Insertion–deletion polymorphisms—utilization on forensic analysis

Francez, Pablo Abdon da Costa; Rodrigues, Elzemar Martins Ribeiro; Velasco, Afrânio Maurício de; Santos, Sidney Emanuel Batista dos

doi:10.1007/s00414-011-0588-z

Cited by 23 publications

(14 citation statements)

References 10 publications

(16 reference statements)

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“…In several studies, insertion–deletion (INDEL) markers have been used in population genetics and forensics [ 10 – 12 ] as it is easy to use. In the present study, we used INDEL to describe a unique 40 bp insertion in the HVR- I (control) region that is found mostly in the sambar population from the South India.…”

Section: Discussionmentioning

confidence: 99%

Detection of 40 bp insertion-deletion (INDEL) in mitochondrial control region among sambar (Rusa unicolor) populations in India

et al. 2015

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BackgroundThe mitochondrial DNA (mtDNA) control region is extensively used in the phylogeography of species. We examined sequence variations in the mtDNA control region of sambar (Rusa unicolor) populations from the South, Central and North India.ResultsMost of the samples collected from the south India exhibited a 40 bp insertion in the mtDNA control region. This insertion was not observed in the North and Central Indian populations.ConclusionThis study provided a potential marker for molecular screening and identification of sambar populations in the form of a distinct 40 bp insertion. Some populations in South India did not exhibit this insertion. It indicates that there could be an ecological barrier that might be preventing the expansion of insertion-positive sambar population.

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Section: Discussionmentioning

confidence: 99%

Detection of 40 bp insertion-deletion (INDEL) in mitochondrial control region among sambar (Rusa unicolor) populations in India

et al. 2015

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“…As the conventional diallelic Indels have only two alleles per locus, the smaller allele names S or 0, the larger one names L or 1 [10,17]. In this study, there were more than three alleles found for each multi-Indel markers, so it is very necessary to build up an organized nomenclature for the multiallelic Indels.…”

Section: Indel Nomenclaturementioning

confidence: 96%

A novel method for the analysis of 20 multi‐Indel polymorphisms and its forensic application

et al. 2013

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Insertion/deletion polymorphisms (Indels) have been considered as potential markers for forensic DNA analysis. However, the discrimination power of Indels is relatively lower due to the poor polymorphisms of diallelic markers. Here, two to three Indel loci that were very tightly linked in physical position were combined into a specific multi-Indel marker to improve the discrimination, as well as a multiplex that consisted of a set of multi-Indel markers was developed for forensic purpose. Finally, a multiplex system with 20 multi-Indel markers including 43 Indel loci from dbSNP database was constructed and DNA sample can be analyzed by this multiplex in one PCR reaction and one CE run. A total of 150 unrelated individuals from Hunan province in South-central China were genotyped by the multiplex system. The result showed that a total of 63 specific amplicons were detected, three alleles were observed in multi-Indel markers including two Indel loci and four alleles were observed in the markers including three Indel loci. The cumulative probability of exclusion and the accumulated discrimination power were 0.9989 and 0.9999999999994, respectively. Our result demonstrated that the strategy could be efficient to develop higher polymorphic multi-Indel markers, and the new multiplex could provide Supporting Information for forensic application.

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“…They are widely distributed throughout the genome and derived from single mutation events which occur at a very low frequency. Moreover, some of them might be informative as ancestry markers since they may display significant allele frequency differences among geographically separated populations, whereas others might be a complement in human identification tests [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Potential forensic use of a 33 X-InDel panel in the Argentinean population

et al. 2016

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Polymorphic genetic markers located on the X chromosome might become a complement in particular forensic identification when the biological kinship are deficient. We analyzed forensic statistical parameters of 33 X-chromosome InDel polymorphisms in a sample of 320 individuals from Argentina. The X-chromosome InDel polymorphism (X-InDel) panel was amplified in a single multiplex PCR reaction. Hardy-Weinberg Equilibrium was determined in the female cohort, whereas the male cohort was used to calculate linkage disequilibrium (LD) tested by an extension of Fisher's exact test, D', and Chi-square values. Regarding LD, 15 markers were organized and grouped into six blocks containing two or three linked loci each, namely block I (MID356-MID357), block II (MID448804-MID3703-MID218), block III (MID3705-MID3706-MID304737), block IV (MID197147-MID3754), block V (MID3664-MID284601-MID103547), and block VI (MID3763-MID3728). The haplotype diversity was higher than 0.99 in all cases. Blocks III and VI showed the highest match probability in the studied population, whereas block II showed the lowest. The accumulated power of discrimination was 99.9999991 % in women and 99.9992925 % in men. The mean exclusion chance in trios and duos were 99.9891736 and 99.6099391 %, respectively. Since 15 markers are associated as haplotypic blocks, for a conservative treatment of the data, statistical evaluation should consider their haplotypic frequencies and the remaining 18 markers can be evaluated as independent loci.

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Insertion–deletion polymorphisms—utilization on forensic analysis

Cited by 23 publications

References 10 publications

Detection of 40 bp insertion-deletion (INDEL) in mitochondrial control region among sambar (Rusa unicolor) populations in India

Detection of 40 bp insertion-deletion (INDEL) in mitochondrial control region among sambar (Rusa unicolor) populations in India

A novel method for the analysis of 20 multi‐Indel polymorphisms and its forensic application

Potential forensic use of a 33 X-InDel panel in the Argentinean population

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