Insecticidal activity and mechanism of cinnamaldehyde in C. elegans

Lu, Lu; Shu, Chengjie; Chen, Lei; Yang, Yulong; Ma, Shihong; Zhu, Kai; Shi, Baojun

doi:10.1016/j.fitote.2020.104687

Cited by 16 publications

(18 citation statements)

References 29 publications

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“…According to our findings, cinnamaldehyde analogs have anthelmintic activity and 4-bromo and 4-chloro cinnamaldehydes were the most active at 10 and 20 μg/mL, respectively. Lu et al (2020) suggested that cinnamaldehyde disrupts glutathione metabolism and found that at 800 μg/mL caused 100% C. elegans mortality, which is several fold higher than the mortality rates observed for the cinnamaldehyde analogs tested in the present study (5–100 μg/mL). Slight surface morphological changes to the cuticle of C. elegans by 4-bromo and 4-chloro cinnamaldehydes confirmed their anthelmintic effects.…”

Section: Discussioncontrasting

confidence: 65%

“…Furthermore, trans -cinnamaldehyde has also been documented to have antibiofilm and anti-quorum sensing activity against Vibrio harveyi ( Niu et al, 2006 ), enterohemorrhagic and uropathogenic Escherichia coli strains ( Kim et al, 2015 ; Kot et al, 2015 ), methicillin-resistant Staphylococcus aureus strains ( Kavanaugh and Ribbeck, 2012 ; Kot et al, 2018 ), Pseudomonas aeruginosa ( Kavanaugh and Ribbeck, 2012 ), Pseudomonas fluorescens ( Li et al, 2018 ), Cronobacter sakazakii ( Amalaradjou and Venkitanarayanan, 2011 ), Streptococcus pyogenes ( Beema Shafreen et al, 2014 ), Salmonella typhimurium ( Silva et al, 2018 ), and against the pathogenic fungus C. albicans ( Ying et al, 2019 ; Miranda-Cadena et al, 2021 ). In addition, cinnamaldehyde analogs are known to have an array of bioactivities, which include antibacterial ( Firmino et al, 2018 ), antifungal ( Da Nobrega Alves et al, 2020 ), antiviral ( Hayashi et al, 2007 ), antiulcer ( Tabak et al, 1999 ), antioxidant ( Mathew and Abraham, 2006 ), antidiabetic ( Im et al, 2014 ), anti-inflammatory ( Srisook et al, 2019 ), anticancer ( Fang et al, 2004 ) activities and insecticidal ( Cheng et al, 2009 ; Lu et al, 2020 ), larvicidal ( Cheng et al, 2004 ), nematicidal ( Ferreira Barros et al, 2021 ), and anthelmintic ( Williams et al, 2015 ) effects.…”

Section: Introductionmentioning

confidence: 99%

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Appraisal of Cinnamaldehyde Analogs as Dual-Acting Antibiofilm and Anthelmintic Agents

et al. 2022

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Cinnamaldehyde has a broad range of biological activities, which include antibiofilm and anthelmintic activities. The ever-growing problem of drug resistance and limited treatment options have created an urgent demand for natural molecules with antibiofilm and anthelmintic properties. Hence, we hypothesized that molecules with a scaffold structurally similar to that of cinnamaldehyde might act as dual inhibitors against fungal biofilms and helminths. In this regard, eleven cinnamaldehyde analogs were tested to determine their effects on fungal Candida albicans biofilm and nematode Caenorhabditis elegans. α-Methyl and trans-4-methyl cinnamaldehydes efficiently inhibited C. albicans biofilm formation (>90% inhibition at 50 μg/mL) with minimum inhibitory concentrations (MICs) of ≥ 200 μg/mL and 4-bromo and 4-chloro cinnamaldehydes exhibited anthelmintic property at 20 μg/mL against C. elegans. α-Methyl and trans-4-methyl cinnamaldehydes inhibited hyphal growth and cell aggregation. Scanning electron microscopy was employed to determine the surface architecture of C. albicans biofilm and cuticle of C. elegans, and confocal laser scanning microscopy was used to determine biofilm characteristics. The perturbation in gene expression of C. albicans was investigated using qRT-PCR analysis and α-methyl and trans-4-methyl cinnamaldehydes exhibited down-regulation of ECE1, IFD6, RBT5, UCF1, and UME6 and up-regulation of CHT4 and YWP1. Additionally, molecular interaction of these two molecules with UCF1 and YWP1 were revealed by molecular docking simulation. Our observations collectively suggest α-methyl and trans-4-methyl cinnamaldehydes are potent biofilm inhibitors and that 4-bromo and 4-chloro cinnamaldehydes are anthelmintic agents. Efforts are required to determine the range of potential therapeutic applications of cinnamaldehyde analogs.

