Injectable angiogenic and osteogenic carrageenan nanocomposite hydrogel for bone tissue engineering

Yegappan, Ramanathan; Selvaprithiviraj, Vignesh; Amirthalingam, Sivashanmugam; Mohandas, Annapoorna; Hwang, Nathaniel S.; Jayakumar, R.

doi:10.1016/j.ijbiomac.2018.10.182

Cited by 84 publications

(59 citation statements)

References 26 publications

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“…β-TCP was once considered as bone WH due to its close structural resemblance with WH. However, recent powder X-ray diffraction (PXRD) and SEM analysis of bone have revealed that it is WH that exists naturally in the bone while β-TCP is merely a synthetic analog with similar structure [9][10][11]. Due to biological importance, presence of hydrogen and magnesium makes WH different from β-TCP.…”

Section: Of 14mentioning

confidence: 99%

Synthesis, Characterization and Process Optimization of Bone Whitlockite

et al. 2020

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Whitlockite, being the second most abundant bio-mineral in living bone, finds huge applications in tissue regeneration and implants and its synthesis into its pure form has remained a challenge. Although precipitation of whitlockite phase has been reported recently in many publications, effects of various parameters to control such phase as well as conditions for the bulk preparation of this extremely important bio-mineral have not been investigated so far. In this work, we report the precipitation of pure whitlockite phase using common precursors. As reported in the literature, whitlockite is stable in a narrow pH range, therefore; optimization of pH for the stabilization of whitlockite phase has been investigated. Additionally, in order to narrow down the optimum conditions for the whitlockite precipitation, effect of temperature and heating conditions has also been studied. The obtained solids were characterized using powder X-ray diffraction (PXRD), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). From PXRD analysis, it was observed that heating the precursor’s mixture at 100 °C with subsequent aging at the optimized pH resulted in the precipitation of pure whitlockite phase. These results were further confirmed by TGA, SEM and Raman spectroscopy analysis and it was confirmed that the conditions reported here favor whitlockite precipitation without formation of any secondary phase. These reaction conditions were further confirmed by changing all the parameters like aging, heating time, feed rate of precursors one by one. From PXRD analysis of these samples, it was concluded that not only pH but temperature, heating time, aging time and feed rate effect simultaneously on the precipitation of pure whitlockite phase and a subtle change in any of these parameters could lead to the formation of undesired stable secondary calcium phosphate phases.

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Section: Of 14mentioning

confidence: 99%

Synthesis, Characterization and Process Optimization of Bone Whitlockite

et al. 2020

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“…In this study, XG improved in vitro proliferation of ADMSCs significantly in a dose-dependent manner and a Good injectability, mechanical stability, and good cytocompatibility of CCG hydrogels reinforced with whitlockite NPs and dimethyloxallylglycine (an angiogenic drug) with human umbilical vein endothelial cells were observed. Overall, CCG hydrogels promoted osteogenesis and angiogenesis in vitro [63]. In another study, the addition of 0.5% GuG improved the hydrogen bonding of soybean protein isolate (SPI) adhesives with a maximal bond strength of 5% SPI adhesive and that was 2.8-fold higher than those of control.…”

Section: Osteoarthritis and Cartilage Tissue Engineeringmentioning

confidence: 91%

“…− functional groups, high water-absorption capacity, and other desirable physico-chemical characteristics [63]. Guar Gum (GuG) is a water-soluble and non-ionic galactomannan high molecular weight plant polysaccharide that is extracted from Cyampsis tetragonolobus seeds [64] and is composed of linear chains of β-(1-4)-D-mannan, having side chains of α-(1-6) linked galactose [65].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review

