Injectable Solid Peptide Hydrogel as a Cell Carrier: Effects of Shear Flow on Hydrogels and Cell Payload

Yan, Congqi; Mackay, Michael E.; Czymmek, Kirk; Nagarkar, Radhika P.; Schneider, Joel P.; Pochan, Darrin J.

doi:10.1021/la2041746

Cited by 128 publications

(160 citation statements)

References 66 publications

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“…9,11,12 However, injectable hydrogels are often limited by a relatively low elastic modulus, [13][14][15][16] limiting their utility in applications demanding at least a degree of mechanical strength (e.g. engineering of stiffer tissues such as cartilage, 17 implantation in high-shear environments, 18 or spinal applications 19 ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Mechanical Properties in Cellulose Nanocrystal–Poly(oligoethylene glycol methacrylate) Injectable Nanocomposite Hydrogels through Control of Physical and Chemical Cross-Linking

2016

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While injectable hydrogels have several advantages in the context of biomedical use, their generally weak mechanical properties often limit their applications. Herein we describe in situgelling nanocomposite hydrogels based on poly(oligoethylene glycol methacrylate) (POEGMA) and rigid rod-like cellulose nanocrystals (CNCs) that can overcome this challenge. By physically incorporating CNCs into hydrazone cross-linked POEGMA hydrogels, macroscopic properties including gelation rate, swelling kinetics, mechanical properties, and hydrogel stability can be readily tailored. Strong adsorption of aldehyde and hydrazide modified POEGMA precursor polymers onto the surface of CNCs promotes uniform dispersion of CNCs within the hydrogel, imparts physical cross-links throughout the network, and significantly improves mechanical strength overall, as demonstrated by quartz crystal microbalance gravimetry and rheometry.When POEGMA hydrogels containing mixtures of long and short ethylene oxide side chain precursor polymers were prepared, transmission electron microscopy reveals that phase segregation occurs with CNCs hypothesized to preferentially locate within the stronger adsorbing short side chain polymer domains. Incorporating as little as 5 wt % CNCs results in dramatic enhancements in mechanical properties (up to 35-fold increases in storage modulus) coupled with faster gelation rates, decreased swelling ratios, and increased stability versus hydrolysis. Furthermore, cell viability can be maintained within 3D culture using these hydrogels independent of the CNC content. These properties collectively make POEGMA-CNC

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Section: Introductionmentioning

confidence: 99%

Enhanced Mechanical Properties in Cellulose Nanocrystal–Poly(oligoethylene glycol methacrylate) Injectable Nanocomposite Hydrogels through Control of Physical and Chemical Cross-Linking

2016

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“…This property is critical in defining the properties of hydrogel-encapsulated payload constructs where one would like to know the exact properties of the construct, e.g. drug delivery profile [96] or encapsulated cell state, after in vivo injection [73,97].…”

Section: Quaternary Structuresmentioning

confidence: 99%

“…Cytocompatibility is a broad, general term that describes whether or not a cell is able to survive near, in, with, or on a peptide hydrogel. Many peptide hydrogels, regardless of structure and composition, have shown good cytocompatibility with many different cell lines such as mesenchymal stem cells (MSCs) [73,97,98], embryonic stem cells (ESCs) [84,99], rat adrenal pheochromocytoma cells [100], or macrophages [101]. Many of these cell types have not only stayed alive while encapsulated, but also continued to grow and proliferate, indicating a high degree of cytocompatibility in the peptide hydrogels.…”

