“…The value of the bimolecular quenching constant ( k q ) for C-Fe 3 O 4 NPs@OVA was measured by the relation k q = K sv /τ 0 and is found as 8.94, 7.66, 6.40 and 5.06 × 10 12 dm 3 mol −1 s −1 at 298, 303, 308, and 313 K, respectively. The calculated k q values for the complexation between C-Fe 3 O 4 NPs and OVA at all four temperatures were higher than the maximum diffusion collision rate constant (2.0 × 10 10 L mol −1 s −1 ), 12 revealing a strong complexation between them, probably a static type quenching mechanism. 34 In general, for a dynamic quenching process, the value of K SV increases due to the increase in number of collisions between the protein and NPs.…”