Insights on the interactions of human serum albumin with three natural phenylethanoid glycosides that inhibit HeLa cells proliferation

Huang, Yaoxin; Yang, Zhao; Chen, Ping; Zhao, Zhongxiang; Chen, Lin; Chang, Zhu; Wu, Aizhi

doi:10.1016/j.molstruc.2021.132050

Cited by 7 publications

(8 citation statements)

References 32 publications

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“…29 Results were further confirmed by spectroscopic analysis and computational approaches. Similar surface morphology changes were also observed in the presence of three natural phenylethanol glycosides (acteoside, cistanoside F, and decaffeoylacteoside), and hydrophobic and electrostatic forces contributed to the binding interaction 30 (Fig. 2A).…”

Section: Topographical Imaging Of Protein-drug Interactionsupporting

confidence: 70%

Novel perspective for protein–drug interaction analysis: atomic force microscope

Wang

2023

Analyst

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Section: Topographical Imaging Of Protein-drug Interactionsupporting

confidence: 70%

Novel perspective for protein–drug interaction analysis: atomic force microscope

Wang

2023

Analyst

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“…Previous studies also demonstrated that phenylethanoid glycosides have strong chemical activities to bind with proteins. 35,37 Moreover, the calculated ΔE value supported the observed trend of biological activities for the interaction of kusaginin with BSA. Taken together, the computational investigations confirmed the effective binding of kusaginin with BSA and formed a stable kusaginin-BSA complex.…”

Section: Discussionsupporting

confidence: 72%

“…The fluorescence emission intensity of BSA was decreased considerably with the successive addition of kusaginin, indicating that kusaginin could robustly quenched the intrinsic fluorescence of BSA. This effect was also seen in the other tested phenolic compounds 35 . BSA has three structurally distinct domains (I‐III), each domain is composed of two subdomains (A and B), and almost all the hydrophobic amino acid residues are buried in the hydrophobic cavities.…”

Section: Discussionmentioning

confidence: 56%

“…This effect was also seen in the other tested phenolic compounds. 35 BSA has three structurally distinct domains (I-III), each domain is composed of two subdomains (A and B), and almost all the hydrophobic amino acid residues are buried in the hydrophobic cavities. Thus, we speculated that the binding position of kusaginin to BSA may be located within this hydrophobic pocket, and addition of kusaginin changed the polarity of this hydrophobic microenvironment and caused the conformational changes of BSA.…”

Section: Discussionmentioning

confidence: 99%

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Insights into the interaction between the kusaginin and bovine serum albumin: Multi‐spectroscopic techniques and computational approaches

Huang

Chen

2022

J of Molecular Recognition

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Kusaginin, as a phenylethanoid glycoside, which has exhibited wide antioxidant and antimicrobial properties. The molecular mechanism underlying the broad biological activities of kusaginin has not yet been well documented. In this paper, the interaction of kusaginin with bovine serum albumin (BSA) has been explored by fluorescence spectra, UV‐vis absorption spectra, and circular dichroism (CD) spectra along with computational approaches. The fluorescence experiments showed that kusaginin could strongly quench the intrinsic fluorescence of BSA through both dynamic and static quenching mechanisms. The thermodynamic analysis suggested that hydrophobic force was the main force in stabilizing the BSA‐kusaginin complex. In addition, conformation changes of BSA were observed from three‐dimensional and synchronous fluorescence spectra, UV spectra, and CD spectra under experimental conditions. All these experimental results have been complemented and validated by the molecular docking and dynamic simulation studies, which revealed that kusaginin was bound on the hydrophobic cavity in subdomain IIA of BSA and formed a stable BSA‐kusaginin complex. Finally, density functional theory (DFT) calculation further implied that hydrogen bonds also support stabilizing the BSA‐kusaginin complex. This research may aid in understanding the pharmacological characteristics of kusaginin and provide a vital reference modeling for the design of analogues drugs.

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“…Other substances isolated from the leaves and twigs of Paulownia Clone in vitro 112 include small amounts of dicaffeoylacteoside, 1-O-caffeoyl-6-O-alpha-rhamnopyranosyl-beta-glycopyranoside, 3-(4-Hydroxyphenyl)-1,2-propanediol4′-O-glucoside, campneoside I, acetyl acteoside (tubuloside B), epimeredinoside A, and didehydroxyacteoside [ 43 ]. Dicaffeoylacteoside showed good radical-scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical (SC 50 = 19.6 ± 1.4 μM) and was cytotoxic toward SK-LU-1, MCF7, HepG2, and HeLa cancer cell lines [ 126 , 127 ]. Tubuloside B demonstrated neuroprotective properties.…”

Section: Chemical Content Of Paulownia Clone In Vitro 112 and Its Bio...mentioning

confidence: 99%

Paulownia Organs as Interesting New Sources of Bioactive Compounds

Sławińska

Zając

Olas

2023

IJMS

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Paulownia spp. is a genus of trees in the Paulowniaceae family. It is native to southeastern Asia (especially China), where it has been cultivated for decorative, cultural, and medicinal purposes for over 2000 years. Depending on taxonomic classification, there are 6 to 17 species of Paulownia; P. tomentosa, P. elongata, P. fortunei, and P. catalpifolia are considered the most popular. Nowadays, Paulownia trees are planted in Asia, Europe, North America, and Australia for commercial, medical, and decorative purposes. Lately, growing interest in Paulownia has led to the development of various hybrids, the best-known being Clone in vitro 112, Shan Tong, Sundsu 11, and Cotevisa 2. Paulownia Clone in vitro 112 is an artificially created hybrid of two species of Paulownia: P. elongata and P. fortunei. The present review of selected papers from electronic databases including PubMed, ScienceDirect, and SCOPUS before 15 November 2022 describes the phytochemical characteristics, biological properties, and economic significance of various organs from different Paulownia species and hybrids, including P. tomentosa, P. elongata, P. fortunei, and Paulownia Clone in vitro 112. Many compounds from Paulownia demonstrate various biological activities and are promising candidates for natural preparations; for example, the leaves of Clone in vitro 112 have anti-radical and anticoagulant potential. However, further in vivo studies are needed to clarify the exact mechanism of action of the active substances and their long-term effects.

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Insights on the interactions of human serum albumin with three natural phenylethanoid glycosides that inhibit HeLa cells proliferation

Cited by 7 publications

References 32 publications

Novel perspective for protein–drug interaction analysis: atomic force microscope

Novel perspective for protein–drug interaction analysis: atomic force microscope

Insights into the interaction between the kusaginin and bovine serum albumin: Multi‐spectroscopic techniques and computational approaches

Paulownia Organs as Interesting New Sources of Bioactive Compounds

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