Insights into the role of adipose‐derived stem cells: Wound healing and clinical regenerative potential

Zou, Ming-Li; Liu, Siyu; Sun, Ziyong; Wu, Junjie; Yuan, Zheng‐Dong; Teng, Ying-Ying; Feng, Yi; Yuan, Feng-Lai

doi:10.1002/jcp.30019

“…A biopsy report or a pathology report is other name for a histopathology report. Vitiligo can be diagnosed if the melanocytes and melanocyte granules decrease or even disappear under the microscope [26]. The major goal of this disease's treatment is to get the white plaques and skin back to normal.…”

Section: Discussionmentioning

confidence: 99%

Clinical Effect of Microneedle Injection Combined with Blood Transfusion in the Treatment of Severe Anemia Complicated with Vitiligo under Regenerative Medical Technology

He¹,

Gong

²

2022

BioMed Research International

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To explore the clinical efficacy of microneedle injection combined with blood transfusion in the treatment of severe anemia complicated with vitiligo based on regenerative medical technology and provide the theoretical basis for the adoption of microneedle technology, 60 patients with severe anemia complicated with vitiligo were selected as research objects. With 15 patients in each group, they were randomly assigned to the control group (calcipotriol ointment external application), observation group A (external application of moist exposed burn ointment (MEBO), observation group B (external application of MEBO combined with blood transfusion), and observation group C (microneedle injection of MEBO combined with blood transfusion). Blood indexes and plaque recovery of patients in different periods were detected. The total protein (TP) content in group C ( 62.3 ± 3.3 g/L and 64.3 ± 2.88 g/L) was remarkably higher than that in the control group ( 51.3 ± 3.17 g/L and 52.4 ± 3.17 g/L) and group A ( 52.6 ± 2.91 g/L and 51.8 ± 2.98 g/L)) at the 5th and 7th weeks after the treatment ( P < 0.05 ). The albumin (ALB) content in group C ( 42.9 ± 3.28 g/L and 45.3 ± 3.1 g/L) was signally higher than that in the control group ( 41.8 ± 3.44 g/L and 41.9 ± 3.23 g/L) and group A ( 41.3 ± 2.91 g/L and 42.1 ± 3.02 g/L) at the 5th and 7th weeks after the treatment, and the content was markedly higher than that in group B at 5th week ( P < 0.05 ). The wound healing rates of group C at the 3rd, 5th, and 7th weeks after the treatment (38.44%, 56.6%, and 90.23%) were greatly higher than those of the control group, group A, and group B ( P < 0.05 ). Besides, the wound healing rate of group B was higher than that of the control group and group A (40.3% and 75.8%) at the 5th and 7th weeks after the treatment ( P < 0.05 ). To sum up, based on regenerative medical technology, microneedle injection (microneedling is a derma roller process that pricks the skin with minuscule needles. The goal of the treatment is to develop new collagen and skin tissue, resulting in skin that is smoother, firmer, and more toned) combined with blood transfusion had a good therapeutic effect on patients with severe anemia complicated with vitiligo, which could manifestly improve the blood indexes and skin plaques of patients, with a good clinical adoption effect.

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“…The ASC-patch studied here essentially relies on the combined interaction of adipose-derived stem cells with gelatin. ASCs are known for their ability to enhance the healing process by secretion of soluble healing factors and extracellular vesicles [37][38][39][40]. Here we demonstrate that ASCs not only increase their regenerative potential when they grow within the patch, but are also locally concentrated.…”

Section: Discussionmentioning

confidence: 55%

Efficacy and safety of a patch containing adipose-derived stem cells for skin wound healing – results form a comprehensive pre-clinical evaluation program

