Integrated analysis of long noncoding RNA‐associated competing endogenous RNA network in periodontitis

Li, S; Liu, Xiangqiong; Li, H; Pan, Hongying; Acharya, Aneesha; Deng, Yupei; Ye, Yu; Haak, Rainer; Schmidt, Jana; Schmalz, Gerhard; Ziebolz, Dirk

doi:10.1111/jre.12539

Cited by 50 publications

(50 citation statements)

References 47 publications

(54 reference statements)

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“…There are growing studies that suggest lncRNAs to play a pivotal role in disease pathogenesis by acting as ceRNAs to modulate the gene expression through sponging miRNAs [24,25]. Previous evidence pointed out that miR-142-3p is targeted by FGD5-AS1 as a part of the ceRNA network [11]. Moreover, miR-142-3p is considered as a mediator of inflammation and functions in regulating proinflammatory signalling pathways [26,27].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have pointed out that lncRNAs serving as competitive endogenous RNAs (ceRNAs) and sponging miRNAs in various diseases [12][13][14]. For example, FGD5-AS1 lncRNA, as a ceRNA, was proved to target miR-142-3p [11]. Based on these data, we investigated whether FGD5-AS1 regulates LPS-induced PDLC injury via sponging miR-142-3p.…”

Section: Introductionmentioning

confidence: 96%

“…Recently, endogenous long noncoding RNAs (lncRNAs) with more than 200 nucleotides have possessed extensive attention owing to their important roles in a large-scale biological processes [7][8][9]. Moreover, an increasing number of studies have revealed several lncRNAs to be abnormally expressed in chronic periodontitis (CP), thus playing a crucial role in periodontitis pathogenesis [10,11]. However, the exact roles of differentially expressed key lncRNAs in periodontitis pathogenesis have not been completely understood.…”

Section: Introductionmentioning

confidence: 99%

“…However, the exact roles of differentially expressed key lncRNAs in periodontitis pathogenesis have not been completely understood. Highthroughput RNA sequencing and microarray data on periodontitis have revealed the abnormal expression of lncRNA FGD5-AS1 to be associated with CP [11]. However, these data require verification through physiological experiments including the role of lncRNA FGD5-AS1 and its impact of action.…”

Section: Introductionmentioning

confidence: 99%

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Abnormal expression of long noncoding RNA FGD5-AS1 affects the development of periodontitis through regulating miR-142-3p/SOCS6/NF-κB pathway

Chen

Ze-dong

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Periodontitis refers to the inflammation of gums and the surrounding structures and caused by a bacterial infection. The infection occurs owing to poor oral hygiene and could destroy the bone and the gum over time if left untreated. The present study identified the involvement of a key long noncoding RNA (lncRNA), i.e. FGD5-AS1, in the pathogenesis of periodontitis by assessing its expression in the gingival tissues of patients diagnosed with chronic periodontitis (CP). Overexpression of FGD5-AS1 in primary human periodontal ligament cells (PDLCs) significantly reduced the lipopolysaccharide (LPS)induced periodontitis, whereas its suppression aggravated this injury. Moreover, the miR-142-3p was markedly expressed in the gingival samples of patients diagnosed with CP and LPS-induced PDLCs. We found that the FGD5-AS1-mediated reduction in the inflammation was mediated through downside regulation of miR-142-3p, as evident from the upregulation of SOCS6, a target gene of miR-142-3p. Furthermore, the association between FGD5-AS1 and NF-jB pathway was detected. FGD5-AS1 was found to protect against LPS-stimulated PDLC injury through restraining the NF-jB signals. Based on these findings, we conclude that up-regulation of lncRNA FGD5-AS1 could protect against periodontitis via regulating the miR-142-3p/SOCS6/NF-jB signals. Therefore, the FGD5-AS1/miR-142-3p/SOCS6 axis may act as an important indicator in explaining the pathogenesis of periodontitis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Abnormal expression of long noncoding RNA FGD5-AS1 affects the development of periodontitis through regulating miR-142-3p/SOCS6/NF-κB pathway

Chen

Ze-dong

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…LncRNAs, miRNAs, and mRNAs interact with each other to form regulatory competitive endogenous RNA networks [6]. Recent studies have shown that there are signi cant differences in the expression pro le of miRNAs and lncRNAs between in ammatory and healthy gingival tissue in patients with periodontitis [7]. However, the ceRNA network involved in the pathogenesis of peri-implantitis has not been reported by scholars.…”

