Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti

Vontas, John; Kioulos, E.; Pavlidi, Nena; Morou, Evangelia; Torre, Alessandra della; Ranson, Hilary

doi:10.1016/j.pestbp.2012.05.008

Cited by 336 publications

(303 citation statements)

References 33 publications

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“…Because overexpression is frequently associated with overtranscription, most candidate genes were identified based on their differential transcription in resistant populations compared to susceptible counterparts using transcriptomics (for review, see David et al 2013). Although subjected to inherent difficulties associated with gene expression studies (e.g., RNA handling and degradation, uncontrolled variation across time, tissues, and populations), these approaches identified several detoxification enzymes overtranscribed in resistant populations, some of them being later validated as pyrethroid metabolizers (for review, see Vontas et al 2012;David et al 2013). Although these approaches proved their value for identifying metabolic resistance genes, they failed to pinpoint the genomic changes associated to their overexpression.…”

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confidence: 99%

Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

et al. 2015

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The capacity of mosquitoes to resist insecticides threatens the control of diseases such as dengue and malaria. Until alternative control tools are implemented, characterizing resistance mechanisms is crucial for managing resistance in natural populations. Insecticide biodegradation by detoxification enzymes is a common resistance mechanism; however, the genomic changes underlying this mechanism have rarely been identified, precluding individual resistance genotyping. In particular, the role of copy number variations (CNVs) and polymorphisms of detoxification enzymes have never been investigated at the genome level, although they can represent robust markers of metabolic resistance. In this context, we combined target enrichment with high-throughput sequencing for conducting the first comprehensive screening of gene amplifications and polymorphisms associated with insecticide resistance in mosquitoes. More than 760 candidate genes were captured and deep sequenced in several populations of the dengue mosquito Ae. aegypti displaying distinct genetic backgrounds and contrasted resistance levels to the insecticide deltamethrin. CNV analysis identified 41 gene amplifications associated with resistance, most affecting cytochrome P450s overtranscribed in resistant populations. Polymorphism analysis detected more than 30,000 variants and strong selection footprints in specific genomic regions. Combining Bayesian and allele frequency filtering approaches identified 55 nonsynonymous variants strongly associated with resistance. Both CNVs and polymorphisms were conserved within regions but differed across continents, confirming that genomic changes underlying metabolic resistance to insecticides are not universal. By identifying novel DNA markers of insecticide resistance, this study opens the way for tracking down metabolic changes developed by mosquitoes to resist insecticides within and among populations.

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Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

et al. 2015

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“…This is not a new phenomenon in mosquitoes, in which multiple insecticide resistance mechanisms has been reported worldwide [33,34].…”

Section: Resultsmentioning

confidence: 99%

Susceptibility of field-collected mosquitoes (Culex pipiens) in Northern Tunisia to temephos, an organophosphate insecticide

Daaboub¹,

Tabbabi²,

Lamari³

et al. 2017

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In order to understand the resistance of Culex pipiens to temephos and provide parameters for management programs, we evaluated the susceptibility levels to temephos of individuals collected in five localities of Northern Tunisia. Our results showed that sample # 2 was susceptible. The resistant samples displayed RR 50 ranged from 1.3 in sample # 5 to 440 in sample # 4. Mortality caused by propoxur ranged from 0% in sample # 4, which showed the highest resistance levels to studied temephos insecticide and indicated an important contribution of AChE 1, to 68% in sample # 5. Starch gel electrophoresis identified many esterases in studied samples with an important frequency (85%) in the sample # 4. This sample showed the highest resistance to temephos with a major contribution of CYP450, esterases, and AChE 1. Both detoxification mechanisms and target site alteration were involved in the resistance to temephos as reported in our study. This is not a new phenomenon in mosquitoes, in which multiple insecticide resistance mechanisms has been reported worldwide.

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“…S989P; I1,011M; I1,011V; V1,016I; V1,016G; F1,534C; and D1,794Y) have been described in Ae. aegypti (Vontas et al, 2012;Rinkevich, Du and Dong, 2013). Multiple kdr mutations had been reported from Mexico, the Caribbean, and Central America .…”

Section: Target-site Resistancementioning

confidence: 99%

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2018

Safety Assessment of Transgenic Organisms in the Environment, Volume 8

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This work is published under the responsibility of the Secretary-General of the OECD. The opinions expressed and arguments employed herein do not necessarily reflect the official views of OECD member countries.This document, as well as any data and any map included herein, are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area.Please cite this publication as: OECD (2018) The statistical data for Israel are supplied by and under the responsibility of the relevant Israeli authorities. The use of such data by the OECD is without prejudice to the status of the Golan Heights, East Jerusalem and Israeli settlements in the West Bank under the terms of international law.Photo credits: Cover © Maks Ershov.Corrigenda to OECD publications may be found on line at: www.oecd.org/about/publishing/corrigenda.htm. © OECD 2018You can copy, download or print OECD content for your own use, and you can include excerpts from OECD publications, databases and multimedia products in your own documents, presentations, blogs, websites and teaching materials, provided that suitable acknowledgment of the source and copyright owner(s) is given. All requests for public or commercial use and translation rights should be submitted to rights@oecd.org. Requests for permission to photocopy portions of this material for public or commercial use shall be addressed directly to the Copyright Clearance Center (CCC) at info@copyright.com or the Centre francais d'exploitation du droit de copie (CFC) at contact@cfcopies.com. FOREWORD │ 3SAFETY ASSESSMENTOF TRANSGENIC ORGANISMSIN THE ENVIRONMENT, VOLUME 8 © OECD 2018 ForewordModern biotechnologies are applied to plants species (crops, flowers, trees), animals and micro-organisms. The safety of the resulting transgenic organisms when released in the environment for their use in agriculture, forestry, the food and feed industry or for other applications represents a challenging issue. Genetically engineered products are rigorously assessed by their developers during their elaboration, and by governments when ready for release, to ensure high safety standards. This remains essential with new biotechnology developments using insects to fight against disease outbreaks: engineered mosquitoes need to be evaluated through a scientifically sound approach to risk/safety assessment that will inform biosafety regulators and support the decision concerning the release of these novel organisms in the environment.The OECD offers a long-standing recognised expertise in biosafety and contributes to facilitating an harmonised approach. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology (WG-HROB) was established in 1995. The WG-HROB gathers national authorities responsible for the environmental risk/safety assessment of products of modern biotechnology in OECD countries and other economies. International organisations and experts involved in biosafety activities are a...

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Insecticide resistance in the major dengue vectors Aedes albopictus and Aedes aegypti

Cited by 336 publications

References 33 publications

Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

Susceptibility of field-collected mosquitoes (Culex pipiens) in Northern Tunisia to temephos, an organophosphate insecticide

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