Insights into Protein Structure and Dynamics by Ultraviolet and Visible Resonance Raman Spectroscopy

López-Peña, Ignacio; Leigh, Brian S.; Schlamadinger, Diana E.; Kim, Judy E.

doi:10.1021/acs.biochem.5b00514

Cited by 36 publications

(30 citation statements)

References 164 publications

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“…Table 1), the water bands are dominant for dilute protein solutions. Therefore, protein structure studies often utilize the resonance enhancement effect in the UV range [37] to increase the intensity of protein bands and take advantage of the low water absorptivity in the UV. The resonant effect of the Raman scattering in the UV region, referred to as UV resonance Raman (UVRR), is caused by the absorption of aromatic amino acids or the polypeptide backbone of proteins.…”

Section: Spectroscopic Methods and Their Applicability To Protein Monmentioning

confidence: 99%

A critical review of recent trends, and a future perspective of optical spectroscopy as PAT in biopharmaceutical downstream processing

2020

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As competition in the biopharmaceutical market gets keener due to the market entry of biosimilars, process analytical technologies (PATs) play an important role for process automation and cost reduction. This article will give a general overview and address the recent innovations and applications of spectroscopic methods as PAT tools in the downstream processing of biologics. As data analysis strategies are a crucial part of PAT, the review discusses frequently used data analysis techniques and addresses data fusion methodologies as the combination of several sensors is moving forward in the field. The last chapter will give an outlook on the application of spectroscopic methods in combination with chemometrics and model predictive control (MPC) for downstream processes.

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Section: Spectroscopic Methods and Their Applicability To Protein Monmentioning

confidence: 99%

A critical review of recent trends, and a future perspective of optical spectroscopy as PAT in biopharmaceutical downstream processing

2020

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“…The main advantage of VRRS is the ability to investigate different parts of a large protein molecule by tuning the excitation wave number into the absorption band of the chromophore of interest. In a recent paper [42], the application of VRRS in the study of the structure and dynamics of various proteins is discussed. [42] gives an excellent review of this field covering both visible and UV resonance Raman as well as cw and time-resolved versions of VRRS.…”

Section: Discussionmentioning

confidence: 99%

“…In a recent paper [42], the application of VRRS in the study of the structure and dynamics of various proteins is discussed. [42] gives an excellent review of this field covering both visible and UV resonance Raman as well as cw and time-resolved versions of VRRS.…”

Section: Discussionmentioning

confidence: 99%

What Is Vibrational Raman Spectroscopy: A Vibrational or an Electronic Spectroscopic Technique or Both?

Hassing¹

2020

Modern Spectroscopic Techniques and Applications

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Modern Raman spectroscopy covers several noninvasive reflection techniques for identification of molecules and investigation of molecular properties. All are based on the Raman effect, occurring when polarized laser light is inelastically scattered by a molecular sample. Vibrational Raman spectroscopy is the Raman technique most widely used in chemical analysis, and it is relevant for the characterization of molecules in solution, biomolecules, and solids (crystals and powders). In this chapter vibrational Raman spectroscopy is discussed under the headline: "What is vibrational Raman spectroscopy: a vibrational or an electronic spectroscopic technique, or both?" The answer to this question is gained through a discussion of different scattering situations, and it is demonstrated that the Raman technique is more than an alternative technique to infrared and near-infrared absorption spectroscopy. Starting with the Kramers-Heisenberg equation, the answer is obtained through a discussion of: State tensors and Raman tensors, nonresonance and resonance Raman scattering (RRS) excited with a single laser wavelength, and unpolarized and polarization-resolved RRS excited with variable laser wavelengths (RADIS), dispersive Raman vibrations as a tool for noninvasive detection of molecular color changes and that RADIS data are automatically born as three-dimensional multivariate data with high information contents.

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“…Raman scattering is a sensitive and selective method for studying molecular bonds, conformation, and environments, and it is also a promising non-invasive technique for biomedical applications [36]. RS proved to be a powerful technique that complements the structural studies of biomolecules and provides an understanding of the connection between structure and function.…”

Section: Introductionmentioning

confidence: 99%

Raman Scattering: From Structural Biology to Medical Applications

et al. 2020

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This is a review of relevant Raman spectroscopy (RS) techniques and their use in structural biology, biophysics, cells, and tissues imaging towards development of various medical diagnostic tools, drug design, and other medical applications. Classical and contemporary structural studies of different water-soluble and membrane proteins, DNA, RNA, and their interactions and behavior in different systems were analyzed in terms of applicability of RS techniques and their complementarity to other corresponding methods. We show that RS is a powerful method that links the fundamental structural biology and its medical applications in cancer, cardiovascular, neurodegenerative, atherosclerotic, and other diseases. In particular, the key roles of RS in modern technologies of structure-based drug design are the detection and imaging of membrane protein microcrystals with the help of coherent anti-Stokes Raman scattering (CARS), which would help to further the development of protein structural crystallography and would result in a number of novel high-resolution structures of membrane proteins—drug targets; and, structural studies of photoactive membrane proteins (rhodopsins, photoreceptors, etc.) for the development of new optogenetic tools. Physical background and biomedical applications of spontaneous, stimulated, resonant, and surface- and tip-enhanced RS are also discussed. All of these techniques have been extensively developed during recent several decades. A number of interesting applications of CARS, resonant, and surface-enhanced Raman spectroscopy methods are also discussed.

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Insights into Protein Structure and Dynamics by Ultraviolet and Visible Resonance Raman Spectroscopy

Cited by 36 publications

References 164 publications

A critical review of recent trends, and a future perspective of optical spectroscopy as PAT in biopharmaceutical downstream processing

A critical review of recent trends, and a future perspective of optical spectroscopy as PAT in biopharmaceutical downstream processing

What Is Vibrational Raman Spectroscopy: A Vibrational or an Electronic Spectroscopic Technique or Both?

Raman Scattering: From Structural Biology to Medical Applications

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