Insights into the Composition of a Co-Culture of 10 Probiotic Strains (OMNi BiOTiC® AAD10) and Effects of Its Postbiotic Culture Supernatant

Kienesberger, Bernhard; Obermüller, Beate; Singer, Georg; Arneitz, Christoph; Gasparella, Paolo; Klymiuk, Ingeborg; Horvath, Angela; Stadlbauer, Vanessa; Magnes, Christoph; Zügner, Elmar; López-García, Pablo; Trajanoski, Slave; Miekisch, Wolfram; Fuchs, Patricia; Till, Holger; Castellani, Christoph

doi:10.3390/nu14061194

Cited by 8 publications

(6 citation statements)

References 67 publications

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“…In another clinical study, this multi-strain probiotic positively influenced the gastrointestinal tract of patients with diarrhoea-predominated irritable bowel syndrome [120]. In another study, the multi-strain postbiotic supernatant of the dietary supplement OMNi-BiOTic AAD10 with similar composition exhibited positive antibacterial and antifungal effects in vitro [121].…”

Section: Discussionmentioning

confidence: 90%

The Antimicrobial Effect of Various Single-Strain and Multi-Strain Probiotics, Dietary Supplements or Other Beneficial Microbes against Common Clinical Wound Pathogens

et al. 2022

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The skin is the largest organ in the human body and is colonized by a diverse microbiota that works in harmony to protect the skin. However, when skin damage occurs, the skin microbiota is also disrupted, and pathogens can invade the wound and cause infection. Probiotics or other beneficial microbes and their metabolites are one possible alternative treatment for combating skin pathogens via their antimicrobial effectiveness. The objective of our study was to evaluate the antimicrobial effect of seven multi-strain dietary supplements and eleven single-strain microbes that contain probiotics against 15 clinical wound pathogens using the agar spot assay, co-culturing assay, and agar well diffusion assay. We also conducted genera-specific and species-specific molecular methods to detect the DNA in the dietary supplements and single-strain beneficial microbes. We found that the multi-strain dietary supplements exhibited a statistically significant higher antagonistic effect against the challenge wound pathogens than the single-strain microbes and that lactobacilli-containing dietary supplements and single-strain microbes were significantly more efficient than the selected propionibacteria and bacilli. Differences in results between methods were also observed, possibly due to different mechanisms of action. Individual pathogens were susceptible to different dietary supplements or single-strain microbes. Perhaps an individual approach such as a ‘probiogram’ could be a possibility in the future as a method to find the most efficient targeted probiotic strains, cell-free supernatants, or neutralized cell-free supernatants that have the highest antagonistic effect against individual clinical wound pathogens.

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Section: Discussionmentioning

confidence: 90%

The Antimicrobial Effect of Various Single-Strain and Multi-Strain Probiotics, Dietary Supplements or Other Beneficial Microbes against Common Clinical Wound Pathogens

et al. 2022

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“…Promisingly, the culture supernatant of B. fragilis appears to have similar effects, suppressing high expression of IL-1β and IL-6 induced by TNF-α and increasing the anti-inflammatory factor IL-10, whereas conversely promoting and superimposing the expression of IL-8 induced by TNF-a, indicating that the mechanisms by which the anti-inflammatory effects are exerted by B. fragilis itself and the metabolites might be distinct. A recent study by Bernhard 45 investigated the composition of 10 strains of probiotic bacteria (OMNi bio®AAD10) and the effect of their supernatant after cultivation. They have shown that the supernatant of these bacteria had excellent antibacterial and antifungal effects and promoted the growth of different bacteria in vivo.…”

Section: Discussionmentioning

confidence: 99%

Protective effects of a new generation of probiotic Bacteroides fragilis against colitis in vivo and in vitro

