Inoculação artificial de Fusarium oxysporum em sementes de Phaseolus vulgaris

Rodrigues, Gustavo Fonseca; Migliorini, Patrícia; Junges, Emanuele; Silva, Raimunda Nonata Oliveira da; Chagas, H. L.; Nunes, Anita de Farias; Gularte, Jonas Albandes; Tunes, Lílian Vanussa Madruga de

doi:10.14808/sci.plena.2016.070202

Cited by 2 publications

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“…In turn, Rodrigues et al (2016) inoculated bean seeds with Fusarium oxysporum by exposing the seeds to the pathogen colony for 48 h under water restriction and observed a difference from the uninoculated control when evaluating the emergence at 10 d. However, there was no difference from the other inoculation methods, including substrate infestation with PDA disks containing pathogen mycelium and seed infestation by spore suspension.…”

Section: Resultsmentioning

confidence: 96%

“…The simplest seed inoculation methods are immersion in a spore suspension (Tanaka & Menten, 1991) and placing the seeds in contact with a fungal colony grown in conventional culture media. Seed inoculation with fungi or bacteria has been performed for different crops (Costa, Machado, Guimarães, Pozza, & Oride, 2003;Machado, Oliveira, Vieira, & Alves, 2004;Machado, Machado, Vieira, Cassetari Neto, & Souza, 2007;Rosa, 2006;Sousa et al, 2008;Farias, Del Ponte, Corrêa, Afonso, & Pierobom, 2010;Rodrigues et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Soybean Genotypes Resistant to Charcoal Rot by Seed Inoculation With Macrophomina phaseolina

Ishikawa¹,

Ribeiro²,

Almeida³

et al. 2019

JAS

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Soybean charcoal rot is a widespread root disease caused by the fungus Macrophomina phaseolina, a natural soil inhabitant that has great pathogenic variability and high survival capacity under adverse conditions. There are no registered fungicides or genotypes genetically resistant to this disease, although differences in susceptibility have been observed. As the fungus is a seed-borne pathogen, screening methods based on seed inoculation are quick and efficient. The objective of this work was to assess the efficiency of soybean seed infestation by incubation for 48 h with colonies of M. phaseolina, comparing two germination environment methods (germination paper or in pots with substrate), and to correlate the relative germination of genotypes of both methods with the severity of charcoal rot observed in a field test. The results showed that 48 h were sufficient to infest seeds and reduce the germination. The germination in paper was higher at 5 days after sowing (DAS) than that in pots at 8 DAS in a greenhouse. Both environments exhibited a highly negative correlation between seed germination and field disease severity (r = -0.775 in germination paper and r = -0.779 in pots with substrate). It is recommended that the germination test be performed in germination paper due to practicality and economy of space, material, and labor besides the better control of the environment.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Identification of Soybean Genotypes Resistant to Charcoal Rot by Seed Inoculation With Macrophomina phaseolina

Ishikawa¹,

Ribeiro²,

Almeida³

et al. 2019

JAS

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“…Osmotic conditioning (water restriction), also known as physiological conditioning, is another technique used in seed technology to potentiate the germination process of some species and in seed inoculation tests with fungi and bacteria (Pedroso et al 2010;Menezes et al 2011). However, these techniques may not provide conditions for the pathogen to infect the seeds, which requires adjustments such as the time of contact between them and the pathogen (Rodrigues et al 2016). Therefore, methodologies that allow pathogen transmission and infection via seeds to artificially inoculated seedlings must be standardized.…”

Section: Introductionmentioning

confidence: 99%

Colletotrichum truncatum transmission via lima bean seeds

Gomes,

Silva,

Medeiros

et al. 2023

Biosci. J.

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Anthracnose is the primary disease in Phaseolus lunatus cultures, causing severe losses. Inoculation techniques are vital for assessing genotype resistance and control methods at the early stages of seedling development. This study aimed to compare inoculation methods and exposure times of a lima bean seed variety to Colletotrichum truncatum using a completely randomized 4x5 factorial design with five replications. Seed inoculation methods by direct contact with mycelium, mannitol water restrictor, and sucrose water restrictor and immersion in conidia suspension were compared and submitted to substrates containing the developed pathogen or not at exposure times of 0, 36, 60, 84, and 108 hours. Evaluations were made by analyzing the severity, incidence, and disease index for anthracnose and seedling physiological quality under greenhouse conditions. The inoculation method by direct contact with sucrose solute for 36 hours was the most suitable for C. truncatum inoculation in lima bean seeds, providing a higher transmission rate but slightly affecting their physiological parameters. C. truncatum damage to lima bean seed performance increased with longer exposure times, regardless of the inoculation method.

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Inoculação artificial de Fusarium oxysporum em sementes de Phaseolus vulgaris

Cited by 2 publications

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Identification of Soybean Genotypes Resistant to Charcoal Rot by Seed Inoculation With Macrophomina phaseolina

Identification of Soybean Genotypes Resistant to Charcoal Rot by Seed Inoculation With Macrophomina phaseolina

Colletotrichum truncatum transmission via lima bean seeds

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