Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging

Quirin, Sean; Peterka, Darcy S.; Yuste, Rafael

doi:10.1364/oe.21.016007

Cited by 93 publications

(84 citation statements)

References 30 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent camera-based approaches can also resolve the 3D structure of a sample by using special light shaping: microlens array [23] or phase mask [24] in front of the camera combined with extended depth of field [25] or holographic [24] illumination. Depth information is obtained from the camera images by computationally reversed transformation, which causes two major limitations.…”

Section: From Wide-field Microscopy To Singlepoint Two-photon Excitamentioning

confidence: 99%

Acousto-optical Scanning-Based High-Speed 3D Two-Photon Imaging In Vivo

Rózsa

Szalay

Katona

2016

Advanced Patch-Clamp Analysis for Neuroscientists

View full text Add to dashboard Cite

Recording of the concerted activity of neuronal assemblies and the dendritic and axonal signal integration of downstream neurons pose different challenges, preferably a single recording system should perform both operations. We present a three-dimensional (3D), high-resolution, fast, acousto-optic two-photon microscope with random-access and continuous trajectory scanning modes reaching a cubic millimeter scan range (now over 950 Â 950 Â 3000 μm 3 ) which can be adapted to imaging different spatial scales. The resolution of the system allows simultaneous functional measurements in many fine neuronal processes, even in dendritic spines within a central core (>290 Â 290 Â 200 μm 3 ) of the total scanned volume. Furthermore, the PSF size remained sufficiently low (PSF x < 1.9 μm, PSF z < 7.9 μm) to target individual neuronal somata in the whole scanning volume for simultaneous measurement of activity from hundreds of cells. The system contains new design concepts: it allows the acoustic frequency chirps in the deflectors to be adjusted dynamically to compensate for astigmatism and optical errors; it physically separates the z-dimension focusing and lateral scanning functions to optimize the lateral AO scanning range; it involves a custom angular compensation unit to diminish off-axis angular dispersion introduced by the AO deflectors, and it uses a high-NA, wide-field objective and high-bandwidth custom AO deflectors with large apertures. We demonstrate the use of the microscope at different spatial scales by first showing 3D optical recordings of action potential back propagation and dendritic Ca 2þ spike forward propagation in long dendritic segments in vitro, at near-microsecond temporal resolution. Second, using the same microscope we show volumetric random-access Ca 2þ imaging of spontaneous and visual stimulation-evoked activity from hundreds of cortical neurons in the visual cortex in vivo. The selection of active neurons in a volume that respond to a given stimulus was aided by the real-time data analysis and the 3D interactive visualization accelerated selection of regions of interest.

show abstract

Section: From Wide-field Microscopy To Singlepoint Two-photon Excitamentioning

confidence: 99%

Acousto-optical Scanning-Based High-Speed 3D Two-Photon Imaging In Vivo

Rózsa

Szalay

Katona

2016

Advanced Patch-Clamp Analysis for Neuroscientists

View full text Add to dashboard Cite

show abstract

“…The bead position is used to continually update the focal power of a Fresnel lens on the SLM, so that the bead is always in focus. This extends the range of the procedure beyond the depth of field of standard microscopes [7], yet does not require computational deconvolution [8].…”

Section: Introductionmentioning

confidence: 99%

Dynamic stereo microscopy for studying particle sedimentation

Lee¹,

Gibson²,

Phillips³

et al. 2014

Opt. Express

View full text Add to dashboard Cite

We demonstrate a new method for measuring the sedimentation of a single colloidal bead by using a combination of optical tweezers and a stereo microscope based on a spatial light modulator. We use optical tweezers to raise a micron-sized silica bead to a fixed height and then release it to observe its 3D motion while it sediments under gravity. This experimental procedure provides two independent measurements of bead diameter and a measure of Faxén's correction, where the motion changes due to presence of the boundary.

show abstract

“…In particular, SLMs are increasingly used with infrared femtosecond pulsed lasers to implement patterned two-photon excitation for either multisite neuronal imaging [3][4][5] or multisite photoactivation [6][7][8], to either record or manipulate neural activity, using calcium indicators or light sensitive opsins, respectively. These two-photon approaches afford deeper tissue penetration, crucial for in vivo applications, but are often laser power limited since the signal at each simultaneous site decreases quadratically instead of linearly with the total number of sites.…”

Section: Introductionmentioning

confidence: 99%

Extended Field-of-view and Increased-signal 3D Holographic Illumination with Time-division Multiplexing

Yang

Allen

Kauvar

et al. 2016

Imaging and Applied Optics 2016

View full text Add to dashboard Cite

Phase spatial light modulators (SLMs) are widely used for generating multifocal three-dimensional (3D) illumination patterns, but these are limited to a field of view constrained by the pixel count or size of the SLM. Further, with two-photon SLM-based excitation, increasing the number of focal spots penalizes the total signal linearly-requiring more laser power than is available or can be tolerated by the sample. Here we analyze and demonstrate a method of using galvanometer mirrors to timesequentially reposition multiple 3D holograms, both extending the field of view and increasing the total time-averaged two-photon signal. We apply our approach to 3D two-photon in vivo neuronal calcium imaging.

show abstract

Instantaneous three-dimensional sensing using spatial light modulator illumination with extended depth of field imaging

Cited by 93 publications

References 30 publications

Acousto-optical Scanning-Based High-Speed 3D Two-Photon Imaging In Vivo

Acousto-optical Scanning-Based High-Speed 3D Two-Photon Imaging In Vivo

Dynamic stereo microscopy for studying particle sedimentation

Extended Field-of-view and Increased-signal 3D Holographic Illumination with Time-division Multiplexing

Contact Info

Product

Resources

About