Section: Hydrogel Propertiesmentioning

confidence: 99%

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Peptidic Hydrogels

Sun¹,

Pochan²

2014

In-Situ Gelling Polymers

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This chapter looks into the hierarchical structures of peptide sequences and hydrogels constructed with physical solution assembly in an attempt to discuss the fundamental properties of peptide hydrogels and the molecular foundations. Peptide hydrogels are great candidates in the ever-growing field of biological and medical applications due to their ease of synthesis and customizable molecular and material features. The natural cytocompatibility and degradability of peptides make peptide hydrogels great candidates for cell encapsulation and drug delivery. There is immense potential in using new peptide molecules to make new hydrogel materials with both designed properties as well as unanticipated, excellent properties.Keywords Peptides · Injectable · Drug delivery · Cell encapsulation · Tissue engineering Within the increasingly expansive field of polymer hydrogels is the subset of peptide hydrogels. Peptides in nature and biology are critical for structure and function and involved in every bodily process from cell reproduction and tissue generation to simple enzymatic reactions. In the design of synthetic peptides, the scientist and engineer has twenty one natural amino acids in addition to a large and growing number of man-made amino acids to choose from for molecule formation. This amino acid toolbox provides for an almost limitless array of characteristics and function in new molecules and hydrogel materials. Amino acid attributes can include more traditional characteristics, such as hydrophobicity, polarity, or charge, or more exotic functionality such as the ability to crosslink with specificity in a "click" ligation reaction [1][2][3][4][5][6][7][8][9]. Therefore, these inherent

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“…15 It is the lack of shape specificity in the facial hydrophobic interactions between peptides that at least partially contributes to these defects and new branch/crosslink points. 11,12,[16][17][18] Thus, the facial and lateral hydrophobic interactions have a profound influence on the self-assembly kinetics, fibrillar nanostructure and network rheological properties of the MAX1 peptides.…”

Section: Introductionmentioning

confidence: 99%

ARO STIR: Defining Peptide Nanostructures By Engineering Assembly Interfaces

Sathaye¹,

Bhagwat²,

Pochan³

et al. 2013

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The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. This short-term innovative research proposal was focused on the structural characterization of peptide-based, self-assembled nanostructures of specific dimensions that are controlled by the geometry and physicochemical properties of the assembly interface. Our objectives were to (i) explore solution conditions that allow the production of nanostructures of select structural properties, specifically via the use of gradient hydrophobic interfaces, and (ii) characterize the conformation, fibril formation, and network properties of these peptide-based ABSTRACTThis short-term innovative research proposal was focused on the structural characterization of peptide-based, self-assembled nanostructures of specific dimensions that are controlled by the geometry and physicochemical properties of the assembly interface. Our objectives were to (i) explore solution conditions that allow the production of nanostructures of select structural properties, specifically via the use of gradient hydrophobic interfaces, and (ii) characterize the conformation, fibril formation, and network properties of these peptide-based materials via a suite of circular dichroic spectroscopy (CD), oscillatory rheology, microscopy (AFM, SEM, TEM), and scattering (SANS) methods.We have successfully achieved the aims of this STIR project, and have clearly demonstrated that (i) beta-hairpin structures can be formed by peptides with gradient hydrophobic faces comprising non-natural amino acids, (ii) these peptides are competent for fibril formation, and (iii) fibril formation and branching (as indicated by resulting network mechanical properties) can be modulated by modifications of the gradient of the hydrophobic interface. Loose packing of the designed fibrils is indicated, on the basis of initial SANS analysis, suggesting opportunities to modify the fibril interface with a range of chemically and electronically diverse hydrophobic amino acids, which will provide new opportunities to make smart materials for in-situ sensing and device fabrication. Number of Papers published in non peer-reviewed journals:No presentations on just this work to date. (c) Presentations Number of Presentations:0.00 Non Peer-Reviewed Conference Proceeding publications (other than abstracts):Received Names of Under Graduate students supported PERCENT_SUPPORTED NAME Graduate Students FTE Equivalent:Total Number:The number of undergraduates funded by this agreement who graduated during this period with a degree in science, mathematics, engineering, or technology fields:The number of undergraduates funded by your agreement who graduated during this period and will continue to pursue a graduate or Ph.D. degree in science, mathematics, engineering, or technology fields:Number of graduating unde...

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Injectable Solid Peptide Hydrogel as a Cell Carrier: Effects of Shear Flow on Hydrogels and Cell Payload

Cited by 128 publications

References 66 publications

Enhanced Mechanical Properties in Cellulose Nanocrystal–Poly(oligoethylene glycol methacrylate) Injectable Nanocomposite Hydrogels through Control of Physical and Chemical Cross-Linking

Enhanced Mechanical Properties in Cellulose Nanocrystal–Poly(oligoethylene glycol methacrylate) Injectable Nanocomposite Hydrogels through Control of Physical and Chemical Cross-Linking

Peptidic Hydrogels

ARO STIR: Defining Peptide Nanostructures By Engineering Assembly Interfaces

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