Insulin-like growth factors I and II in the sole Solea senegalensis: cDNA cloning and quantitation of gene expression in tissues and during larval development

Funes, Victoria; Asensio, Esther; Ponce, Marian; Infante, Carlos; Cañavate, José Pedro; Manchado, Manuel

doi:10.1016/j.ygcen.2006.05.017

Cited by 50 publications

(16 citation statements)

References 32 publications

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“…Well defined expression windows of myogenic and growth-related genes during Senegalese sole ontogeny and larval development have been previously characterised, since different genes are activated or down-regulated at different stages of development [23, 32]. It is plausible that allometric differences in growth of specific tissues may influence the observed miRNA expression profiles.…”

Section: Discussionmentioning

confidence: 99%

Thermal plasticity of the miRNA transcriptome during Senegalese sole development

Campos

Sundaram

Valente³

et al. 2014

BMC Genomics

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BackgroundSeveral miRNAs are known to control myogenesis in vertebrates. Some of them are specifically expressed in muscle while others have a broader tissue expression but are still involved in establishing the muscle phenotype. In teleosts, water temperature markedly affects embryonic development and larval growth. It has been previously shown that higher embryonic temperatures promoted faster development and increased size of Senegalese sole (Solea senegalensis) larvae relatively to a lower temperature. The role of miRNAs in thermal-plasticity of growth is hitherto unknown. Hence, we have used high-throughput SOLiD sequencing to determine potential changes in the miRNA transcriptome in Senegalese sole embryos that were incubated at 15°C or 21°C until hatching and then reared at a common temperature of 21°C.ResultsWe have identified 320 conserved miRNAs in Senegalese sole, of which 48 had not been previously described in teleosts. mir-17a-5p, mir-26a, mir-130c, mir-206-3p, mir-181a-5p, mir-181a-3p and mir-199a-5p expression levels were further validated by RT- qPCR. The majority of miRNAs were dynamically expressed during early development, with peaks of expression at pre-metamorphosis or metamorphosis. Also, a higher incubation temperature (21°C) was associated with expression of some miRNAs positively related with growth (e.g., miR-17a, miR-181-5p and miR-206) during segmentation and at hatching. Target prediction revealed that these miRNAs may regulate myogenesis through MAPK and mTOR pathways. Expression of miRNAs involved in lipid metabolism and energy production (e.g., miR-122) also differed between temperatures. A miRNA that can potentially target calpain (miR-181-3p), and therefore negatively regulate myogenesis, was preferentially expressed during segmentation at 15°C compared to 21°C.ConclusionsTemperature has a strong influence on expression of miRNAs during embryonic and larval development in fish. Higher expression levels of miR-17a, miR-181-5p and miR-206-3p and down-regulation of miR-181a-3p at 21°C may promote myogenesis and are in agreement with previous studies in Senegalese sole, which reported enhanced growth at higher embryonic temperatures compared to 15°C. Moreover, miRNAs involved in lipid metabolism and energy production may also contribute to increased larval growth at 21°C compared to 15°C. Taken together, our data indicate that miRNAs may play a role in temperature-induced phenotypic plasticity of growth in teleosts.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-525) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Thermal plasticity of the miRNA transcriptome during Senegalese sole development

Campos

Sundaram

Valente³

et al. 2014

BMC Genomics

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“…The early detection of mRNAs in fish eggs is not rare and it has been associated to transcripts stored maternally that would enable the development in these first stages. However, genes that are highly expressed in embryos, such as insulin-like growth factor 2, generally play an essential role in embryogenesis [31,32]. Hepcidin null mice develop iron overload [33], while animals that overexpress hepcidin develop severe hypochromic anemia [34].…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization and gene expression analysis of four hepcidin genes in the redbanded seabream (Pagrus auriga)

Martín-Antonio¹,

Jiménez-Cantizano²,

Salas-Leiton³

et al. 2009

Fish & Shellfish Immunology

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“…IGFs (IGF-I and IGF-II) are key components of the somatotrophic axis acting as mediators of GH actions to regulate somatic growth [112,114]. igf-I is mainly expressed in liver whereas igf-II has a more ubiquitous expression in sole [40]. To exert their actions, IGFs bind to the same membrane receptor, the type 1 IGF receptor (IGF-IR).…”

Section: Effect Of Dxm On Hpt and Gh/igf Axesmentioning

confidence: 99%

Dexamethasone modulates expression of genes involved in the innate immune system, growth and stress and increases susceptibility to bacterial disease in Senegalese sole (Solea senegalensis Kaup, 1858)

Salas-Leiton¹,

Coste²,

Asensio³

et al. 2012

Fish & Shellfish Immunology

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Insulin-like growth factors I and II in the sole Solea senegalensis: cDNA cloning and quantitation of gene expression in tissues and during larval development

Cited by 50 publications

References 32 publications

Thermal plasticity of the miRNA transcriptome during Senegalese sole development

Thermal plasticity of the miRNA transcriptome during Senegalese sole development

Genomic characterization and gene expression analysis of four hepcidin genes in the redbanded seabream (Pagrus auriga)

Dexamethasone modulates expression of genes involved in the innate immune system, growth and stress and increases susceptibility to bacterial disease in Senegalese sole (Solea senegalensis Kaup, 1858)

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