Integrated control of green mold to reduce chemical treatment in post-harvest citrus fruits

Moretto, Cristiane; Cervantes, Antonio Lucas Lima; Filho, Antonio Batista; Kupper, Kátia Cristina

doi:10.1016/j.scienta.2013.11.019

Cited by 27 publications

(17 citation statements)

References 20 publications

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“…Por outro lado, a combinação do tratamento térmico (52 °C/ 2 min) com os isolados de levedura ACB-K1 e ACB-CR1 (Saccharomyces cerevisiae) aumentou a eficiência de controle de P. digitatum em frutos de lima ácida Tahiti e tangor Murcott, respectivamente (Moretto et al, 2014). Shirra (2005) verificou controle de bolor verde em frutos cítricos, quando se combinou os produtos químicos imazalil e/ou thiabendazole nas doses de 50 mg L -1 com o tratamento térmico (50 °C).…”

Section: Produtos Naturaisunclassified

“…Klein & Kupper (2018) verificaram que a adição de sulfato de amônio (1%), como fonte de nitrogênio em meio batata-dextrose-ágar para cultivo da levedura, estimulou a produção de biofilme pelo microrganismo, aumentando a atividade antagônica contra o fungo G. citri-aurantii, sendo este o primeiro relato de uma correlação positiva entre o aumento na quantidade de biofilme produzido por A. pullulans, com o aumento da atividade antagônica.Medina-Córdova et al (2018) demonstraram o potencial de Debaryomyces hansenii em minimizar a presença do bolor azul nos frutos de limas e da podridão azeda em uvas, enquanto queMoretto et al (2014) relataram um controle de 100% do bolor verde (P. digitatum), quando frutas de lima ácida Tahiti foram tratadas com meia dose de imazalil associada com a levedura Saccharomyces cerevisiae.Alguns produtos à base de leveduras já são comercializados, como a formulação a base de Metschnikowia fructicola, produto denominado Shemer  e utilizado comercialmente em Israel para o controle de doenças pós-colheita de batata-doce, uva, morango, pimenta e cenoura(Droby et al, 2009;Kurtzman & Droby, 2001). Na Espanha, estão disponíveis os bioprodutos Pantovital  (Pantoea agglomerans) e Candifruit™ (Candida sake)(Usall et al, 2009).…”

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Alternativas de controle de doenças de pós-colheita em citros

Moura¹,

Moretto²,

Machado³

et al. 2019

Citrus Res. Technol.

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Section: Produtos Naturaisunclassified

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Alternativas de controle de doenças de pós-colheita em citros

Moura¹,

Moretto²,

Machado³

et al. 2019

Citrus Res. Technol.

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“…10,11 In addition, biocontrol agents can combat fungal pathogens using multiple modes of action, making the development of resistance by pathogens unlikely. 12 Over the past two decades, a large number of candidate biocontrol agents, including Bacillus subtilis, 13 Pseudomonas spp., 14 Kloeckera apiculate, 15,16 Pichia membranefaciens, 1 Hanseniaspora uvarum 17 and Rhodosporidium paludigenum, 18 have been isolated from plant rhizosphere soil, fruits and leaf surfaces and reported to be effective at managing fungal diseases of postharvest citrus fruits. Pseudomonas fluorescens is widely distributed in nature, and one of the most valuable biocontrol and plant growth-promoting rhizobacteria.…”

Section: Introductionmentioning

confidence: 99%

Potential modes of action of Pseudomonas fluorescens ZX during biocontrol of blue mold decay on postharvest citrus

Wang

Mei

et al. 2019

J Sci Food Agric

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BACKGROUND The application of chemical fungicides is currently the main method for the control of postharvest decay of fruits and vegetables. However, public concern has been growing towards the effect of fungicides on food safety, human health and environmental pollution. Thus, interest in microbial biocontrol agent development has grown, such agents being considered both safe and environmentally friendly. Pseudomonas fluorescens is widely distributed in nature, and one of the most valuable biocontrol and plant growth‐promoting rhizobacteria. In this study, the efficacy and the potential associated modes of action of P. fluorescens ZX against Penicillium italicum on oranges (Citrus sinensis Osbeck) were investigated. RESULTS The application of P. fluorescens ZX significantly reduced blue mold lesion size and incidence in comparison to the control, where P. fluorescens ZX was effective when applied preventatively but not curatively. In dual cultures, treatment with cell‐free autoclaved cultures or culture filtrate had a limited capacity to suppress P. italicum, while P. italicum was inhibited by bacterial fluid and bacterial suspension with living cells in vitro. The P. fluorescens ZX isolate displayed protease, but not chitinase, glucanase or cellulose, activity, and produced siderophores and volatile organic compounds with antifungal abilities. Competition tests showed P. fluorescens ZX could use fructose, sucrose, aspartic acid, threonine, serine, glycine, valine, lysine and proline better than P. italicum. Furthermore, an effective biofilm that peaked after a 24‐hour incubation at 30 °C was formed by the P. fluorescens ZX isolate. Light microscopy and scanning electron microscopy observations indicate the P. fluorescens ZX isolate could not undergo direct parasitism or hyperparasitism. CONCLUSIONS Competition for nutrients and niches, biofilm formation, inhibition of spore germination and mycelial growth, and production of inhibitory metabolites may play important roles in P. fluorescens ZX antagonism of P. italicum. © 2019 Society of Chemical Industry

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“…e biological control of major postharvest pathogens for citrus was reported by all kinds of microbial antagonists such as Bacillus subtilis [15,16], Pseudomonas spp. [17], Debaryomyces hansenii [18], Kloeckera apiculate [13], Candida membranifaciens [6], and so forth.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens

Wang

Jiang

Chen

et al. 2018

Journal of Food Quality

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The effectiveness of the bacteria antagonist Pseudomonas fluorescens to control green mold caused by Penicillium digitatum on oranges (Citrus sinensis Osbeck, cv. Jincheng) and the possible modes of action were evaluated. Whether in vitro or in vivo, treatments with cell-free autoclaved cultures or culture filtrate had limited capacity to suppress P. digitatum, while P. digitatum was significantly inhibited by bacterial fluid (P. fluorescens in the nutrient broth liquid medium) and bacterial suspension (P. fluorescens in sterile distilled water) with living cells. There was a positive relationship between the concentration of P. fluorescens in bacterial suspension and its biological efficacy. In addition, P. fluorescens was effective when applied preventatively but not when applied curatively. In the inoculated wounds, the population of P. fluorescens was an approximately 28- and 34-fold increase after being incubated at 20°C for 8 d and at 4°C for 16 d, respectively, and P. digitatum could effectively stimulate the growth and reproduction of P. fluorescens. Moreover, P. fluorescens was able to inhibit spore germination and germ tube elongation of P. digitatum as well as induce resistance on citrus peel by increasing the chitinase (CHI) activity and advancing the activities peaks of β-1,3-glucanase (GLU), peroxidase (POD), and phenylalanine ammonia lyase (PAL). All of these results support the potential application of P. fluorescens against green mold on postharvest citrus.

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Integrated control of green mold to reduce chemical treatment in post-harvest citrus fruits

Cited by 27 publications

References 20 publications

Alternativas de controle de doenças de pós-colheita em citros

Alternativas de controle de doenças de pós-colheita em citros

Potential modes of action of Pseudomonas fluorescens ZX during biocontrol of blue mold decay on postharvest citrus

Biocontrol of Penicillium digitatum on Postharvest Citrus Fruits by Pseudomonas fluorescens

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