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Section: Discussioncontrasting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Appraisal of Cinnamaldehyde Analogs as Dual-Acting Antibiofilm and Anthelmintic Agents

et al. 2022

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“…Minc3s03593g34266 and Minc3s00365g11065, are orthologs to the same 3 C. elegans GSTs ( gst-6 , gst-33 , and gst-13 ). When exposed to cinnamaldehyde for just 4-h, gst -6 and 11 other GSTs were up-regulated in C. elegans 27 . A 72-h exposure to tris(1,3-dichloro-2-propyl), phosphate 10 GSTs were up-regulated including gst-6 and gst-33 in C. elegans 28 .…”

Section: Resultsmentioning

confidence: 96%

Transcriptional response of Meloidogyne incognita to non-fumigant nematicides

Wram

Hesse

Zasada

2022

Sci Rep

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There is limited research about the impacts of new nematicides, including fluazaindolizine, fluopyram, and fluensulfone, on the plant-parasitic nematode Meloidogyne incognita, despite it being a pervasive agricultural pest. In this study, M. incognita second-stage juveniles were exposed for 24-h to fluensulfone, fluazaindolizine, fluopyram, and oxamyl and total RNA was extracted and sequenced using next-generation sequencing to determine gene expression. The effects of nematicide exposure on cellular detoxification pathways, common differentially expressed (DE) genes, and fatty acid and retinol-binding genes were examined. Fluopyram and oxamyl had the smallest impacts on the M. incognita transcriptome with 48 and 151 genes that were DE, respectively. These compounds also elicited a weak response in the cellular detoxification pathway and fatty acid and retinol-binding (FAR) genes. Fluensulfone and fluazaindolizine produced robust transcriptional responses with 1208 and 2611 DE genes, respectively. These compounds had strong impacts on cellular detoxification, causing differential regulation of transcription factors and genes in the detox pathway. These compounds strongly down-regulated FAR genes between 52–85%. Having a greater understanding of how these compounds function at a molecular level will help to promote proper stewardship, aid with nematicide discovery, and help to stay a step ahead of nematicide resistance.

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“…Because CID is often used as the main component of insecticides, 26 special attention should be paid to the use of CID; otherwise, it will cause harm to crops, animals, and the soil ecological environment. 25 …”

Section: Resultsmentioning

confidence: 99%

“… 24 Because it is used as an effective component of common insecticides, its excessive use will cause some harm to the soil ecological environment. 25 , 26 It was found that CID had a strong inhibitory effect on Staphylococcus aureus and Escherichia coli ( E. coli ) 27 . In addition, studies have shown that Tribolium castaneum adults and Sitophilus zeamais adults were sensitive to contact toxicity of CID.…”

Section: Introductionmentioning

confidence: 99%

Study on the Combined Toxicities and Quantitative Characterization of Toxicity Sensitivities of Three Flavor Chemicals and Their Mixtures to Caenorhabditis elegans

et al. 2021

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It was shown that flavor chemicals with high toxicity sensitivities mean that small changes in their effective concentrations can lead to significant changes in toxicity. Flavors are widely used in personal care products. However, our study demonstrated that some flavor chemicals and their mixture rays have high toxicity sensitivities to Caenorhabditis elegans ( C. elegans ), which may have an impact on human health. In this paper, three flavor chemicals (benzyl alcohol, phenethyl alcohol, and cinnamaldehyde) were used as components of the mixture, and three binary mixture systems were constructed, respectively. Five mixture rays were designed for each mixture system by a direct equipartition ray design method. The lethal toxicities of the three flavor chemicals and mixture rays to C. elegans at three exposure volumes were determined. A new concept (inverse of the negative logarithmic concentration span (iSPAN)) was introduced to quantitatively evaluate the toxicity sensitivity of chemicals or mixture rays, and the combination index (CI) was employed to identify the toxicological interactions in the mixtures. It was shown that the three flavor chemicals as well as the binary mixture rays have a significant concentration–response relationship on the lethality of C. elegans . The iSPAN values of the three flavor chemicals and their mixture rays were larger than 3.000, showing very strong toxicity sensitivity to C. elegans . In mixture systems, the toxicity sensitivities of mixture rays with different mixture ratios were also different at different exposure volumes. In addition, it can be seen from the CI heat map that the toxicological interaction not only shows the mixture ratio dependence but also changes with the different exposure volumes, which implies that the mixtures consisting of flavor chemicals with high toxicity sensitivity have complex toxicological interactions. Therefore, in environmental risk assessment, special attention should be paid to chemicals with high toxicity sensitivities.

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Insecticidal activity and mechanism of cinnamaldehyde in C. elegans

Cited by 16 publications

References 29 publications

Appraisal of Cinnamaldehyde Analogs as Dual-Acting Antibiofilm and Anthelmintic Agents

Appraisal of Cinnamaldehyde Analogs as Dual-Acting Antibiofilm and Anthelmintic Agents

Transcriptional response of Meloidogyne incognita to non-fumigant nematicides

Study on the Combined Toxicities and Quantitative Characterization of Toxicity Sensitivities of Three Flavor Chemicals and Their Mixtures to Caenorhabditis elegans

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