Section: Introductionmentioning

confidence: 99%

Development of An Immunogenomic Landscape For The Competing Endogenous RNAs Network of Peri-Implantitis

Zheng

Gong

et al. 2020

Preprint

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Background: Peri-implantitis is an inflammation that occurs around the implant, resulting in varying degrees of inflammatory damage to the soft and hard tissues. The characteristic criterion is the loss of the supporting bone in an inflammatory environment. However, the specific mechanisms and biomarkers involved in peri-implantitis remain to be further studied. Recently, competing endogenous RNAs (ceRNA) and immune microenvironment have been found to play a more important role in the inflammatory process. In our study, we analyzed the expression of immune related microRNAs (miRNAs), long noncoding RNAs (lncRNAs) and message RNAs (mRNAs) in peri-implantitis by analyzing GSE33774 and GSE57631.Methods: In this study, we explored the expression profile data of immune-related lncRNAs, miRNAs and mRNAs, and constructed immune-related ceRNA network involved in the pathogenesis of peri-implantitis. In addition, the CIBERSORT was used to evaluate the content of immune cells in normal tissues and peri-implantitis to detect the immune microenvironment of peri-implantitis.Results: In the analysis, the corresponding lncRNAs, 14 DElncRNAs, 16 DEmiRNAs, and 18 DEmRNAs were used to establish an immune related ceRNA network constructed and the immune infiltration patterns associated with peri-implantitis was discovered. Through the mutual verification of the two datasets, we found that GSK3B and miR-1297 may have important significance in the immune microenvironment and pathogenesis of peri-implantitis and GSK3B was closely related to four types of immune cells, which needs to be further proved by later experiments. Conclusions: Through immune-related ceRNA network, IRGs and immune cell infiltration can further comprehensively understand the pathogenesis of peri-implantitis, which built up an immunogenomic landscape with clinical significance for peri-implantitis.One-sentences summary: GSK3B and miR-1297 may have important significance in the immune microenvironment and pathogenesis of peri-implantitis.

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Blood and tissue levels of lncRNAs in periodontitis

Sayad

Ghafouri‐Fard

Shams

et al. 2020

Journal Cellular Physiology

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Periodontitis is a complex disorder that affects a large number of human beings from different ethnic groups. This condition has been associated with dysregulation of a number of genes, among them are long noncoding RNAs (lncRNAs). In the current study, we assessed the expression of four lncRNAs (BDNF‐AS, MIAT, MIR137HG, and PNKY) as well as BDNF in the peripheral blood and gingival tissues obtained from patients with periodontitis and healthy subjects. The expression of BDNF was significantly lower in blood samples of male patients with periodontitis compared with male controls (posterior β of RE = −4.754, p = .048). However, there was no significant difference in the expression of BDNF in tissue samples from the cases and controls. The expression of BDNF‐AS was significantly lower in the tissue samples of patients compared with control tissue samples (posterior β of RE = −2.151, p = .019). Such an expression difference was detected between male subgroups as well (posterior β of RE = −3.679, p = .009). However, expression of this lncRNA was not different in blood samples obtained from patients compared with healthy subjects. The expression of PNKY was significantly higher in tissue samples obtained from female patients compared with sex‐matched controls (posterior β of RE = 6.23, p = .037). Blood levels of this lncRNA were not different between cases and controls. There was no significant difference either in the tissue expression or in blood expression of MIR137HG or MIAT between cases and controls. The current study indicates the putative role of BDNF, BDNF‐AS, and PNKY in the pathophysiology of periodontitis and potentiates these genes as candidates for functional studies.

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Integrated analysis of long noncoding RNA‐associated competing endogenous RNA network in periodontitis

Cited by 50 publications

References 47 publications

Abnormal expression of long noncoding RNA FGD5-AS1 affects the development of periodontitis through regulating miR-142-3p/SOCS6/NF-κB pathway

Abnormal expression of long noncoding RNA FGD5-AS1 affects the development of periodontitis through regulating miR-142-3p/SOCS6/NF-κB pathway

Development of An Immunogenomic Landscape For The Competing Endogenous RNAs Network of Peri-Implantitis

Blood and tissue levels of lncRNAs in periodontitis

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