Inosine supplementation effectively provokes the growth, immune response, oxidative stress resistance and intestinal morphology of juvenile red sea bream,Pagrus major

Abstract

A feeding trial was conducted to determine effects of dietary inosine on growth, immune responses, oxidative stress resistance and intestinal morphology of juvenile red sea bream. A semi‐purified basal diet supplemented with 0 (D1, control), 2 g kg−1 (D2), 4 g kg−1 (D3), 6 g kg−1 (D4) and 8 g kg−1 (D5) dietary inosine, respectively, to formulate five experimental diets. Each diet was randomly allocated to triplicate groups of fish (initial weight: 8 g). After 50 days of feeding trial, fish fed diets with 4 g kg−1 inosine had the highest (p < .05) final weight, weight gain and specific growth rate. Similarly, improved feed intake, feed conversion ratio and protein efficiency ratio were also found at 4 g kg−1 inosine supplemented group. Some non‐specific immune parameters such as total serum protein, lysozyme activity and bactericidal activity tended to be higher for fish fed diets supplemented with inosine. Peroxidase and catalase activity also influenced (p < .05) by dietary inosine supplementation. Fish fed 2 g kg−1 and 4 g kg−1 inosine supplemented diets showed the least oxidative stress condition. Inosine supplementation significantly increased (p < .05) anterior enterocyte height and posterior fold height, enterocyte height and microvillus height compared with control. We concluded that 4 g kg−1 dietary inosine supplementation effectively provokes growth and health performance of red sea bream by increasing growth, immune response, oxidative stress resistance and intestinal health condition.

Nucleoside by-product dietary supplementation influences blood chemistry, immune response, oxidative stress resistance and intestinal morphology of juvenile amberjack,Seriola dumerili

“…As NBP consisted considerable portion of inosine, the inosine as a nucleoside absorbed directly by intestine mostly and beneficially influenced the intestinal health of fish. The beneficial effects on intestine in the present study might be due to the positive effects of inosine on mucosa‐associated lymphoid tissue (MALT) which is a very important immune organ although the knowledge on MALT of fish is very limited (Hossain, Koshio, Ishikawa, Yokoyama, Sony, Usami, et al., ; Li & Gatlin, ).…”

Multiomics Provide Insights into the Key Molecules and Pathways Involved in the Physiological Adaptation of Atlantic Salmon (Salmo salar) to Chemotherapeutic-Induced Oxidative Stress

“…These molecules have known distinct roles in mitigating oxidative damage caused by ROS by protecting cells from DNA damage [ 36 ]. In particular, in fish, dietary inosine may confer oxidative stress resistance [ 37 ]. Therefore, the regulation of these two metabolites likely played a role in ameliorating the effects of the imbalanced internal ROS that resulted in oxidative stress, likely in a similar mechanism described in higher animal models.…”

Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics

“…Therefore, it must rely on salvage pathways as well [98]. If this is the case of E. leei , the possible therapeutic use of inosine against enteromyxosis is worth further investigation, since dietary inosine supplementation reduced the oxidative stress and improved intestinal health condition and immune response in several fish species [99, 100]. In fact, treatment with inosine compounds is currently being used for some human viral infections [101].…”