Insight into the stability of protein in confined environment through analyzing the structure of water by temperature-dependent near-infrared spectroscopy

Wang, Shiying; Wang, Mian; Han, Li; Sun, Yan; Cai, Wensheng; Shao, Xueguang

doi:10.1016/j.saa.2021.120581

Cited by 9 publications

(4 citation statements)

References 53 publications

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“…Water in a cell has different structures from that in bulk water because of the crowding and confined environment, of which the effect on the structure of biomolecules is considered to be the main reason for explaining the stability and activity of biomolecules [37]. To understand the function of water on the thermal stability of the proteins in a confined environment, the water structures in reverse micelles (RMs) were studied by temperature-dependent NIR spectroscopy [49]. The NIR spectra of aqueous solutions and RMs containing bovine serum albumin (BSA), HSA, and OVA were measured at different temperatures.…”

Section: Structural Change Of Water In the Aggregation Of Proteins An...mentioning

confidence: 99%

Chemometrics: An Excavator in Temperature-Dependent Near-Infrared Spectroscopy

2022

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Temperature-dependent near-infrared (NIR) spectroscopy has been developed and taken as a powerful technique for analyzing the structure of water and the interactions in aqueous systems. Due to the overlapping of the peaks in NIR spectra, it is difficult to obtain the spectral features showing the structures and interactions. Chemometrics, therefore, is adopted to improve the spectral resolution and extract spectral information from the temperature-dependent NIR spectra for structural and quantitative analysis. In this review, works on chemometric studies for analyzing temperature-dependent NIR spectra were summarized. The temperature-induced spectral features of water structures can be extracted from the spectra with the help of chemometrics. Using the spectral variation of water with the temperature, the structural changes of small molecules, proteins, thermo-responsive polymers, and their interactions with water in aqueous solutions can be demonstrated. Furthermore, quantitative models between the spectra and the temperature or concentration can be established using the spectral variations of water and applied to determine the compositions in aqueous mixtures.

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Section: Structural Change Of Water In the Aggregation Of Proteins An...mentioning

confidence: 99%

Chemometrics: An Excavator in Temperature-Dependent Near-Infrared Spectroscopy

2022

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“…To improve the spectral resolution, 27 continuous wavelet transforms (CWTs) were used to improve the spectral resolution and amplify the difference between the spectra. In this study, the symlet filter with a vanishing moment 2 (Sym2) at a scale parameter of 20 was adopted, which was approximately equivalent to the second derivative.…”

Section: Methodsmentioning

confidence: 99%

Detection and analysis of hyaluronic acid raw materials from different sources by NIR and aquaphotomics

Ma,

Guo,

Lin

et al. 2024

Anal. Methods

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“…For studying the water structures in a confinement environment, water in hydrogels and reverse micelles (RMs) were analyzed by NIR spectroscopy [76,77]. CWT was also adopted to enhance the resolution.…”

Section: Water As a Spectroscopic Probe For Quantitative And Structur...mentioning

confidence: 99%

Wavelet Transform Makes Water an Outstanding Near-Infrared Spectroscopic Probe

Wang

Cai

et al. 2023

Chemosensors

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Wavelet transform (WT) has been proven to be a powerful chemometric method for processing analytical data. In this review, works on the application of WT in processing near-infrared (NIR) spectrum were summarized, emphasizing the structural analysis of water in aqueous systems. The spectral features of water can be obtained from the resolution-enhanced NIR spectrum with the help of WT. Taking advantage of WT in resolution enhancement and the sensitivity of NIR spectroscopy for water, the spectral features for different water structures can be obtained, which makes water to be a potential NIR spectroscopic probe to detect the structural information of water and analyte in aqueous systems. Using the spectral variation of water with temperature, the interaction of water and solutes, and the role of water in chemical and bio-processes, such as the aggregation of proteins and polymers, was demonstrated. The spectral changes of the NIR spectrum with temperature were found able to reflect the structural changes of biomolecules or polymers in the analyzing systems.

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Insight into the stability of protein in confined environment through analyzing the structure of water by temperature-dependent near-infrared spectroscopy

Cited by 9 publications

References 53 publications

Chemometrics: An Excavator in Temperature-Dependent Near-Infrared Spectroscopy

Chemometrics: An Excavator in Temperature-Dependent Near-Infrared Spectroscopy

Detection and analysis of hyaluronic acid raw materials from different sources by NIR and aquaphotomics

Wavelet Transform Makes Water an Outstanding Near-Infrared Spectroscopic Probe

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