Insight into the tolerance, biochemical and antioxidative response in three moss species on exposure to BDE-47 and BDE-209

Zhao, Jingjing; Zhang, Meng; Zhang, Wei; Liu, Fuwen; Huang, Kai; Lin, Kuangfei

doi:10.1016/j.ecoenv.2019.06.044

Cited by 14 publications

(11 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T.cymbifolium POD activity was affected by 0.24 mg/L or more BDE-47, but no change was observed at difference concentrations of BDE-209. Similarities between SOD and POD suggest they work together to reduce harmful effects caused by ROS in H. plumaeforme and T.cymbifoliu (Zhao et al, 2019). In the case of CAT, highest activities in H. plumaeforme and T.cymbifolium were detected at 0.024 mg/L BDE-47, decreasing at higher concentrations.…”

Section: Studies In Vivo On Brominated Organic Compoundsmentioning

confidence: 90%

“…Toxicities of brominated compounds have been demonstrated using studies in vivo in different organisms (Table 3). BDE-47, BDE-209 and TBBPA have shown the greatest effects in growth and development, and, for this reason, effects on enzyme activities have been performed mostly in presence of these compounds (Darnerud et al, 2001;Feng et al, 2013;Yanhua Liu et al, 2018;Qiu et al, 2018;Ronisz et al, 2004;Sarkar et al, 2016;Sun et al, 2019;Tang et al, 2018;Xiong et al, 2018;Yu et al, 2018;Zhao et al, 2019). Large and small organisms have been used to explore their impact on enzymes, particularly those responsible for controlling oxidative stress caused by contaminants (SOD, GST, CAT, POD etc.)…”

Section: Studies In Vivo On Brominated Organic Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Artabe

Cunha-Silva

Barranco

2020

Food and Chemical Toxicology

View full text Add to dashboard Cite

Halogenated organic compounds are a particular group of contaminants consisting of a large number of substances, and of great concern due to their persistence in the environment, potential for bioaccumulation and toxicity. Some of these compounds have been classified as persistent organic pollutants (POPs) under The Stockholm Convention and many toxicity assessments have been conducted on them previously. In this work we provide an overview of enzymatic assays used in these studies to establish toxic effects and dose-response relationships. Studies in vivo and in vitro have been considered with a particular emphasis on the impact of halogenated compounds on the activity of relevant enzymes to the humans and the environment. Most information available in the literature focuses on chlorinated compounds, but brominated and fluorinated molecules are also the target of increasing numbers of studies. The enzymes identified can be classified as enzymes: i) the activities of which are affected by the presence of halogenated organic compounds, and ii) those involved in their metabolisation/detoxification resulting in increased activities. In both cases the halogen substituent seems to have an important role in the effects observed. Finally, the use of these enzymes in biosensing tools for monitoring of halogenated compounds is described.

show abstract

Section: Studies In Vivo On Brominated Organic Compoundsmentioning

confidence: 90%

Section: Studies In Vivo On Brominated Organic Compoundsmentioning

confidence: 99%

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Artabe

Cunha-Silva

Barranco

2020

Food and Chemical Toxicology

View full text Add to dashboard Cite

show abstract

“…Freezing stress might significantly increase the damage of the cell membrane. MDA content, together with EL, is a basic parameter to represent and examine the lipid peroxidation degree and the damage of cellular membranes under stress [35,36]. Our result showed that MDA content increased after the CA process (Figure 2).…”

Section: Discussionmentioning

confidence: 77%

Cold Acclimation Affects Physiological and Biochemical Characteristics of Betula platyphylla S. under Freezing Stress