et al. 2020

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The engineering of tissues under a three-dimensional (3D) microenvironment is a great challenge and needs a suitable supporting biomaterial-based scaffold that may facilitate cell attachment, spreading, proliferation, migration, and differentiation for proper tissue regeneration or organ reconstruction. Polysaccharides as natural polymers promise great potential in the preparation of a three-dimensional artificial extracellular matrix (ECM) (i.e., hydrogel) via various processing methods and conditions. Natural polymers, especially gums, based upon hydrogel systems, provide similarities largely with the native ECM and excellent biological response. Here, we review the origin and physico-chemical characteristics of potentially used natural gums. In addition, various forms of scaffolds (e.g., nanofibrous, 3D printed-constructs) based on gums and their efficacy in 3D cell culture and various tissue regenerations such as bone, osteoarthritis and cartilage, skin/wound, retinal, neural, and other tissues are discussed. Finally, the advantages and limitations of natural gums are precisely described for future perspectives in tissue engineering and regenerative medicine in the concluding remarks.

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“…Based on direct mixing and co-precipitation principles respectively, the suspension and immersion techniques are the main manufacturing methods to integrate bioceramics into collagen (Kikuchi et al, 2004;Yunoki et al, 2007;Xia et al, 2016). -Need for determining aquaculture systems or farming (when ex situ cultivation is difficult) -Species-dependent variability of characteristics and composition (Green et al, 2003;Granito et al, 2016) Heparin -Preserve the growth factor stability and bioactivity -Reduced cell growth rate (Chung and Park, 2007) Polysaccharide-based polymers (Khan and Ahmad, 2013;Sofi et al, 2018) Carrageenans-High molecular flexibility -Thixotropic nature -Gel dissolution in the absence of a gel-inducing reagent -Elevated melting temperature (Garg et al, 2012;Yegappan et al, 2019) Gellan gum -Resistance to acidic conditions and high temperature -Transparency, flexibility and elasticity in the highly acylic gels -Low elasticity and brittleness when used in the low acyl form (Pereira et al, 2013;Manda et al, 2018) Some examples of collagen biocomposites and related properties are reported in Table 4. It is well-established that adding HA not only increases the compression modulus of collagen scaffolds, but also provides a larger and rougher adherence surface allowing for improved adhesion, bioactivity, and proliferation of cells (Sionkowska and Kozlowska, 2013).…”

Section: Biocompositesmentioning

confidence: 99%

Natural Polymeric Scaffolds in Bone Regeneration

Filippi

Born

Chaaban

et al. 2020

Front. Bioeng. Biotechnol.

248

151

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Despite considerable advances in microsurgical techniques over the past decades, bone tissue remains a challenging arena to obtain a satisfying functional and structural restoration after damage. Through the production of substituting materials mimicking the physical and biological properties of the healthy tissue, tissue engineering strategies address an urgent clinical need for therapeutic alternatives to bone autografts. By virtue of their structural versatility, polymers have a predominant role in generating the biodegradable matrices that hold the cells in situ to sustain the growth of new tissue until integration into the transplantation area (i.e., scaffolds). As compared to synthetic ones, polymers of natural origin generally present superior biocompatibility and bioactivity. Their assembly and further engineering give rise to a wide plethora of advanced supporting materials, accounting for systems based on hydrogels or scaffolds with either fibrous or porous architecture. The present review offers an overview of the various types of natural polymers currently adopted in bone tissue engineering, describing their manufacturing techniques and procedures of functionalization with active biomolecules, and listing the advantages and disadvantages in their respective use in order to critically compare their actual applicability potential. Their combination to other classes of materials (such as micro and nanomaterials) and other innovative strategies to reproduce physiological bone microenvironments in a more faithful way are also illustrated. The regeneration outcomes achieved in vitro and in vivo when the scaffolds are enriched with different cell types, as well as the preliminary clinical applications are presented, before the prospects in this research field are finally discussed. The collection of studies herein considered confirms that advances in natural polymer research will be determinant in designing translatable materials for efficient tissue regeneration with forthcoming impact expected in the treatment of bone defects.

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Injectable angiogenic and osteogenic carrageenan nanocomposite hydrogel for bone tissue engineering

Cited by 84 publications

References 26 publications

Synthesis, Characterization and Process Optimization of Bone Whitlockite

Synthesis, Characterization and Process Optimization of Bone Whitlockite

Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review

Natural Polymeric Scaffolds in Bone Regeneration

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