Intake of a Milk-Based Wolfberry Formulation Enhances the Immune Response of Young-Adult and Aged Mice

Vidal, Karine; Benyacoub, Jalil; Sánchez-García, José Luis; Foata, Francis; Segura-Roggero, Iris; Serrant, Patrick; Moser, Mireille; Blum, Stéphanie

doi:10.1089/rej.2009.0895

Cited by 18 publications

(21 citation statements)

References 32 publications

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“…Rats were immunized as described by Vidal et al 26 The T cell-dependent humoral response (antigen-specific antibody production) was measured after immunization of rats with KLH, an innocuous protein isolate that generates a strong T cell-mediated antibody response and has been used extensively in animals. All rats were immunized on day 15 of the study with a subcutaneous injection of 250 lg of KLH emulsified in 1% Imject Alum.…”

Section: Immunization Protocolmentioning

confidence: 99%

“…The response was calculated as the difference in ear thickness of the rats before and after challenge with a recall antigen. DTH response assay was performed according to the method of Vidal et al 26 In brief, on day 22 of the study (i.e., 7 days after KLH immunization), the thickness of the ears was measured with a micrometer, and immediately thereafter the DTH response was elicited by intradermal injection of KLH (20 lL of 1lg/mL in phosphate-buffered saline Blood sampling and hematologic index/biochemical parameter assays…”

Section: Dth Response Assaymentioning

confidence: 99%

“…The levels of KLH-specific IgG, IgG 1 , and IgG 2a in the blood were measured by an enzyme-linked immunosorbent assay (ELISA) according to the method of Vidal et al 26 with a slight modification. Briefly, microtiter plates were coated with KLH (50 lL/well at 5 lg/mL for total IgG and IgG 1 and 10 lg/mL for IgG 2a ), incubated at 37°C for 2 hr and blocked with 5% FCS for 1 hr at 37°C.…”

Section: Klh-specific Igg Concentration Assaymentioning

confidence: 99%

“…[15][16][17] Resveratrol (3,5,40-trihydroxystilbene), a polyphenol, is a bioactive substance with multiple functions that occurs naturally in several plant species, including grapevines and berries. Accumulating data have suggested that resveratrol has anticarcinogenic, antiinflammatory, antimicrobial, antiviral, and antioxidant properties that might be relevant to chronic diseases and/or longevity in humans.…”

Section: Introductionmentioning

confidence: 99%

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Dietary Intake of Resveratrol Enhances the Adaptive Immunity of Aged Rats

Yuan

Lu²,

Zhang³

et al. 2012

Rejuvenation Research

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It is well known that immune response declines with aging. Resveratrol, a polyphenol that occurs naturally in several plant species including grapevines and berries, has been shown to have potent antiaging and healthpromoting activities. However, the mechanism underlying these activities remains largely unknown. Here we clearly demonstrate that: (1) Dietary intake of resveratrol induced a significant increase in T helper cells (CD4 + ) in middle-aged (12 months old) and aged (21 months old) Wistar male rats; (2) resveratrol supplementation considerably increased the delayed-type hypersensitivity response, a T cell-mediated immune response, in aged rats; and (3) reveratrol supplementation remarkably promoted the production of total anti-keyhole limpet hemocyanin (KLH) immunoglobulin G (IgG), anti-KLH IgG 1 , and anti-KLH IgG 2a in aged rats without disturbing immune homeostasis. These data together indicate that resveratrol is capable of counteracting immunosenescence, thereby leading to rejuvenation. In practice, resveratrol can be useful to help the elderly generate an improved response to vaccine with stronger humoral and cell-mediated immune responses.

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Section: Immunization Protocolmentioning

confidence: 99%

Section: Dth Response Assaymentioning

confidence: 99%

Section: Klh-specific Igg Concentration Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Dietary Intake of Resveratrol Enhances the Adaptive Immunity of Aged Rats

Yuan

Lu²,

Zhang³

et al. 2012

Rejuvenation Research

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“…A specially formulated wolfberry product called Lacto-Wolfberry (LWB), 8 which is made by homogenizing wolfberries in skimmed milk, has been shown to greatly enhance bioavailability of zeaxanthin (18). Recent studies have shown that dietary supplementation with wolfberry can enhance immune responses in both animals and humans (19,20). Furthermore, LWB supplementation (13.7 g/d) was reported to increase specific antibody response to the influenza vaccine in healthy free-living elderly individuals (21).…”

Section: Introductionmentioning

confidence: 99%

Dietary Supplementation with Lacto-Wolfberry Enhances the Immune Response and Reduces Pathogenesis to Influenza Infection in Mice,

Ren

et al. 2012

The Journal of Nutrition

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Despite the availability of vaccines, influenza is a considerable public health problem, which emphasizes the need for development of additional strategies to enhance host defense against influenza. Wolfberry, or goji berry, long used as a medicinal food in China, has recently been shown to improve immune response in mice. Because immune response plays a key role in the body's defense against pathogens, we hypothesized that wolfberry may increase host resistance to influenza infection by enhancing immune response. To test this hypothesis, we fed adult mice (4 mo old) a milk-based preparation of wolfberry called Lacto-Wolfberry (LWB) for 4 wk and then infected them with influenza A/Puerto Rico/8/34 (H1N1) while continuing the same experimental diets. Viral titer, lung pathology, and immune response were determined at different time points postinfection. LWB supplementation prevented infection-induced weight loss and reduced lung pathology on days 6 and 9 postinfection (P < 0.05). LWB-fed mice showed overall, significantly higher concanavalin A-induced IL-2 production (P < 0.05). Furthermore, we found positive correlations between weight loss and lung viral titer, pathology score, TNFα, and IL-6 production as well as negative correlations with T cell proliferation and IL-2 production (all P ≤ 0.05). These results indicate that LWB supplementation can attenuate symptoms and pathology of influenza infection by decreasing inflammatory cytokines in lungs while enhancing systemic T cell-mediated function as measured by their ability to produce IL-2.

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Healthy ageing: the natural consequences of good nutrition—a conference report

et al. 2018

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Many countries are witnessing a marked increase in longevity and with this increased lifespan and the desire for healthy ageing, many, however, suffer from the opposite including mental and physical deterioration, lost productivity and quality of life, and increased medical costs. While adequate nutrition is fundamental for good health, it remains unclear what impact various dietary interventions may have on prolonging good quality of life. Studies which span age, geography and income all suggest that access to quality foods, host immunity and response to inflammation/infections, impaired senses (i.e., sight, taste, smell) or mobility are all factors which can limit intake or increase the body’s need for specific micronutrients. New clinical studies of healthy ageing are needed and quantitative biomarkers are an essential component, particularly tools which can measure improvements in physiological integrity throughout life, thought to be a primary contributor to a long and productive life (a healthy “lifespan”). A framework for progress has recently been proposed in a WHO report which takes a broad, person-centered focus on healthy ageing, emphasizing the need to better understand an individual’s intrinsic capacity, their functional abilities at various life stages, and the impact by mental, and physical health, and the environments they inhabit.

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Intake of a Milk-Based Wolfberry Formulation Enhances the Immune Response of Young-Adult and Aged Mice

Cited by 18 publications

References 32 publications

Dietary Intake of Resveratrol Enhances the Adaptive Immunity of Aged Rats

Dietary Intake of Resveratrol Enhances the Adaptive Immunity of Aged Rats

Dietary Supplementation with Lacto-Wolfberry Enhances the Immune Response and Reduces Pathogenesis to Influenza Infection in Mice,

Healthy ageing: the natural consequences of good nutrition—a conference report

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