Ritonga

Yan

Chen

et al. 2021

Forests

View full text Add to dashboard Cite

Cold and freezing stress is one of the most harmful environmental stresses, especially in temperate and subtropical areas, that adversely affects plant growth, development, and yield production. Betula platyphylla Sukaczev, also known as white birch, is one of the most valuable, important, and widely distributed tree species in East Asia. This study explored the effects of cold acclimation (CA) in reducing the destructive effect of freezing stress in B. platyphylla seedlings. We measured the physiological and biochemical characteristics of B. platyphylla seedlings, such as chlorophyll content, electrolyte leakage (EL), malondialdehyde (MDA), antioxidant enzymes (such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), and proline content before and after freezing stress to observe the contribution of CA in reducing the detrimental effects of freezing stress. The results showed that CA increased physiological and biochemical characteristics of B. platyphylla seedlings before and after freezing stress, except for chlorophyll content. Antioxidant enzymes were significantly positively correlated with proline, MDA, and EL content, and negatively correlated with chlorophyll content. Moreover, histochemical detection (H2O2 and O2−) and cell death were revealed to be induced by cold stress in B. platyphylla seedlings. Furthermore, it was revealed that increased time and decreased temperature of the CA process significantly influenced the physiological and biochemical parameters. Overall, the CA process significantly reduced the detrimental effects of freezing stress compared to the control treatment in B. platyphylla seedlings. Taken together, these findings provide beneficial information toward understanding the mechanism of CA and freezing stress in B. platyphylla. Furthermore, the substantial activity of physiological and biochemical results could be used as selection criteria for screening time and temperature points of cold/freezing stress in further omics analyses. In addition, the combination of current study results, further omics analyses, and genetic engineering techniques directly contribute to sustainable forest management systems, tree plantations, and conservation of tree species, especially non-cold/non-freezing tolerant tree species.

show abstract

“…SOD is an antioxidant enzyme, and is involved in the antioxidant system. cAT can neutralize excessive ROS production and catalyzes H 2 O 2 into H 2 O and O 2 (35). Thus, the levels of LdH, MdA, SOd and cAT were determined, and the results revealed that oxalate causes severe oxidative dysfunction, while miR-30c-5p mimic can relieve such an injury induced by oxalate.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of miR‑30c‑5p reduces cellular cytotoxicity and inhibits the formation of kidney stones through ATG5

et al. 2019

View full text Add to dashboard Cite

MicroRNAs (miRNAs or miRs) are critical regulators in various diseases. In the current study, the role of miR-30c-5p in the formation of sodium oxalate-induced kidney stones was investigated. For this purpose, human renal tubular epithelial cells (HK-2 cells) were incubated with sodium oxalate at the concentrations of 100, 250, 500, 750 and 1,000 µM. cell viability and the miR-30c-5p expression level were respectively measured by ccK-8 assay and RT-qPcR. After separately transfecting miR-30c-5p mimic and inhibitor into the HK-2 cells, the cell apoptotic rate, the levels of mitochondrial membrane potential (MMP) and ROS were determined by flow cytometry. The levels of oxidative stress indicators [lactate dehydrogenase (LdH), malondialdehyde (MdA), superoxide dismutase (SOd) and catalase (cAT)] were determined using commercial kits. crystal-cell adhesion assay was performed to evaluate the crystal adhesion capacity in vitro. miR-30c-5p binding at autophagy related 5 (ATG5) was predicted by TargetScan7.2 and further verified by dual-luciferase reporter assay. Rescue experiments were performed to confirm the molecular mechanisms underlying sodium oxalate-induced kidney formation in HK-2 cells. The results revealed that sodium oxalate decreased the viability of HK-2 cells in a concentration-dependent manner, and that miR-30c-5p expression was significantly downregulated by exposure to 750 µM sodium oxalate. In addition, the increase in cell apoptosis and crystal number, and the upregulated levels of LdH, MdA and ROS were reversed by the overexpression of miR-30c-5p. Moreover, the overexpression of miR-30c-5p upregulated the levels of SOd, cAT and MMP induced by sodium oxalate. ATG5 was directly regulated by miR-30c-5p, and the inhibition of cell cytotoxicity and crystal-cell adhesion induced by miR-30c-5p mimic was blocked by ATG5. These data indicated that the overexpression of miR-30c-5p alleviated cell cytotoxicity and crystal-cell adhesion induced by sodium oxalate through ATG5. Thus, the current study provides a better understanding of the role of miR-30c-5p in sodium oxalate-induced kidney stones.

show abstract

Insight into the tolerance, biochemical and antioxidative response in three moss species on exposure to BDE-47 and BDE-209

Cited by 14 publications

References 63 publications

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Enzymatic assays for the assessment of toxic effects of halogenated organic contaminants in water and food. A review

Cold Acclimation Affects Physiological and Biochemical Characteristics of Betula platyphylla S. under Freezing Stress

Overexpression of miR‑30c‑5p reduces cellular cytotoxicity and inhibits the formation of kidney stones through ATG5

Contact Info

Product

Resources

About