxin; Jin, Qiuxia; Chitrakar, Bimal; Wang, Xianghong; Wang, Shuo

doi:10.1016/j.foodchem.2021.131755

Cited by 42 publications

(25 citation statements)

References 34 publications

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“…Screening out the effective components from traditional Chinese medicine through network pharmacology can provide a basis for more targeted research on active components from traditional Chinese medicine. 25 Our pathway enrichment studies showed that the main targets of the four compounds in the leaves of Isodon suzhouensis were concentrated in the TNF signaling pathway and the cancer signaling pathways. Therefore, when the body is under oxidative stress, Isodon suzhouensis may relieve symptoms through regulating these pathways.…”

Section: Discussionmentioning

confidence: 86%

“…Network pharmacology can reflect the complex interaction between biological macromolecules and chemical components to a large extent. Screening out the effective components from traditional Chinese medicine through network pharmacology can provide a basis for more targeted research on active components from traditional Chinese medicine . Our pathway enrichment studies showed that the main targets of the four compounds in the leaves of Isodon suzhouensis were concentrated in the TNF signaling pathway and the cancer signaling pathways.…”

Section: Discussionmentioning

confidence: 89%

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Determination of the Anti-Oxidative Stress Mechanism of Isodon suzhouensis Leaves by Employing Bioinformatic and Novel Research Technology

Wang

Lv³

et al. 2023

ACS Omega

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Isodon suzhouensis from Suzhou, China, is a traditional Chinese herb with wide applications in medicine and food. The antioxidant activity against oxidative stress of the leaves of Isodon suzhouensis is a myth since long and is not explored earlier thoroughly. The present study is focused to explore the active components in Isodon suzhouensis leaf extracts responsible for antioxidant effects against oxidative stress and the potential mechanism of this activity. We obtained the chromatograms of Isodon suzhouensis leaf extracts by the high-performance liquid phase (HPLC) for possible detection of antioxidant constituents. Some compounds in Isodon suzhouensis leaf extracts were then further assessed through the luminol luminescence mechanism combined with HPLC analysis as well as with SwissTargetPrediction database that helped to screen out the other constituents. The targets for effects against oxidative stress were then further screened through the GeneCards database, and the PPI network was constructed. The targets were analyzed by GO and KEGG using the David database. The obtained results were then further studied by employing in vitro experimentation and protein expression analyses by Western blotting. It is found that Isodon suzhouensis leaf extracts contain rutin, isoquercetin, glaucocalyxin A, glaucocalyxin B, and other compounds with antioxidant activity. The activity map of the free radical scavenging signals from Isodon suzhouensis showed a strong ability to scavenge free radicals with the highest capacity of glaucocalyxin B followed by isoquercetin succeeding the glaucocalyxin A supervening the rutin. Further network pharmacological analyses and in vitro experimentation showed that Isodon suzhouensis leaf extracts interfere with TNF and the p38 MAPK signaling pathway for antioxidant effects against oxidative stress. Conclusively, it is found that Isodon suzhouensis leaf extracts possess strong antioxidant potential via targeting TNF and p38 MAPK signaling pathways against oxidative stress, providing scientific foundation for further studies on Isodon suzhouensis for the further therapeutic approach.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 89%

Determination of the Anti-Oxidative Stress Mechanism of Isodon suzhouensis Leaves by Employing Bioinformatic and Novel Research Technology

Wang

Lv³

et al. 2023

ACS Omega

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“…Utilizing the STRING database (https://cn.string-db.org/), a protein‒protein interaction (PPI) network was built that intersected the targets; then, the “tsv” file was downloaded and imported into Cytoscape software for visual analysis. Twice the median of the degree value was used as the topological index to screen core targets (Jiao et al., 2022).…”

Section: Methodsmentioning

confidence: 99%

Effect of ferulic acid and p‐coumaric acid on lowering uric acid through network pharmacology and in vitro studies

Li,

Liu,

Jiao

et al. 2024

Food Safety and Health

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The occurrence of hyperuricemia is increasing yearly. Based on network pharmacology, this study predicted the molecular targets and signaling pathways of ferulic acid and p‐coumaric acid for improving hyperuricemia. The results were verified through in vitro cell experiments. After enrichment analysis of 11 core targets of ferulic acid and p‐coumaric acid to improve hyperuricemia, the phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) signaling pathway was considered as the most significant signaling pathway. In the hyperuricemia cell model, ferulic acid and p‐coumaric acid significantly increased cell viability and decreased the cell uric acid (UA) content. Ferulic acid and p‐coumaric acid significantly regulated the expression of UA transport‐related proteins, namely urate organic anion transporter 1, glucose transporter 9, and adenosine triphosphate‐binding transporter protein G2. Ferulic acid and p‐coumaric acid also downregulated the phosphorylation of PI3K and Akt, which inhibited the PI3K/Akt signaling pathway. This study confirmed that ferulic acid and p‐coumaric acid could regulate UA‐related proteins through the PI3K/Akt pathway and promote UA excretion to alleviate hyperuricemia. The results of this research provided a theoretical basis for further research and development of UA‐lowering products.

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“…Network pharmacology is a promising way to reveal the relationship between small molecules, therapeutic targets, and diseases. With the aid of a biological network, it is convenient to explore the potential targets and mechanisms of pharmacological ingredients 22 . Based on previous studies, we assumed that PA can protect cognitive function by modulating inflammation in AD.…”

Section: Introductionmentioning

confidence: 99%

Polygalacic acid attenuates cognitive impairment by regulating inflammation through PPARγ/NF‐κB signaling pathway

Zhao,

Jia

2024

CNS Neurosci Ther

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AimsWe aimed to explore the role and molecular mechanism of polygalacic acid (PA) extracted from traditional Chinese medicine Polygala tenuifolia in the treatment of Alzheimer's disease (AD).MethodsThe network pharmacology analysis was used to predict the potential targets and pathways of PA. Molecular docking was applied to analyze the combination between PA and core targets. Aβ42 oligomer‐induced AD mice model and microglia were used to detect the effect of PA on the release of pro‐inflammatory mediators and its further mechanism. In addition, a co‐culture system of microglia and neuronal cells was constructed to assess the effect of PA on activating microglia‐mediated neuronal apoptosis.ResultsWe predict that PA might regulate inflammation by targeting PPARγ‐mediated pathways by using network pharmacology. In vivo study, PA could attenuate cognitive deficits and inhibit the expression levels of inflammation‐related factors. In vitro study, PA can also decrease the production of activated microglia‐mediated inflammatory cytokines and reduce the apoptosis of N2a neuronal cells. PPARγ inhibitor GW9662 inversed the neuroprotective effect of PA. Both in vivo and in vitro studies showed PA might attenuate the inflammation through the PPARγ/NF‐κB pathway.ConclusionsPA is expected to provide a valuable candidate for new drug development for AD in the future.

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Integrated network pharmacology and cellular assay for the investigation of an anti-obesity effect of 6-shogaol

Cited by 42 publications

References 34 publications

Determination of the Anti-Oxidative Stress Mechanism of Isodon suzhouensis Leaves by Employing Bioinformatic and Novel Research Technology

Determination of the Anti-Oxidative Stress Mechanism of Isodon suzhouensis Leaves by Employing Bioinformatic and Novel Research Technology

Effect of ferulic acid and p‐coumaric acid on lowering uric acid through network pharmacology and in vitro studies

Polygalacic acid attenuates cognitive impairment by regulating inflammation through PPARγ/NF‐κB signaling pathway

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