He,

Niu,

et al. 2023

Sci Rep

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Bacteroides fragilis, one of the potential next-generation probiotics, but its protective mechanism is not yet known. We aimed to characterize the anti-inflammatory effect of B. fragilisATCC25285 and to elucidate its mechanism through in vivo and in vitro experiments. An in vitro model of inflammation by induction of colonic cells with TNF-a, and co-cultured with B. fragilis to detect cell viability, apoptosis and invasive capacity. Furthermore, critical proteins of the TLR/NF-κB pathway and the inflammatory cytokines were measured. For animal trials, C57BL/6 J male mice were orally administered B. fragilis or PBS once daily for 21 days. Colitis was induced by drinking 2.5% DSS from days 0 to 7. The mice were weighed daily and rectal bleeding, stool condition and blood in the stool were recorded. We found that B. fragilis treatment alone was harmless and had no effect on cell viability or apoptosis. While predictably TNF-α decreased cell viability and increased apoptosis, B. fragilis attenuated this deterioration. The NF-κB pathway and inflammatory cytokines IL-6 and IL-1β activated by TNF-α were also blocked by B. fragilis. Notably, the metabolic supernatant of B. fragilis also has an anti-inflammatory effect. Animal studies showed that live B. fragilis rather than dead strain ameliorated DSS-induced colitis, as evidenced by weight loss, shortened colon length and enhanced barrier function. The colonic tissue levels of inflammatory cytokines (TNF-α, IL-1β, IL-6) were decreased and IL-10 was increased as a result of B. fragilis administration. In conclusion, B. fragilis ATCC25285 exhibited anti-inflammatory effects whether in vivo or in vitro, and it may be a potential probiotic agent for improving colitis.

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“…Promisingly, the culture supernatant of B. fragilis appears to have similar effects, suppressing high expression of IL-1β and IL-6 induced by TNF-α and increasing the anti-in ammatory factor IL-10, whereas conversely promoting and superimposing the expression of IL-8 induced by TNF-a, indicating that the mechanisms by which the anti-in ammatory effects are exerted by B. fragilis itself and the metabolites might be distinct. A recent study by Bernhard [55] investigated the composition of 10 strains of probiotic bacteria (OMNi bio®AAD10) and the effect of their supernatant after cultivation. They have shown that the supernatant of these bacteria had excellent antibacterial and antifungal effects and promoted the growth of different bacteria in vivo.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Bacteroides fragilis on TNF-α- Induced Inflammatory Changes in Human Colon Epithelial Cells and in Inflammatory Bowel Disease Mouse Model

Niu

et al. 2023

Preprint

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Bacteroides fragilis, one of the potential next-generation probiotics, but its protective mechanism is not yet known. We aimed to characterize the anti-inflammatory effect of B. fragilis and to elucidate its mechanism through in vivo and in vitro experiments. An in vitro model of inflammation by induction of colonic cells with TNF-a, and co-cultured with B. fragilis to detect cell viability, apoptosis and invasive capacity. Furthermore, critical proteins of the TLR/NF-κB pathway and the inflammatory cytokines were measured. For animal trials, C57BL/6J male mice were orally administered B. fragilis or PBS once daily for 21 days. Colitis was induced by drinking 2.5% DSS from days 0 to 7. The mice were weighed daily and rectal bleeding, stool condition and blood in the stool were recorded. We found that B. fragilis treatment alone was harmless and had no effect on cell viability or apoptosis. While predictably TNF-α decreased cell viability and increased apoptosis, B. fragilis attenuated this deterioration. The NF-κB pathway and inflammatory cytokines IL-6, IL-8 and IL-1β activated by TNF-α were also blocked by B. fragilis, which was likely mediated by TLR2. Animal studies showed that B. fragilis ameliorated DSS-induced colitis, as evidenced by weight loss, shortened colon length and enhanced barrier function. The colonic tissue levels of inflammatory cytokines (TNF-α, IL1β, IL6) were decreased and IL-10 was increased as a result of B. fragilisadministration. In conclusion, B. fragilisexhibited anti-inflammatory effects whether in vivo or in vitro, and it may be a potential probiotic agent for improving colitis.

show abstract

Insights into the Composition of a Co-Culture of 10 Probiotic Strains (OMNi BiOTiC® AAD10) and Effects of Its Postbiotic Culture Supernatant

Cited by 8 publications

References 67 publications

The Antimicrobial Effect of Various Single-Strain and Multi-Strain Probiotics, Dietary Supplements or Other Beneficial Microbes against Common Clinical Wound Pathogens

The Antimicrobial Effect of Various Single-Strain and Multi-Strain Probiotics, Dietary Supplements or Other Beneficial Microbes against Common Clinical Wound Pathogens

Protective effects of a new generation of probiotic Bacteroides fragilis against colitis in vivo and in vitro

Protective Effect of Bacteroides fragilis on TNF-α- Induced Inflammatory Changes in Human Colon Epithelial Cells and in Inflammatory Bowel Disease Mouse Model

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