Integrated delignification and simultaneous saccharification and fermentation of hard wood by a white-rot fungus, Phlebia sp. MG-60

Kamei, Ichiro; Hirota, Yoshiyuki; Meguro, Sadatoshi

doi:10.1016/j.biortech.2012.09.007

Cited by 76 publications

(26 citation statements)

References 21 publications

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“…However the high activities of both enzymes were detected 120 h after the culture was changed to the semi-aerobic liquid culture conditions (Kamei et al 2012b). These results demonstrated that a clear switch of metabolism from selective lignin degradation to polysaccharide degradation occurred at the transition from aerobic-solid culture conditions to semi-aerobic-liquid culture conditions (Kamei et al 2012b). This phenomenon might also have been affected by the water content.…”

Section: Cbp Fermentation Of High-concentration Uhkpmentioning

confidence: 86%

“…Thus, a new process (Integrated Fungal Fermentation: IFF) of unified aerobic delignification and anaerobic saccharification and fermentation of wood by Phlebia sp. MG-60 was proposed (Kamei et al 2012b). This fungus is able to selectively degrade lignin under aerobic solid state fermentation conditions and to produce ethanol directly from delignified oak wood under semi-aerobic liquid culture conditions.…”

Section: Introductionmentioning

confidence: 99%

“…This fungus is able to selectively degrade lignin under aerobic solid state fermentation conditions and to produce ethanol directly from delignified oak wood under semi-aerobic liquid culture conditions. The transition from aerobic conditions (biological delignification pretreatment) to semi-aerobic conditions (saccharification and fermentation) enabled the fermentation of wood by solely biological processes (Kamei et al 2012b). However, a low solids concentration of cellulosic substrates (2% w/w) was used in these studies.…”

Section: Introductionmentioning

confidence: 99%

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Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

Kamei¹,

Hirota²,

Meguro³

2014

BioResources

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A white-rot fungus, Phlebia sp. MG-60, was applied to the fermentation of high-solid loadings of unbleached hardwood kraft pulp (UHKP) without the addition of commercial cellulase. From 4.7% UHKP, 19.6 g L -1 ethanol was produced, equivalent to 61.7% of the theoretical maximum. The highest ethanol concentration (25.9 g L -1 , or 46.7% of the theoretical maximum) was observed in the culture containing 9.1% UHKP. The highest filter paper activity (FPase) was observed in the culture containing 4.7% UHKP, while the production of FPase in the 16.5% UHKP culture was very low. Temporarily removing the silicone plug from Erlenmeyer flasks, which relieved the pressure and allowed a small amount of aeration, improved the yield of ethanol produced from the 9.1% UHKP, which reached as high as 37.3 g L -1 . These results indicated that production of cellulase and ensuing saccharification and fermentation by Phlebia sp. MG-60 is affected by water content and benefits from a small amount of aeration.

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Section: Cbp Fermentation Of High-concentration Uhkpmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

Kamei¹,

Hirota²,

Meguro³

2014

BioResources

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“…This white-rot fungus was able to selectively degrade lignin, and directly produced ethanol from delignified oak wood under aerobic solid-state fermentation conditions. This group of researchers in another study (Kamei et al 2012b) . designed an integrated fungal fermentation process of unified aerobic delignification and anaerobic saccharification and fermentation of wood by this strain.…”

Section: Flammulina Velutipesmentioning

confidence: 96%

“…designed an integrated fungal fermentation process of unified aerobic delignification and anaerobic saccharification and fermentation of wood by this strain. Transition from aerobic conditions (biological delignification pretreatment) to semi-aerobic conditions (saccharification and fermentation) enabled the fermentation of wood solely by biological processes (Kamei et al 2012b). To optimize using higher concentrations of cellulosic substrates, Kamei et al (2014a) designed new experiments with high concentrations (high-solid loadings) of unbleached hardwood kraft pulp (2.0, 4.7, 9.1, and 16.5%w/w) for CBP ethanol production.…”

Section: Flammulina Velutipesmentioning

confidence: 99%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

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HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

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Production of Liquid Biofuels from Lignocellulosic Biomass

Singh

Sahni

Narang

2021

Energy

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Integrated delignification and simultaneous saccharification and fermentation of hard wood by a white-rot fungus, Phlebia sp. MG-60

Cited by 76 publications

References 21 publications

Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

Production of Liquid Biofuels from Lignocellulosic Biomass

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