Brembilla

¹

,

M

²

,

Lévigne

³

et al. 2022

Preprint

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Mesenchymal stem cell-based therapies are emerging as innovative approaches to treat chronic wounds. A common administration route used in clinical trials consists of local injections leading to uncontrolled/sub-optimal delivery. This study reports a comprehensive pre-clinical evaluation program on the mechanism of action, efficacy and safety of an easy-to-use patch that concentrates Adipose-derived Stem Cells (ASCs) in a clinical-grade sponge of porcine crosslinked-gelatin. ASCs were prepared from the fat of ischemic patients. Transcriptome and proteome of ASC-patches and ASC monolayers were assessed by microarrays, bio-arrays and mass spectrometry. Tumorigenesis was investigated in immunosuppressed mice according to the European Pharmacopeia. Angiogenesis was assessed in vivo in the chick chorioallantoic membrane model. Efficacy of the ASC-patch was tested in a rat model of ischemic full-thickness skin defect. Cell stability was assessed by luminescence using ASC-patches generated from ASCs stably transduced with firefly luciferase. ASCs from ischemic patients upregulated the transcription of multiple genes involved in skin wound healing when cultured within the ASC-patch formulation. The patch was not only a concentrator, but also a reservoir of both ASC-derived regenerative factors and sponge-derived soluble fragments having healing capacity. The secretome of the ASC-patch promoted dermal fibroblast survival and epidermal epithelialization. No tumor formation was observed in immunodeficient Nude mice subcutaneously transplanted with the ASC-patch. Transplanted patches were early invaded by new vessels in vivo, and a marked angiogenesis was confirmed in two independent animal models. Finally, ASC-patches prepared from syngeneic rats promoted faster healing and re-vascularization of full-thickness skin defects in a rat animal model. Of note, ASCs were viable and locally stable for at least two weeks in vivo. We provide here compelling pre-clinical evidence that patches concentrating ASCs within crosslinked gelatin may represent a convenient and effective tool for the management of chronic wounds.

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“…Notably, adipocyte precursor cells and mature adipocytes are involved in a variety of regenerative and pathophysiologic processes in the skin, including hair follicle (HF) regeneration and fibroblast regeneration after injury. 44 An emerging theme that has attracted attention involves the influence of hair follicles on the myofibroblast reduction and maturation of adipocyte lineage cells (Figure 2 ). A well‐known finding is the relationship between the HF growth cycle and oscillations in the thickness of the dermal adipocyte layer.…”

Section: Experimental Myofibroblast‐to‐adipocyte Reprogrammingmentioning

confidence: 99%

“…In contrast, a number of studies have focused on the role of dermal adipose tissue in skin regeneration over the past few years. Notably, adipocyte precursor cells and mature adipocytes are involved in a variety of regenerative and pathophysiologic processes in the skin, including hair follicle (HF) regeneration and fibroblast regeneration after injury 44 . An emerging theme that has attracted attention involves the influence of hair follicles on the myofibroblast reduction and maturation of adipocyte lineage cells (Figure 2).…”

Section: Experimental Myofibroblast‐to‐adipocyte Reprogrammingmentioning

confidence: 99%

Novel prospects for scarless wound healing: The roles of myofibroblasts and adipocytes

Teng

¹

,

Zou

²

,

Zhou

³

et al. 2022

J Cellular Molecular Medi

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Disturbances or defects in the process of wound repair can disrupt the delicate balance of cells and molecules necessary for complete wound healing, thus leading to chronic wounds or fibrotic scars. Myofibroblasts are one of the most important cells involved in fibrotic scars, and reprogramming provides a potential avenue to increase myofibroblast clearance. Although myofibroblasts have long been recognized as terminally differentiated cells, recent studies have shown that myofibroblasts have the capacity to be reprogrammed into adipocytes. This review intends to summarize the potential of reprogramming myofibroblasts into adipocytes. We will discuss myofibroblast lineage tracing, as well as the known mechanisms underlying adipocyte regeneration from myofibroblasts. In addition, we investigated different changes in myofibroblast gene expression, transcriptional regulators, signalling pathways and epigenetic regulators during skin wound healing. In the future, myofibroblast reprogramming in wound healing will be better understood and appreciated, which may provide new ideas for the treatment of scarless wound healing.

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Insights into the role of adipose‐derived stem cells: Wound healing and clinical regenerative potential

Cited by 15 publications

References 46 publications

Clinical Effect of Microneedle Injection Combined with Blood Transfusion in the Treatment of Severe Anemia Complicated with Vitiligo under Regenerative Medical Technology

Clinical Effect of Microneedle Injection Combined with Blood Transfusion in the Treatment of Severe Anemia Complicated with Vitiligo under Regenerative Medical Technology

Efficacy and safety of a patch containing adipose-derived stem cells for skin wound healing – results form a comprehensive pre-clinical evaluation program

Novel prospects for scarless wound healing: The roles of myofibroblasts and adipocytes

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