Insect neuropeptide antagonists

Altstein, Miriam

doi:10.1002/1097-0282(2001)60:6<460::aid-bip10181>3.0.co;2-y

Cited by 32 publications

(11 citation statements)

References 82 publications

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“…The similarity between the inhibitory potency obtained by injection of the peptides in close proximity to the pheromone gland and that obtained by topical application indicates that penetration of these peptides through the cuticle is probably very high. Also interesting is the high persistence of the activity, which is consistent with the very high metabolic stability of the BBC peptides [22].…”

Section: Discussionmentioning

confidence: 83%

Bioavailability of backbone cyclic PK/PBAN neuropeptide antagonists – inhibition of sex pheromone biosynthesis elicited by the natural mechanism in Heliothis peltigera females

et al. 2010

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The bioavailability (i.e. ability to penetrate the insect cuticle, to reach the target organ and to exert bioactivity) of two backbone cyclic (BBC) pyrokinin/pheromone biosynthesis‐activating neuropeptide (PK/PBAN) antagonistic peptides was tested by applying them topically to Heliothis peltigera females and monitoring the resulting inhibition of sex pheromone production elicited by the natural (endogenous) mechanism during scotophase. Peptides were applied at various time points before the onset of scotophase, in aqueous or organic solvents, and pheromone content was examined at the 5th or 6th hour of scotophase. Both peptides penetrated the cuticle very efficiently and inhibited sex pheromone biosynthesis elicited by the natural mechanism for up to 8 or 9 h after application. The degree of inhibition differed between solvents: those applied in double‐distilled water (DDW) were more active than those applied in dimethylsulfoxide (inhibition by 53–73% and 15–38%, respectively, for BBC‐25, and 46–67% and 36–40%, respectively for BBC‐28). Peptides applied in dimethylsulfoxide and hexane exhibited slightly more persistent inhibitory activity than those applied in DDW. The solvents themselves did not affect sex pheromone production. Multiple applications (at −2, 0, +2 and +4 h) resulted in almost complete (87%) inhibition of sex pheromone biosynthesis, compared with 52% inhibition following a single application. The present study is the first demonstration of the ability of topically applied PK/PBAN antagonists to inhibit sex pheromone biosynthesis elicited by the natural mechanism in female moths, and provides important information on the bioavailability of BBC peptides and the mechanism responsible for sex pheromone production in these insects.

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Section: Discussionmentioning

confidence: 83%

Bioavailability of backbone cyclic PK/PBAN neuropeptide antagonists – inhibition of sex pheromone biosynthesis elicited by the natural mechanism in Heliothis peltigera females

et al. 2010

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“…Alstein et al have targeted the mating behavior of moths by developing PBAN antagonists that inhibit sex pheromone biosynthesis (for reviews, see [122,123]). Alstein et al have targeted the mating behavior of moths by developing PBAN antagonists that inhibit sex pheromone biosynthesis (for reviews, see [122,123]).…”

Section: Insect Peptide Hormones and Insect Controlmentioning

confidence: 99%

Insect Peptide Hormones

Isaac

Audsley

2009

Amino Acids, Peptides and Proteins in Organic Chemistry

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“…2,3 Hence, the use of neuropeptides to disrupt physiological processes in insects has long been considered as a viable basis for the development of alternative strategies for pest control. 4,5 Recent studies have documented the insecticidal effects of several insect neuropeptide analogues, such as kinin, 6,7 tachykinin, 8 diapause hormone, 9,10 and sulfakinin. 11 The Capability (CAP2b/CAPA-PK) peptides are multifunctional neuropeptides which include biologically active peptides of two types: periviscerokinin-like peptides (CAPA-PVK) and pyrokininlike peptides (CAPA-PK).…”

Section: Introductionmentioning

confidence: 99%

Assessment of insecticidal effects and selectivity of CAPA‐PK peptide analogues against the peach‐potato aphid and four beneficial insects following topical exposure

Gui

Taning

Schutter

et al. 2020

Pest Management Science

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BACKGROUND Insect Capability neuropeptides (CAP2b/CAPA‐PKs) play a critical role in modulating different physiologies and behavior in insects. In a previous proof‐of‐concept study, the CAP2b analogues 1895 (2Abf‐Suc‐FGPRLamide) and 2129 (2Abf‐Suc‐ATPRIamide) were reported to reduce aphid fitness when administered by injection. In the current study, the insecticidal efficacy of 1895 and 2129 on the peach potato aphid Myzus persicae was analyzed by topical application, simulating a spray application scenario in the field. Additionally, the selectivity of the tested analogues was evaluated against a selection of beneficial insects, namely three natural enemies (Adalia bipunctata, Chrysoperla carnea and Nasonia vitripennis) and a pollinator (Bombus terrestris). RESULTS Within 3–5 days post topical exposure of aphids to 1895, higher mortality (33%) was observed, as was the case for the treatment with 2129 (17%) and the mixture of 1895 + 2129 (47%) compared to the control (3%). 1895 and the mix 1895 + 2129 showed the strongest and comparable insecticidal effects. Additionally, surviving aphids treated with 1895 showed a reduction in total lifetime reproduction (GRR) of 30%, 19% with 2129 and 39% with the mix 1895 + 2129. Of interest from a biosafety perspective is that by using the same delivery method and dose, no significant effects on survival, weight increase and food intake was observed for the representative natural enemies and the pollinator. CONCLUSION This study highlights the potential of exploiting CAP2b analogues such as 1895 (core structure FGPRL) as aphicides. Additionally, the CAP2b analogues used in this study were selective as they showed no effects when applied on four representative beneficial insects.

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Insect neuropeptide antagonists

Cited by 32 publications

References 82 publications

Bioavailability of backbone cyclic PK/PBAN neuropeptide antagonists – inhibition of sex pheromone biosynthesis elicited by the natural mechanism in Heliothis peltigera females

Bioavailability of backbone cyclic PK/PBAN neuropeptide antagonists – inhibition of sex pheromone biosynthesis elicited by the natural mechanism in Heliothis peltigera females

Insect Peptide Hormones

Assessment of insecticidal effects and selectivity of CAPA‐PK peptide analogues against the peach‐potato aphid and four beneficial insects following topical exposure

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