Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage

Chen, Yafang; Sui, Junhui; Wang, Qing; Yin, Yijia; Liu, Jun; Wang, Qiguang; Han, Xianglong; Sun, Yong; Fan, Yujiang; Zhang, Xingdong

doi:10.1016/j.carbpol.2018.02.057

Cited by 49 publications

(13 citation statements)

References 51 publications

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“…For this purpose, the Alg-Ph (1.5% w/v) and Alg-Ph/gelatin hydrogel samples (1.5%/1.5% w/v) were prepared in form of cylinder with a diameter of 14.6 mm and a height of 10 mm. The samples were then placed in an incubator in PBS with pH 7.4 at 37 C. The gels were then removed from the solution after 1, 3, 6, 18, 24, 48, and 72 hr and were weighed after a gentle drying of surface water by a filter paper (Chen et al, 2018;Moura, Faneca, Lima, Gil, & Figueiredo, 2011;Sakai & Kawakami, 2008). Swelling ratio was then obtained from Equation 1-2where W 0 and W 1 were initial and swelled weights of hydrogels at different time intervals (Balakrishnan, Mohanty, Umashankar, & Jayakrishnan, 2005;Moura et al, 2011).…”

Section: Swelling Ratiomentioning

confidence: 99%

Gelatin improves peroxidase‐mediated alginate hydrogel characteristics as a potential injectable hydrogel for soft tissue engineering applications

et al. 2020

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To develop an efficient injectable alginate‐based hydrogel for soft tissue engineering applications, phenol moiety (Ph) was introduced into alginate (Alg‐Ph), and the influence of gelatin as cell adhesive molecule was evaluated on the peroxidase‐mediated alginate hydrogel properties and cultured chondrocytic cell behavior. Addition of gelatin (1.5% w/v) to Alg‐Ph (1.5% w/v) hydrogels (Alg‐Ph/gelatin) regulated characteristics of the enzymatically gellable alginate hydrogel with increasing gelation time to 5.1 min (76%). Swelling ratio and degradation rates of the Alg‐Ph/gelatin hydrogel also increased 60 and 100%, respectively, while the mechanical strength value was 35% less than the Alg‐Ph hydrogel. Scanning electron microscopy images showed that the addition of gelatin could also increase uniformity of pore sizes inside the Alg‐Ph/gelatin hydrogels. The chondrocyte cells maintained their original phenotype and revealed statistically more metabolic activities in the Alg‐Ph/gelatin hydrogel. Hydrogels subscutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. Viable chondrocyte cells inside globular aggregates were seen as red colored areas in the cell‐laden hydrogels. The study demonstrates that enzymatically gellable alginate/gelatin hydrogel has fair potential as a natural‐based injectable hydrogel for soft tissue engineering applications.

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Section: Swelling Ratiomentioning

confidence: 99%

Gelatin improves peroxidase‐mediated alginate hydrogel characteristics as a potential injectable hydrogel for soft tissue engineering applications

et al. 2020

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“…Then, the swelling ratio (%) was calculated according to the formula after recording the weight of dry hydrogel (W 0 ) and the swelled hydrogel (W). 28…”

Section: Determination Of Swelling Ratio Water Retentionmentioning

confidence: 99%

<p>Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application</p>

Dong¹,

Zhuang²,

Hao³

et al. 2020

IJN

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Introduction: Chronic trauma repair is an important issue affecting people's healthy lives. Thermo-sensitive hydrogel is injectable in situ and can be used to treat large-area wounds. In addition, antioxidants play important roles in promoting wound repair. Methods: The purpose of this research was to prepare a novel thermo-sensitive hydrogelpoly(N-isopropyl-acrylamide)/poly(γ-glutamic acid) (PP) loaded with superoxide dismutase (SOD) to improve the effect for trauma treatment. The micromorphology of the hydrogel was observed by scanning electron microscope and the physical properties were measured. The biocompatibility of hydrogel was evaluated by MTT experiment, and the effect of hydrogel on skin wound healing was evaluated by in vivo histological staining. Results: Gelling behavior and differential scanning calorimeter outcomes showed that the PP hydrogels possessed thermo-sensitivity at physiological temperature and the phase transformation temperature was 28.2°C. The high swelling rate and good water retention were conducive to wound healing. The activity of SOD in vitro was up to 85% at 10 h, which was advantageous to eliminate the superoxide anion. MTT assay revealed that this hydrogel possessed good biocompatibility. Dressings of PP loaded with SOD (SOD-PP) had a higher wound closure rate than other treatments in vivo in diabetic rat model. Discussion: The SOD-PP thermo-sensitive hydrogels can effectively promote wound healing and have good application prospects for wound repair.

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“…The biomaterials used to fabricate scaffolds can be roughly divided into two main categories: synthetic polymers, including polycaprolactone (PCL), poly(lactic acid) (PLA), poly(glycolic acid) (PGA), poly(lactic-co-glycolic acid) (PLGA), and poly(L-lactic acid) (PLLA), among others. Alternatively, naturally derived biomaterials are for instance decellularized extracellular matrix (dECM) from diverse tissues and natural molecules such as collagen, gelatin, silk fibroin, HA, alginate, and chitosan, et al These materials can be used to fabricate different geometric shapes by 3D printing, electrospinning, injection molding techniques and among others ( Chen et al, 2018 ; Lukanina et al, 2018 ; Xia et al, 2018 ; Lu et al, 2019 ). The scaffolds fabricated using these two kinds of biomaterials have advantages and disadvantages ( Londono and Badylak, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

Liu

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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Biomaterials play a core role in cartilage repair and regeneration. The success or failure of an implanted biomaterial is largely dependent on host response following implantation. Host response has been considered to be influenced by numerous factors, such as immune components of materials, cytokines and inflammatory agents induced by implants. Both synthetic and native materials involve immune components, which are also termed as immunogenicity. Generally, the innate and adaptive immune system will be activated and various cytokines and inflammatory agents will be consequently released after biomaterials implantation, and further triggers host response to biomaterials. This will guide the constructive remolding process of damaged tissue. Therefore, biomaterial immunogenicity should be given more attention. Further understanding the specific biological mechanisms of host response to biomaterials and the effects of the host-biomaterial interaction may be beneficial to promote cartilage repair and regeneration. In this review, we summarized the characteristics of the host response to implants and the immunomodulatory properties of varied biomaterial. We hope this review will provide scientists with inspiration in cartilage regeneration by controlling immune components of biomaterials and modulating the immune system.

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Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage

Cited by 49 publications

References 51 publications

Gelatin improves peroxidase‐mediated alginate hydrogel characteristics as a potential injectable hydrogel for soft tissue engineering applications

Gelatin improves peroxidase‐mediated alginate hydrogel characteristics as a potential injectable hydrogel for soft tissue engineering applications

<p>Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application</p>

Host Response to Biomaterials for Cartilage Tissue Engineering: Key to Remodeling

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